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The map label for this gene is nusA [H]

Identifier: 13507893

GI number: 13507893

Start: 205801

End: 207423

Strand: Direct

Name: nusA [H]

Synonym: MPN154

Alternate gene names: 13507893

Gene position: 205801-207423 (Clockwise)

Preceding gene: 13507892

Following gene: 13507894

Centisome position: 25.21

GC content: 43.01

Gene sequence:

>1623_bases
ATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCAT
CGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGCACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCA
ACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGAC
TTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTC
ACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAA
CGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGT
GGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCT
AGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGACCAATTACGCTTTCCAGGA
GTGATTCGCGTTTACTGGAATTCTTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATG
GAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGG
TCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGAAATGAGGACA
AGTTAAAGTTCTTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACC
AGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAA
CCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCTGCTGAACAAGGGATTGAATTCACCCGGGTCAACT
TTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTC
GCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGA
AGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAA
CCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCA
CCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGAT
TACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCT
TTGATAACTTTGACGATCTCTAG

Upstream 100 bases:

>100_bases
GAAAATGAGTTTGAAGAAATATAATTAAACGATATATTTCTCGTCATTTAAGAGAAGTGTCGCTAGGCACTTCTTTTTTA
TTTGCCCACTTTATCACATT

Downstream 100 bases:

>100_bases
TCGTTAATCTAACCATTTCACACTATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACT
TTTGCGTCTTGTAAAATTAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDD
FLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDG
GLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSMWPITLSRSDSRLLEFLLNSNTPEIANGTIEIKKM
ERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVIWNEDKLKFLVNAVLPAEVVGYNILQDDERDT
SIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQL
ASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPP
PVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL

Sequences:

>Translated_540_residues
MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDD
FLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDG
GLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSM*PITLSRSDSRLLEFLLNSNTPEIANGTIEIKKM
ERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVI*NEDKLKFLVNAVLPAEVVGYNILQDDERDT
SIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQL
ASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPP
PVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL
>Mature_540_residues
MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDD
FLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDG
GLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSM*PITLSRSDSRLLEFLLNSNTPEIANGTIEIKKM
ERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVI*NEDKLKFLVNAVLPAEVVGYNILQDDERDT
SIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQL
ASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPP
PVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=347, Percent_Identity=28.5302593659942, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 59864; Mature: 59864

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCHH
LMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMV
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHH
LLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHH
LSKGEKLDIGKKYLFVIKEIQKQSSMPITLSRSDSRLLEFLLNSNTPEIANGTIEIKKME
HCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCCEEEEEEEC
RSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVINEDKLKFLVNAV
CCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHCCCHHHHCCCEEEEEECCHHHHHHHHHH
LPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDA
CCHHHHCCHHHCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCHH
AEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDL
HHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHCCCCEEEECCCCCCCHHHHHHH
AKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCHH
LMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMV
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHH
LLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHH
LSKGEKLDIGKKYLFVIKEIQKQSSMPITLSRSDSRLLEFLLNSNTPEIANGTIEIKKME
HCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCCEEEEEEEC
RSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVINEDKLKFLVNAV
CCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHCCCHHHHCCCEEEEEECCHHHHHHHHHH
LPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDA
CCHHHHCCHHHCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCHH
AEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDL
HHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHCCCCEEEECCCCCCCHHHHHHH
AKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496 [H]