Click here to switch to the map view.

The map label for this gene is fus [H]

Identifier: 13507966

GI number: 13507966

Start: 277660

End: 279726

Strand: Direct

Name: fus [H]

Synonym: MPN227

Alternate gene names: 13507966

Gene position: 277660-279726 (Clockwise)

Preceding gene: 13507965

Following gene: 13507967

Centisome position: 34.01

GC content: 45.86

Gene sequence:

>2067_bases
ATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTC
AGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGG
AGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATT
GACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGA
TGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGACGCCAAGCATCGCGTTACGAAGTGCCCCGGATTATCTTTG
TCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTA
CCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGA
TGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACC
TAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATC
AAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGAT
CAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATG
GTAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTG
GGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAA
GGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTG
CTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAG
TTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAA
GTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAAT
TGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTC
CGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGT
CAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGT
ACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAA
GCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAA
AGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTG
GGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAG
GCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGAT
GCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAG

Upstream 100 bases:

>100_bases
GGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAATA
GAGAATAGAGTTAGTTATTT

Downstream 100 bases:

>100_bases
CAAAATAGATAGGCATAAATCTTTACTTTTATATTTAGTTTTCCCATAAAGTTACGATGCAATACAACATTATTCTTTTA
GTTGATGGTTCGCTCTCGTT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLI
DTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETVWRQASRYEVPRIIFVNKMDKTGANFERSVQSIQQRLGVKAV
PIQLPIGAENDFNGIIDLIEEKVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADI
KRCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISASDDAPFVGLAFKVATDPFV
GRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQ
FAQPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSF
RETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMASGPLAGYPMIDIK
ATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIK
AKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIATKK

Sequences:

>Translated_688_residues
MARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLI
DTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV*RQASRYEVPRIIFVNKMDKTGANFERSVQSIQQRLGVKAV
PIQLPIGAENDFNGIIDLIEEKVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADI
KRCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISASDDAPFVGLAFKVATDPFV
GRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQ
FAQPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSF
RETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMASGPLAGYPMIDIK
ATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIK
AKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIATKK
>Mature_687_residues
ARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLID
TPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV*RQASRYEVPRIIFVNKMDKTGANFERSVQSIQQRLGVKAVP
IQLPIGAENDFNGIIDLIEEKVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADIK
RCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISASDDAPFVGLAFKVATDPFVG
RLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQF
AQPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSFR
ETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMASGPLAGYPMIDIKA
TLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIKA
KVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIATKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=688, Percent_Identity=43.4593023255814, Blast_Score=560, Evalue=1e-159,
Organism=Homo sapiens, GI19923640, Length=707, Percent_Identity=38.048090523338, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=46.0358056265985, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI25306287, Length=284, Percent_Identity=49.2957746478873, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI4503483, Length=476, Percent_Identity=26.2605042016807, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI157426893, Length=150, Percent_Identity=40.6666666666667, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=40, Blast_Score=96, Evalue=2e-19,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=39.8305084745763, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=39.8305084745763, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=39.8305084745763, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1789738, Length=702, Percent_Identity=55.6980056980057, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=485, Percent_Identity=29.2783505154639, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=40, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=41.5094339622642, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17533571, Length=679, Percent_Identity=40.2061855670103, Blast_Score=514, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17556745, Length=713, Percent_Identity=28.7517531556802, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17506493, Length=473, Percent_Identity=25.369978858351, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=146, Percent_Identity=37.6712328767123, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=184, Percent_Identity=32.0652173913043, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=184, Percent_Identity=32.0652173913043, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=687, Percent_Identity=43.2314410480349, Blast_Score=567, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6322359, Length=771, Percent_Identity=31.5175097276265, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324707, Length=827, Percent_Identity=25.8766626360339, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320593, Length=827, Percent_Identity=25.8766626360339, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6323320, Length=118, Percent_Identity=44.9152542372881, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=34.9315068493151, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582462, Length=686, Percent_Identity=43.2944606413994, Blast_Score=561, Evalue=1e-160,
Organism=Drosophila melanogaster, GI221458488, Length=722, Percent_Identity=32.1329639889197, Blast_Score=347, Evalue=2e-95,
Organism=Drosophila melanogaster, GI78706572, Length=152, Percent_Identity=38.1578947368421, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=188, Percent_Identity=36.1702127659575, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585713, Length=188, Percent_Identity=36.1702127659575, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=188, Percent_Identity=36.1702127659575, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=36.231884057971, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76296; Mature: 76165

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERG
CCCEEEEEEECCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCC
ITITSAATSVSWKNCSLNLIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETVR
EEEEECCCEEEECCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QASRYEVPRIIFVNKMDKTGANFERSVQSIQQRLGVKAVPIQLPIGAENDFNGIIDLIEE
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHH
KVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADIK
HEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHH
RCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISA
HHHHHCCCCCEEHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEC
SDDAPFVGLAFKVATDPFVGRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNR
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
NEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQFAQPVISLAVEPKTKADQEKM
HHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEHHHHHHHHHHHHHEECCCCCCHHHHH
SIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSFR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHH
ETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAG
HHCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHH
LENAMASGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPI
HHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCE
MAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIKAKVPLKEMFGYATDLRSFSQG
EEEEEECCHHHHCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
RGNYVMQFSHYAETPKSVVNEIIATKK
CCCEEEEEHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERG
CCEEEEEEECCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCC
ITITSAATSVSWKNCSLNLIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETVR
EEEEECCCEEEECCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QASRYEVPRIIFVNKMDKTGANFERSVQSIQQRLGVKAVPIQLPIGAENDFNGIIDLIEE
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHH
KVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADIK
HEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHH
RCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISA
HHHHHCCCCCEEHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEC
SDDAPFVGLAFKVATDPFVGRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNR
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
NEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQFAQPVISLAVEPKTKADQEKM
HHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEHHHHHHHHHHHHHEECCCCCCHHHHH
SIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSFR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHH
ETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAG
HHCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHH
LENAMASGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPI
HHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCE
MAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIKAKVPLKEMFGYATDLRSFSQG
EEEEEECCHHHHCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
RGNYVMQFSHYAETPKSVVNEIIATKK
CCCEEEEEHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993; 8253680 [H]