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The map label for this gene is yacA [H]

Identifier: 13507961

GI number: 13507961

Start: 273701

End: 274570

Strand: Direct

Name: yacA [H]

Synonym: MPN222

Alternate gene names: 13507961

Gene position: 273701-274570 (Clockwise)

Preceding gene: 13507960

Following gene: 13507962

Centisome position: 33.53

GC content: 42.07

Gene sequence:

>870_bases
ATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGAT
TGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAAC
AGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGAAAGAAAAACTTTCAAGCACAAGCTAGAAAA
ATTCGCTTTGAACAGTTTCAAAAAGCAGCTAGCCTTTATAAAGTAGATAAGCTGCTGTTAGCCCACCACCGCGATGATTT
TATCGAACAGGCCAAAATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATG
GGATGAAGGTCTATCGTCCCTTTATTAAGTACTGAAAAAACGAGATCTTAGCCTTGTGTGAAGAACACAAAGTGCCTTAT
GGTATTGATGAAACCAACGCGCAGCCAATTTACAAACGTAATCAGGTACGCCAGGAGATCGCTAACTGATCGAAGGAAGA
GAAGGAGGAGTTTTACATTGGTGTGTGCGCCATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAA
AACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTT
TTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCA
TTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAG

Upstream 100 bases:

>100_bases
GCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCT
TAAATTAATGGAAGTTTTCA

Downstream 100 bases:

>100_bases
ATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAG
TAATGTAATTAGAGTACCCG

Product: PP-loop superfamily protein ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTVWKKNFQAQARK
IRFEQFQKAASLYKVDKLLLAHHRDDFIEQAKMQLDARKRAMYYGIKTRGELYGMKVYRPFIKYWKNEILALCEEHKVPY
GIDETNAQPIYKRNQVRQEIANWSKEEKEEFYIGVCAMNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSF
LITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS

Sequences:

>Translated_289_residues
MPKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTV*KKNFQAQARK
IRFEQFQKAASLYKVDKLLLAHHRDDFIEQAKMQLDARKRAMYYGIKTRGELYGMKVYRPFIKY*KNEILALCEEHKVPY
GIDETNAQPIYKRNQVRQEIAN*SKEEKEEFYIGVCAMNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSF
LITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS
>Mature_288_residues
PKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTV*KKNFQAQARKI
RFEQFQKAASLYKVDKLLLAHHRDDFIEQAKMQLDARKRAMYYGIKTRGELYGMKVYRPFIKY*KNEILALCEEHKVPYG
IDETNAQPIYKRNQVRQEIAN*SKEEKEEFYIGVCAMNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSFL
ITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 33773; Mature: 33642

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCEEEEEECC
>Mature Secondary Structure 
PKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLI
CCCCEEEECCCCCCHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEE
VHTVPEDTVKKNFQAQARKIRFEQFQKAASLYKVDKLLLAHHRDDFIEQAKMQLDARKRA
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYYGIKTRGELYGMKVYRPFIKYKNEILALCEEHKVPYGIDETNAQPIYKRNQVRQEIAN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
SKEEKEEFYIGVCAMNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSFLIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHH
HHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS
HHCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]