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The map label for this gene is metS [H]

Identifier: 13507762

GI number: 13507762

Start: 28245

End: 29783

Strand: Direct

Name: metS [H]

Synonym: MPN023

Alternate gene names: 13507762

Gene position: 28245-29783 (Clockwise)

Preceding gene: 13507761

Following gene: 13507763

Centisome position: 3.46

GC content: 41.78

Gene sequence:

>1539_bases
ATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTT
AGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACA
AAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATG
TGAAAGCAGTTAGACATTAACTTTGACCACTTTATCCGCACCACTGATCTTAGTCATAAAGCCCAAGTTCAACATGCCTT
TCAACTACTTTATGACAAGGGGTTAATTTACCAAAGTAACTGGGAGGGTGCTTACTGTGTCGAGTGTGAGCAGAACTATT
TCACTTATGATAAACAAACAATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCC
TTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGA
GTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGATTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTAC
CCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAAC
GACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCG
TTTCCACTGCATTTACTGACCAATCTTTCTCCACCAGTTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGA
TTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGT
GTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGA
CATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCG
GCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGAACCAAGCGATGGTTCAGTTT
GACACCGCTTGAAATAACTTCCAGTTTTACAAGGGCATTAATGGCTTACTGCAACTAGTCTTTCAAGCTAGCAAGTTAAT
TGACCAGTTAAAACCGTGGGAGTTAGTCAAACAAACAGATTACACCTTACTCAAGCAATTACTGTTTGCTTGTGTCCGTT
GTACCCAAGTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAATTTCAGT
GCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACCTTT
ATTTAAAAGATTGGATTAA

Upstream 100 bases:

>100_bases
TACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACAC
CTTTTAAAAACACAACTGTA

Downstream 100 bases:

>100_bases
TTAAATTAGATTAGAATTTAATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACC
CAATTTAAGGCGGAACCGTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAKSLGLDPQTFVDQNVAYFQQM
WKQLDINFDHFIRTTDLSHKAQVQHAFQLLYDKGLIYQSNWEGAYCVECEQNYFTYDKQTMLCEIGHQLTLVQEPSLFIA
FKDSKDWIGEMIATNKLNITPESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHN
DPKFNKWWNNNDEEHEVIHLISREITRFHCIYWPIFLHQLGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFG
VDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQANQAMVQF
DTAWNNFQFYKGINGLLQLVFQASKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFS
AQARSKTHLADPQQLQKISLAPVIQPLFKRLD

Sequences:

>Translated_512_residues
MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAKSLGLDPQTFVDQNVAYFQQM
*KQLDINFDHFIRTTDLSHKAQVQHAFQLLYDKGLIYQSNWEGAYCVECEQNYFTYDKQTMLCEIGHQLTLVQEPSLFIA
FKDSKDWIGEMIATNKLNITPESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHN
DPKFNKWWNNNDEEHEVIHLISREITRFHCIY*PIFLHQLGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFG
VDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQANQAMVQF
DTA*NNFQFYKGINGLLQLVFQASKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFS
AQARSKTHLADPQQLQKISLAPVIQPLFKRLD
>Mature_512_residues
MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAKSLGLDPQTFVDQNVAYFQQM
*KQLDINFDHFIRTTDLSHKAQVQHAFQLLYDKGLIYQSNWEGAYCVECEQNYFTYDKQTMLCEIGHQLTLVQEPSLFIA
FKDSKDWIGEMIATNKLNITPESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHN
DPKFNKWWNNNDEEHEVIHLISREITRFHCIY*PIFLHQLGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFG
VDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQANQAMVQF
DTA*NNFQFYKGINGLLQLVFQASKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFS
AQARSKTHLADPQQLQKISLAPVIQPLFKRLD

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=30.9653916211293, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI14043022, Length=516, Percent_Identity=22.8682170542636, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788432, Length=498, Percent_Identity=26.1044176706827, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI212645096, Length=484, Percent_Identity=32.8512396694215, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71992781, Length=377, Percent_Identity=30.7692307692308, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17540794, Length=536, Percent_Identity=22.9477611940299, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321610, Length=530, Percent_Identity=28.8679245283019, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321704, Length=409, Percent_Identity=25.4278728606357, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358483, Length=524, Percent_Identity=28.8167938931298, Blast_Score=198, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24655311, Length=524, Percent_Identity=23.6641221374046, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21355409, Length=124, Percent_Identity=30.6451612903226, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58651; Mature: 58651

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAK
CCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
SLGLDPQTFVDQNVAYFQQMKQLDINFDHFIRTTDLSHKAQVQHAFQLLYDKGLIYQSNW
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCEEECCC
EGAYCVECEQNYFTYDKQTMLCEIGHQLTLVQEPSLFIAFKDSKDWIGEMIATNKLNITP
CCEEEEEECCCCEECCCHHHHHHCCCEEEEEECCCEEEEEECCHHHHHHHHHCCCCCCCC
ESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHND
CHHHHHHHHHHCCCCCCHHEEEECCCEEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCC
PKFNKWWNNNDEEHEVIHLISREITRFHCIYPIFLHQLGFKLPTQFLSHGWIVDGNGHKM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCCHH
SKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHAS
HHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHH
RTFGMIKKYLGGQLDFIEVQDPQVKQLMDQANQAMVQFDTANNFQFYKGINGLLQLVFQA
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
SKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFSAQA
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHH
RSKTHLADPQQLQKISLAPVIQPLFKRLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAK
CCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
SLGLDPQTFVDQNVAYFQQMKQLDINFDHFIRTTDLSHKAQVQHAFQLLYDKGLIYQSNW
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCEEECCC
EGAYCVECEQNYFTYDKQTMLCEIGHQLTLVQEPSLFIAFKDSKDWIGEMIATNKLNITP
CCEEEEEECCCCEECCCHHHHHHCCCEEEEEECCCEEEEEECCHHHHHHHHHCCCCCCCC
ESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHND
CHHHHHHHHHHCCCCCCHHEEEECCCEEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCC
PKFNKWWNNNDEEHEVIHLISREITRFHCIYPIFLHQLGFKLPTQFLSHGWIVDGNGHKM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCCHH
SKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHAS
HHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHH
RTFGMIKKYLGGQLDFIEVQDPQVKQLMDQANQAMVQFDTANNFQFYKGINGLLQLVFQA
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
SKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFSAQA
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHH
RSKTHLADPQQLQKISLAPVIQPLFKRLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496 [H]