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The map label for this gene is nrdF [H]

Identifier: 13508061

GI number: 13508061

Start: 382018

End: 383037

Strand: Direct

Name: nrdF [H]

Synonym: MPN322

Alternate gene names: 13508061

Gene position: 382018-383037 (Clockwise)

Preceding gene: 13508060

Following gene: 13508062

Centisome position: 46.79

GC content: 40.88

Gene sequence:

>1020_bases
ATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCG
CTCGGTTAACTGAAACCTAGTGGATGATGAGAAGGATCTGGAAGTGTGAAACCGGATCACGCAAAACTTCTGGTTACCAG
AAAAGATTCCGGTATCCAATGACATTCCATCATGAAAGCAGCTGTCTAAGGAATGGCAGGATTTAATTACCAAAACCTTT
ACGGGGTTAACATTACTTGACACCATCCAAGCTACCATTGGTGACATTAAGCAAATTGACTATGCTTTAACAGATCACGA
GCAGGTAATTTACGCCAACTTTGCCTTTATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTA
CTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCC
TATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTA
TTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGACATTATTCGCTTAATTTTGCGCGATAAGGTAA
TCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGG
TTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGT
AGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAG
AAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAAT
GGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGAGACTTTTAA

Upstream 100 bases:

>100_bases
ATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACA
AAATTGCACGATAAAACAAT

Downstream 100 bases:

>100_bases
CAATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCC
TCCATTTCTAATAACACCCA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MANTKKYFLESVSPLEYAQKKFQGNLRSVNWNLVDDEKDLEVWNRITQNFWLPEKIPVSNDIPSWKQLSKEWQDLITKTF
TGLTLLDTIQATIGDIKQIDYALTDHEQVIYANFAFMVGVHARSYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIP
YYTGKDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKR
FVFDLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIFAQLSARADENHDFFSGN
GSSYVMGISEETEDKDWDF

Sequences:

>Translated_339_residues
MANTKKYFLESVSPLEYAQKKFQGNLRSVN*NLVDDEKDLEV*NRITQNFWLPEKIPVSNDIPS*KQLSKEWQDLITKTF
TGLTLLDTIQATIGDIKQIDYALTDHEQVIYANFAFMVGVHARSYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIP
YYTGKDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKR
FVFDLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIFAQLSARADENHDFFSGN
GSSYVMGISEETEDKD*DF
>Mature_338_residues
ANTKKYFLESVSPLEYAQKKFQGNLRSVN*NLVDDEKDLEV*NRITQNFWLPEKIPVSNDIPS*KQLSKEWQDLITKTFT
GLTLLDTIQATIGDIKQIDYALTDHEQVIYANFAFMVGVHARSYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIPY
YTGKDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKRF
VFDLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIFAQLSARADENHDFFSGNG
SSYVMGISEETEDKD*DF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=305, Percent_Identity=51.1475409836066, Blast_Score=317, Evalue=7e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38596; Mature: 38465

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTKKYFLESVSPLEYAQKKFQGNLRSVNNLVDDEKDLEVNRITQNFWLPEKIPVSNDI
CCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCC
PSKQLSKEWQDLITKTFTGLTLLDTIQATIGDIKQIDYALTDHEQVIYANFAFMVGVHAR
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
SYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIPYYTGKDPLKSKVAAALMPGFLLY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKRFVF
HHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
DLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHH
AQLSARADENHDFFSGNGSSYVMGISEETEDKDDF
HHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ANTKKYFLESVSPLEYAQKKFQGNLRSVNNLVDDEKDLEVNRITQNFWLPEKIPVSNDI
CCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCC
PSKQLSKEWQDLITKTFTGLTLLDTIQATIGDIKQIDYALTDHEQVIYANFAFMVGVHAR
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
SYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIPYYTGKDPLKSKVAAALMPGFLLY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKRFVF
HHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
DLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHH
AQLSARADENHDFFSGNGSSYVMGISEETEDKDDF
HHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]