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The map label for this gene is gyrA

Identifier: 13507743

GI number: 13507743

Start: 4821

End: 7340

Strand: Direct

Name: gyrA

Synonym: MPN004

Alternate gene names: 13507743

Gene position: 4821-7340 (Clockwise)

Preceding gene: 13507742

Following gene: 13507744

Centisome position: 0.59

GC content: 45.56

Gene sequence:

>2520_bases
ATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAG
TGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTA
AACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGG
ATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGA
CTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTT
ATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGACATTGACAAGGACACGGTCGACTTTGTCGCTAAC
TATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGC
GGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTG
ACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGG
ATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGC
TGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTG
AGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGAC
ACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACT
GGTTAAGGGTGCACCTAAATTGCTTAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTC
GCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATT
GATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGA
TGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAAC
AACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAG
CAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGA
TGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACGCTT
ATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTC
TGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGG
TTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGG
TGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCC
AACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCA
AGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTG
CCATGGGGGTCTTGGGGATTAATCTCAACCAGTGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTA
CTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGT
TAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGA
TTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGCGAAAAAACACTTCTGGTGTTAAACTA
ATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAAC
CGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAA

Upstream 100 bases:

>100_bases
TTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAAC
GTAAAGAACATTGATATCTA

Downstream 100 bases:

>100_bases
CCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAG
CAATGCGCAAACTGTTGCAC

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 839; Mature: 838

Protein sequence:

>839_residues
MAKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYTGGMHHDRPFKKSAR
IVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVAN
YDGEEQEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKG
IESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIADIRDESSREGIRLVIEVKRD
TVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQALTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNI
DEVIAIIKKSANNQVAMEALHERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKE
QLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYTDDSISQLLV
CSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLIKIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFA
NILSIGKRAILFKENDVLFSVIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQCEFVNGLSTSSNGSLL
LSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKIVRISLEQLSEQRKNTSGVKL
IKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ

Sequences:

>Translated_839_residues
MAKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYTGGMHHDRPFKKSAR
IVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVAN
YDGEEQEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKG
IESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIADIRDESSREGIRLVIEVKRD
TVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQALTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNI
DEVIAIIKKSANNQVAMEALHERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKE
QLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYTDDSISQLLV
CSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLIKIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFA
NILSIGKRAILFKENDVLFSVIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQCEFVNGLSTSSNGSLL
LSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKIVRISLEQLSEQRKNTSGVKL
IKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ
>Mature_838_residues
AKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYTGGMHHDRPFKKSARI
VGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVANY
DGEEQEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKGI
ESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIADIRDESSREGIRLVIEVKRDT
VPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQALTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNID
EVIAIIKKSANNQVAMEALHERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKEQ
LTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYTDDSISQLLVC
STHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLIKIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFAN
ILSIGKRAILFKENDVLFSVIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQCEFVNGLSTSSNGSLLL
SVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKIVRISLEQLSEQRKNTSGVKLI
KLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family

Homologues:

Organism=Homo sapiens, GI19913406, Length=362, Percent_Identity=28.4530386740331, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI19913408, Length=440, Percent_Identity=24.5454545454545, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1788562, Length=854, Percent_Identity=44.847775175644, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=708, Percent_Identity=35.3107344632768, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI212645657, Length=217, Percent_Identity=28.110599078341, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17535065, Length=173, Percent_Identity=31.2138728323699, Blast_Score=72, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=222, Percent_Identity=27.4774774774775, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17136538, Length=285, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRA_MYCPN (P22446)

Other databases:

- EMBL:   U00089
- EMBL:   X53555
- PIR:   S73476
- RefSeq:   NP_109692.1
- ProteinModelPortal:   P22446
- IntAct:   P22446
- GeneID:   877234
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN004
- HOGENOM:   HBG635183
- OMA:   NTHDILL
- ProtClustDB:   PRK05560
- BioCyc:   MPNE272634:MPN004-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760
- Gene3D:   G3DSA:3.30.1360.40
- Gene3D:   G3DSA:3.90.199.10
- SMART:   SM00434
- TIGRFAMs:   TIGR01063

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 93358; Mature: 93227

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LYGAYTGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGN
HHEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEECCCC
FGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVANYDGEEQEPTVLPAAFPNLLA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEECHHHHHHHHC
NGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKG
CCCCCEEEEEECCCCCCCHHHHHHCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEECC
IESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIA
HHHHHHCCCCCEEEEECCCHHHCCCCCEEEEECCCHHHCHHHHHHHHHHHHHHHHCCCHH
DIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQA
HCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEECHHHHHHHCCCHHHHHHHHH
LTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNIDEVIAIIKKSANNQVAMEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
HERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKE
HHHHCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDAYNLQHRG
HHHHHHHHHCCCCHHHEEEECCCCCCCCCCEECCCEEEEEECCCEEEEECCCEECCCCCC
GVGVKGLTTYTDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLI
CCCCCCEEECCCCCHHHEEEEECCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEE
KIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFANILSIGKRAILFKENDVLFS
EECCCCCEEEEEEECCCCCCEEEEEECCCCEEECCHHHHHHHHHHCCEEEEEECCCCEEE
VIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQCEFVNGLSTSSNGSLL
EECCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEE
LSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKI
EEECCCCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCEEEEECCCCE
VRISLEQLSEQRKNTSGVKLIKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ
EEEEHHHHHHHHCCCCCEEEEEECCHHCCCEEEEEECCHHHEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRV
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LYGAYTGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGN
HHEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEECCCC
FGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVANYDGEEQEPTVLPAAFPNLLA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEECHHHHHHHHC
NGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKG
CCCCCEEEEEECCCCCCCHHHHHHCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEECC
IESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIA
HHHHHHCCCCCEEEEECCCHHHCCCCCEEEEECCCHHHCHHHHHHHHHHHHHHHHCCCHH
DIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQA
HCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEECHHHHHHHCCCHHHHHHHHH
LTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNIDEVIAIIKKSANNQVAMEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
HERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKE
HHHHCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDAYNLQHRG
HHHHHHHHHCCCCHHHEEEECCCCCCCCCCEECCCEEEEEECCCEEEEECCCEECCCCCC
GVGVKGLTTYTDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLI
CCCCCCEEECCCCCHHHEEEEECCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEE
KIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFANILSIGKRAILFKENDVLFS
EECCCCCEEEEEEECCCCCCEEEEEECCCCEEECCHHHHHHHHHHCCEEEEEECCCCEEE
VIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQCEFVNGLSTSSNGSLL
EECCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEE
LSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKI
EEECCCCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCEEEEECCCCE
VRISLEQLSEQRKNTSGVKLIKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ
EEEEHHHHHHHHCCCCCEEEEEECCHHCCCEEEEEECCHHHEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 2172693; 11271496