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The map label for this gene is mutB1 [H]

Identifier: 13508080

GI number: 13508080

Start: 405573

End: 407720

Strand: Direct

Name: mutB1 [H]

Synonym: MPN341

Alternate gene names: 13508080

Gene position: 405573-407720 (Clockwise)

Preceding gene: 13508079

Following gene: 13508081

Centisome position: 49.68

GC content: 35.43

Gene sequence:

>2148_bases
ATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTA
TTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTA
GCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAG
TATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGT
GATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTA
TTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAG
TTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGG
TAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTAT
TGCGCAAGTGATCGAAAAAATTTCAGTACATTTTAGGTGATGAGTTTCAAGATACCAACCAAATTCAGTATGAACTAATT
AAGATGTTGGCCAGCCATCACCAAAACCTTTTTTTAGTGGGTGACAATAACCAGATGATTTACCGCTGAAGGGGCGCGGT
TAGTGACATTATTGATTCTTTAAAAAGTGATTTTAAGGTAAGACCCGAAAACGAGTTTTACATTACCCAAAACTACCGTT
GTGATCAGAACATCTTAACGGTGGCAAACTCGATTCTAGGCACCATTTATGCCAAAGAAAACCAACCTGATTCAACTAAA
GGCTTTCTTTTTTCTGCCATTAAATCAAACCGGCTTCCAGTTTATTTCCAAGCCTCTTCAGTTGAAGGCCAACATTCTTG
AATTATCAATAAGATTAAGAATTTACATAAAAACCATGGCATTCAATACAAAGACATGGCTATTTTGTTTCGCACCAACA
GAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAG
TTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCG
GGTTTGACCTAATGTTTTAAATAAAAGTCTCACTGTAAACGAGCAAGTGAATTTAGATAAAATCTTGCAAAACTTAGAGC
AAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTT
GAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTT
TAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAG
ATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTC
TTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGA
ACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAA
TTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCAC
CAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAA
TGGTGACTCTGTTAGCCACCGTACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTA
AAAACAGTAAGTACGGACAGAGGTGAATTGTTAAAAATCACCGTGATTTGGTGAAGGCTGTGTATTAG

Upstream 100 bases:

>100_bases
TTATTACCGCTGTGTTAAAACTTTTTGTTGAAAATTAACATTTTATCCCTTTTTTATAACAGATTACACTAATTAAAAAA
ACAATCTTAAACCTAGTACT

Downstream 100 bases:

>100_bases
TTATGATCTGCTATTAAAAAAGAAATTGTAATAATAAAAATGAACAAGTTTTTACCAATTTAATAACTAAAATTAAAATC
CTTATTAATTGTTAGTAATA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEK
YHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCE
FHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRKWSKKFQYILGDEFQDTNQIQYELI
KMLASHHQNLFLVGDNNQMIYRWRGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVANSILGTIYAKENQPDSTK
GFLFSAIKSNRLPVYFQASSVEGQHSWIINKIKNLHKNHGIQYKDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQ
LETFKKVLVALITRSNYDIKLALKSLRVWPNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFV
EEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAV
FIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVH
QDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQRWIVKNHRDLVKAVY

Sequences:

>Translated_715_residues
MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEK
YHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCE
FHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRK*SKKFQYILGDEFQDTNQIQYELI
KMLASHHQNLFLVGDNNQMIYR*RGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVANSILGTIYAKENQPDSTK
GFLFSAIKSNRLPVYFQASSVEGQHS*IINKIKNLHKNHGIQYKDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQ
LETFKKVLVALITRSNYDIKLALKSLRV*PNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFV
EEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAV
FIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVH
QDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQR*IVKNHRDLVKAVY
>Mature_714_residues
AFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKY
HKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEF
HLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRK*SKKFQYILGDEFQDTNQIQYELIK
MLASHHQNLFLVGDNNQMIYR*RGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVANSILGTIYAKENQPDSTKG
FLFSAIKSNRLPVYFQASSVEGQHS*IINKIKNLHKNHGIQYKDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQL
ETFKKVLVALITRSNYDIKLALKSLRV*PNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVE
EQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVF
IYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQ
DDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQR*IVKNHRDLVKAVY

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=724, Percent_Identity=27.7624309392265, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI48994965, Length=677, Percent_Identity=27.7695716395864, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1787196, Length=341, Percent_Identity=27.5659824046921, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=531, Percent_Identity=27.683615819209, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82477; Mature: 82346

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALT
CEECCCCCCCCHHHHHEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHEEEEE
YTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFL
ECHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
KESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAA
HHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRKSKKFQYILGDEFQDTNQIQYELIK
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
MLASHHQNLFLVGDNNQMIYRRGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVA
HHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHCCEECCCCCEEEEECCCCCHHHHHHH
NSILGTIYAKENQPDSTKGFLFSAIKSNRLPVYFQASSVEGQHSIINKIKNLHKNHGIQY
HHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCE
KDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLAL
EEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEH
KSLRVPNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQ
HHCCCCHHHCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHCCCCEE
NLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFL
EEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTD
HHHHHHCCCEECHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCHHHHHHHHCCCCC
FVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQRIVKNHRDLVKAVY
CCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALT
EECCCCCCCCHHHHHEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHEEEEE
YTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFL
ECHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
KESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAA
HHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRKSKKFQYILGDEFQDTNQIQYELIK
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
MLASHHQNLFLVGDNNQMIYRRGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVA
HHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHCCEECCCCCEEEEECCCCCHHHHHHH
NSILGTIYAKENQPDSTKGFLFSAIKSNRLPVYFQASSVEGQHSIINKIKNLHKNHGIQY
HHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCE
KDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLAL
EEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEH
KSLRVPNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQ
HHCCCCHHHCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHCCCCEE
NLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFL
EEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTD
HHHHHHCCCEECHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCHHHHHHHHCCCCC
FVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQRIVKNHRDLVKAVY
CCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]