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The map label for this gene is dhfr [H]

Identifier: 13508060

GI number: 13508060

Start: 381529

End: 382011

Strand: Direct

Name: dhfr [H]

Synonym: MPN321

Alternate gene names: 13508060

Gene position: 381529-382011 (Clockwise)

Preceding gene: 161723289

Following gene: 13508061

Centisome position: 46.73

GC content: 42.24

Gene sequence:

>483_bases
ATGGTAAAAGCAATTTGAGCAATGGATCAAAATGGGTTAATTGGTAACGGTAACAGTTTGCCCTGAAGGATTAAAGCGGA
GTTACAGCACTTTCGCCAGACCACGTTGCACCAAGATGTATTAATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGT
TTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATC
CATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTA
TGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAG
TGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGA
TAA

Upstream 100 bases:

>100_bases
TTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCTACAATTAA
AGGAGAGGTAGCAGTTTAAG

Downstream 100 bases:

>100_bases
AACAATATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAA
CTTGCGCTCGGTTAACTGAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MVKAIWAMDQNGLIGNGNSLPWRIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVI
HEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR

Sequences:

>Translated_160_residues
MVKAI*AMDQNGLIGNGNSLP*RIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVI
HEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR
>Mature_160_residues
MVKAI*AMDQNGLIGNGNSLP*RIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVI
HEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18103; Mature: 18103

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKAIAMDQNGLIGNGNSLPRIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYIL
CCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
TRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTI
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
FGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR
HHCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKAIAMDQNGLIGNGNSLPRIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYIL
CCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
TRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTI
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
FGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR
HHCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]