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The map label for this gene is asnC [H]

Identifier: 13507991

GI number: 13507991

Start: 303037

End: 304404

Strand: Direct

Name: asnC [H]

Synonym: MPN252

Alternate gene names: 13507991

Gene position: 303037-304404 (Clockwise)

Preceding gene: 13507990

Following gene: 13507992

Centisome position: 37.12

GC content: 40.86

Gene sequence:

>1368_bases
ATGTCTGCAACTGCTATTAGCGATCTCTTTGAAAAACCAGCCCAATTTAAAAACAAGAAAATTAAGCTAACGGGTTGATT
GAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGG
TTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTA
GCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTA
TCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTG
CCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGT
CCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGAC
TACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGA
CTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAAC
CTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACT
CGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTT
TGGAATACACCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAAC
TTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAA
CTACCCCAAGGACTTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTC
CCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAA
ATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTT
TGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTA
ACTATTAA

Upstream 100 bases:

>100_bases
AATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCA
AAACCAATCAATAACTTTAT

Downstream 100 bases:

>100_bases
AACTTGACAGAAAAAGTTACCTAATTGGTTAACCATTTACCGCATTTTTATTGCTGTACCTACCATTATTTTTTTGGGAT
TAAATCACTTACTTGGTAGT

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSATAISDLFEKPAQFKNKKIKLTGWLKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPELFALAESISLASAVSVSGTV
ALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRNNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHS
PILTSNDCEGAGETFELKQGKEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFAN
LKDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKALEILKTAQASGQANFEVQDFN
FGLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQNADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQ
IDTQTLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY

Sequences:

>Translated_455_residues
MSATAISDLFEKPAQFKNKKIKLTG*LKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPELFALAESISLASAVSVSGTV
ALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRNNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHS
PILTSNDCEGAGETFELKQGKEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFAN
LKDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKALEILKTAQASGQANFEVQDFN
FGLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQNADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQ
IDTQTLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY
>Mature_454_residues
SATAISDLFEKPAQFKNKKIKLTG*LKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPELFALAESISLASAVSVSGTVA
LTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRNNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSP
ILTSNDCEGAGETFELKQGKEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFANL
KDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKALEILKTAQASGQANFEVQDFNF
GLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQNADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQI
DTQTLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=42.0224719101124, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI4758762, Length=442, Percent_Identity=28.2805429864253, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI45439306, Length=429, Percent_Identity=26.3403263403263, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1787161, Length=466, Percent_Identity=40.5579399141631, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1790571, Length=402, Percent_Identity=24.1293532338308, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556372, Length=418, Percent_Identity=28.2296650717703, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71984122, Length=444, Percent_Identity=27.027027027027, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17551876, Length=421, Percent_Identity=26.1282660332542, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319872, Length=481, Percent_Identity=37.8378378378378, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=459, Percent_Identity=29.6296296296296, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=453, Percent_Identity=26.0485651214128, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922416, Length=443, Percent_Identity=32.5056433408578, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI19921528, Length=443, Percent_Identity=28.8939051918736, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17136276, Length=404, Percent_Identity=27.2277227722772, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 51694; Mature: 51563

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATAISDLFEKPAQFKNKKIKLTGLKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPEL
CCCHHHHHHHHCHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHHCCCCCHH
FALAESISLASAVSVSGTVALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRN
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSPILTSNDCEGAGETFELKQGK
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEECHHHH
EFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFANL
HHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCHHHHHH
KDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
ILKTAQASGQANFEVQDFNFGLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQNA
HHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEECC
DGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQIDTQTLNWYLDMRKWGYFASA
CCCEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHEEEEHHHCCCHHCC
GFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY
CCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SATAISDLFEKPAQFKNKKIKLTGLKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPEL
CCHHHHHHHHCHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHHCCCCCHH
FALAESISLASAVSVSGTVALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRN
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSPILTSNDCEGAGETFELKQGK
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEECHHHH
EFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFANL
HHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCHHHHHH
KDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
ILKTAQASGQANFEVQDFNFGLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQNA
HHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEECC
DGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQIDTQTLNWYLDMRKWGYFASA
CCCEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHEEEEHHHCCCHHCC
GFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY
CCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]