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The map label for this gene is hsdM [C]

Identifier: 13508081

GI number: 13508081

Start: 407861

End: 409492

Strand: Direct

Name: hsdM [C]

Synonym: MPN342

Alternate gene names: 13508081

Gene position: 407861-409492 (Clockwise)

Preceding gene: 13508080

Following gene: 13508082

Centisome position: 49.96

GC content: 32.84

Gene sequence:

>1632_bases
ATGGAAAAGAAACGTACTGAACAACGTAATGGCGTTGAAAAGAAGATTTGAGAAATAGCTGATAAATTAAGGGGAACCAT
TGATGGATGGGACTTTAAAAGCTATGTTTTAATTGGTCTTTTTTACCGTTTTCTTTCCGAAAACCTATGCAAATATTTTA
ACGATTCTGAAAGAAGGAATAATCCTGATTTTAGTTACGAAAATTTAACAGATGATTATGAAGCAATTGATGCATTAAAA
GATGCTGCCATTGCAAGTAAAGGCTTTTTTATTAAGCCATCTCAGCTTTTTCAAAATGTTGTTAAAAGTATTCGTGAAAA
TAAAAATAACGAAGATCTAAATACAACTTTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAA
GAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGA
ACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATT
TGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAG
ATATATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGT
GGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAA
CCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTG
ATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAAT
CCACCATATTCCATTAGTTGAGCTGGTGATAAAAAATCCAATTTAGTAAGTGACGTTCGTTTTAAAGATGCAGGGACATT
GGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTG
TTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGAT
GCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGA
TAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAA
ATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAGAAGAAGCTAATTTTGCAAAGTCGGTTGAAAGGGACAAAATT
AGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTT
GAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAG
TAGATTTTGACAACATTGATATTAATAACTAG

Upstream 100 bases:

>100_bases
TGAACAAGTTTTTACCAATTTAATAACTAAAATTAAAATCCTTATTAATTGTTAGTAATAACAACTTTCAACATAACTAA
ATAACTAAACTCACTATATC

Downstream 100 bases:

>100_bases
TTGATAATAAAGCAATTCTTTGTTAATTTACAAGAATTTAAAAACTAATAAATTAATTTCATTATTGTTGTGCAAATTAA
AACTTACAAAATCAAGGATA

Product: type I restriction enzyme HsdM

Products: NA

Alternate protein names: M.MpnORFDP [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MEKKRTEQRNGVEKKIWEIADKLRGTIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNNPDFSYENLTDDYEAIDALK
DAAIASKGFFIKPSQLFQNVVKSIRENKNNEDLNTTLRDIFDDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIR
TEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGS
GSLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEWEKHYVNCSNENGFEVVVSN
PPYSISWAGDKKSNLVSDVRFKDAGTLAPNSKADLAFVLHALYVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVD
AVIQLPSNLFSTTSIATSILVLKKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKI
RESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDINN

Sequences:

>Translated_543_residues
MEKKRTEQRNGVEKKI*EIADKLRGTIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNNPDFSYENLTDDYEAIDALK
DAAIASKGFFIKPSQLFQNVVKSIRENKNNEDLNTTLRDIFDDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIR
TEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGS
GSLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEWEKHYVNCSNENGFEVVVSN
PPYSIS*AGDKKSNLVSDVRFKDAGTLAPNSKADLAFVLHALYVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVD
AVIQLPSNLFSTTSIATSILVLKKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKI
RESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDINN
>Mature_543_residues
MEKKRTEQRNGVEKKI*EIADKLRGTIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNNPDFSYENLTDDYEAIDALK
DAAIASKGFFIKPSQLFQNVVKSIRENKNNEDLNTTLRDIFDDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIR
TEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGS
GSLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEWEKHYVNCSNENGFEVVVSN
PPYSIS*AGDKKSNLVSDVRFKDAGTLAPNSKADLAFVLHALYVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVD
AVIQLPSNLFSTTSIATSILVLKKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKI
RESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDINN

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=286, Percent_Identity=24.1258741258741, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 61567; Mature: 61567

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKRTEQRNGVEKKIEIADKLRGTIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNN
CCCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
PDFSYENLTDDYEAIDALKDAAIASKGFFIKPSQLFQNVVKSIRENKNNEDLNTTLRDIF
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIRTEKLKELLTSIDTMELDEFEK
HHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHC
NSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGSG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCH
SLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEW
HHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHH
EKHYVNCSNENGFEVVVSNPPYSISAGDKKSNLVSDVRFKDAGTLAPNSKADLAFVLHAL
HHHHCCCCCCCCCEEEECCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
YVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSNLFSTTSIATSILVL
HHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEE
KKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKIRE
ECCCCCCCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
SNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDIN
CCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
N
C
>Mature Secondary Structure
MEKKRTEQRNGVEKKIEIADKLRGTIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNN
CCCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
PDFSYENLTDDYEAIDALKDAAIASKGFFIKPSQLFQNVVKSIRENKNNEDLNTTLRDIF
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIRTEKLKELLTSIDTMELDEFEK
HHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHC
NSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGSG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCH
SLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEW
HHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHH
EKHYVNCSNENGFEVVVSNPPYSISAGDKKSNLVSDVRFKDAGTLAPNSKADLAFVLHAL
HHHHCCCCCCCCCEEEECCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
YVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSNLFSTTSIATSILVL
HHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEE
KKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKIRE
ECCCCCCCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
SNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDIN
CCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]