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The map label for this gene is infB

Identifier: 13507894

GI number: 13507894

Start: 207704

End: 209557

Strand: Direct

Name: infB

Synonym: MPN155

Alternate gene names: 13507894

Gene position: 207704-209557 (Clockwise)

Preceding gene: 13507893

Following gene: 13507895

Centisome position: 25.44

GC content: 45.52

Gene sequence:

>1854_bases
ATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCG
CCAGGTCAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCA
ATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAA
CAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAA
CATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGA
AAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCG
TACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGC
CCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGA
TTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATG
GTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTT
AACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACG
CCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGT
ACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCA
TCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTG
TCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAA
ATTAACGAGGAGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGG
TAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTG
GTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAA
GTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGA
AAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACC
ACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGC
CGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAA
GAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCA
AGTCCAAAAGTTAA

Upstream 100 bases:

>100_bases
CGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTG
TTTAAAGGAAAGGGAAAGCG

Downstream 100 bases:

>100_bases
CGATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTAC
AACGAAACGGTCAAAACCGG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MSKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANIIKHFFLNGVPLTLNSVLSTE
QLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPRPPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGA
YQVKNQEGKTITFIDTPGHEAFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDM
VIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYAIEVQTTKGLGPTATIIVKRG
TLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQISGFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQ
INEELKQKIKSKEIKVLNLIFKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQ
VVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHIAGTLVKTGKVKRGALCKLLR
RNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQVCDIILVYEKQEVKSKS

Sequences:

>Translated_617_residues
MSKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANIIKHFFLNGVPLTLNSVLSTE
QLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPRPPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGA
YQVKNQEGKTITFIDTPGHEAFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDM
VIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYAIEVQTTKGLGPTATIIVKRG
TLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQISGFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQ
INEELKQKIKSKEIKVLNLIFKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQ
VVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHIAGTLVKTGKVKRGALCKLLR
RNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQVCDIILVYEKQEVKSKS
>Mature_616_residues
SKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANIIKHFFLNGVPLTLNSVLSTEQ
LADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPRPPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGAY
QVKNQEGKTITFIDTPGHEAFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDMV
IQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYAIEVQTTKGLGPTATIIVKRGT
LKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQISGFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQI
NEELKQKIKSKEIKVLNLIFKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQV
VKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHIAGTLVKTGKVKRGALCKLLRR
NETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQVCDIILVYEKQEVKSKS

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=552, Percent_Identity=38.2246376811594, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI53729337, Length=552, Percent_Identity=38.2246376811594, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI84043963, Length=518, Percent_Identity=25.6756756756757, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1789559, Length=603, Percent_Identity=41.9568822553897, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI2367247, Length=215, Percent_Identity=32.093023255814, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788922, Length=224, Percent_Identity=29.0178571428571, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI48994988, Length=142, Percent_Identity=33.0985915492958, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=237, Percent_Identity=30.8016877637131, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789737, Length=237, Percent_Identity=30.8016877637131, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=528, Percent_Identity=36.1742424242424, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI212656558, Length=553, Percent_Identity=24.5931283905967, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=246, Percent_Identity=28.4552845528455, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=243, Percent_Identity=30.8641975308642, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=582, Percent_Identity=34.020618556701, Blast_Score=292, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6319282, Length=560, Percent_Identity=25.7142857142857, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI28572034, Length=532, Percent_Identity=34.9624060150376, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24656849, Length=522, Percent_Identity=26.4367816091954, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI160714833, Length=76, Percent_Identity=63.1578947368421, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI78706572, Length=175, Percent_Identity=30.2857142857143, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_MYCPN (P75590)

Other databases:

- EMBL:   U00089
- PIR:   S74002
- RefSeq:   NP_109843.1
- ProteinModelPortal:   P75590
- SMR:   P75590
- GeneID:   877346
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN155
- HOGENOM:   HBG635083
- OMA:   QYGRIRS
- ProtClustDB:   PRK05306
- BioCyc:   MPNE272634:MPN155-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67898; Mature: 67767

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANI
CCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCHHHH
IKHFFLNGVPLTLNSVLSTEQLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPR
HHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCEECCCCCCCCCC
PPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGAYQVKNQEGKTITFIDTPGHE
CCEEEEEECCCCCHHHHHHHHHHHCHHHHHHCCHHHHHCCEEEECCCCCEEEEEECCCCH
AFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDM
HHCCHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
VIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYA
HHHHHHHCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
IEVQTTKGLGPTATIIVKRGTLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQIS
EEEEECCCCCCEEEEEEECCCEEECCEEEECCCCCCEEEECCCCCEEHHHCCCCCCEEEC
GFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQINEELKQKIKSKEIKVLNLI
CEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEE
FKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQ
EEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCEEEEECCCEEEEEECCCHHH
VVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHI
HHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCHHHH
AGTLVKTGKVKRGALCKLLRRNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQ
HHHHHHCCCCCHHHHHHHHHCCCCEECCCHHHHHCCCCCCHHHHCCCCCCEEEECCCCCE
VCDIILVYEKQEVKSKS
EEEEEEEEECHHCCCCC
>Mature Secondary Structure 
SKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANI
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCHHHH
IKHFFLNGVPLTLNSVLSTEQLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPR
HHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCEECCCCCCCCCC
PPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGAYQVKNQEGKTITFIDTPGHE
CCEEEEEECCCCCHHHHHHHHHHHCHHHHHHCCHHHHHCCEEEECCCCCEEEEEECCCCH
AFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDM
HHCCHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
VIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYA
HHHHHHHCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
IEVQTTKGLGPTATIIVKRGTLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQIS
EEEEECCCCCCEEEEEEECCCEEECCEEEECCCCCCEEEECCCCCEEHHHCCCCCCEEEC
GFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQINEELKQKIKSKEIKVLNLI
CEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEE
FKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQ
EEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCEEEEECCCEEEEEECCCHHH
VVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHI
HHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCHHHH
AGTLVKTGKVKRGALCKLLRRNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQ
HHHHHHCCCCCHHHHHHHHHCCCCEECCCHHHHHCCCCCCHHHHCCCCCCEEEECCCCCE
VCDIILVYEKQEVKSKS
EEEEEEEEECHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633