Click here to switch to the map view.

The map label for this gene is pth [H]

Identifier: 13507960

GI number: 13507960

Start: 273142

End: 273708

Strand: Direct

Name: pth [H]

Synonym: MPN221

Alternate gene names: 13507960

Gene position: 273142-273708 (Clockwise)

Preceding gene: 13507959

Following gene: 13507961

Centisome position: 33.46

GC content: 39.51

Gene sequence:

>567_bases
ATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAA
GGTAATTGATCGTTTACTAAGTCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAA
AACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGAGCTTGTCAAAACTGA
AACATTAAACCAGATAAGGTAATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGG
TTCAGCCAACAATCATAATGGCATAAAGTCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAA
TTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAA
ACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAA
TGCCTAA

Upstream 100 bases:

>100_bases
TTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTA
AAGGTTAAGCTCCCTGATTA

Downstream 100 bases:

>100_bases
AACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTG
TTGTACACGTTAACTACAAT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVKWACQNW
NIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLE
TALTKAIEALKGYIDGVTMLKLMEVFNA

Sequences:

>Translated_188_residues
MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVK*ACQN*
NIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLE
TALTKAIEALKGYIDGVTMLKLMEVFNA
>Mature_188_residues
MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVK*ACQN*
NIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLE
TALTKAIEALKGYIDGVTMLKLMEVFNA

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=174, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=4e-18,
Organism=Escherichia coli, GI1787455, Length=180, Percent_Identity=35, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21009; Mature: 21009

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLA
CCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEE
KPNTYMNHSGKFVKACQNNIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHL
CCCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
NTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKL
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEVFNA
HHHHCC
>Mature Secondary Structure
MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLA
CCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEE
KPNTYMNHSGKFVKACQNNIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHL
CCCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
NTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKL
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEVFNA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]