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The map label for this gene is vhuA

Identifier: 298575368

GI number: 298575368

Start: 1133010

End: 1134266

Strand: Direct

Name: vhuA

Synonym: NA

Alternate gene names: 298575368

Gene position: 1133010-1134266 (Clockwise)

Preceding gene: 15669379

Following gene: 15669380

Centisome position: 68.05

GC content: 37.79

Gene sequence:

>1257_bases
ATGGGGAAGATAGTAATTGAGCCCCTATCAAGATTAGAAGGGCATGGTAAAGTTACAATAACCTTAGATGAAAATGGAAA
ACCTAAGGATGTTAAGTTGCATATAACTGCATTGAGAGGATTTGAGCAGTTCGTTGTTGGAAGACCTGCTGAAGAAGTTC
CAAGAATTGTCCCAAGAATCTGTGGTATCTGCCAAACAGCCCACCACTTAGCAAGTGTTAAGGCAATTGATGCCGCTTGG
GGTGTAGAGATTCCAGAACCAGCTAAGAAATTGAGAGAGTTAATGCACATAGGAAATATGATTCATAGCCATGCATTGCA
CTTTTACTTCTTAGCAGCTCCTGACTTTGTTCTTGGTCCAGATGCAGACCCAGCAATAAGAAACATTGTGGGAGTTATAG
ATAAAGCTCCAGACGTTGCTAAGCAAGCTATCGCCTTAAGAAAGTTTGGGCAAAAGATTGTTGAAGCAGTTGGAGGAAAA
GCTATTCATCCAGTTACTGGAATTCCTGGTGGGCAAGCAAAGAGATTAACTGAAGAAGAAAGAGATGAGTTATTAAAAGA
TGCTGACCAGATGATAGAATACGCTAAAAATGGTGTTGAGCTTATAAAACAATTAAATGAGCAATATATGGAACAGATAA
AGACATTAGGAGTTATTGACACCTATTATTTAGGTTTGGTTAAAGATGGAAAACATAACTTCTATGACGATACCTTAAGA
TTCTTATCTCCAGATGGAAAAGAAAAAGTTGAGTTTAAACCGGAAGAATACTTGAACTATATTGGGGAATATGTAGTTCC
ATACAACTATGTAAAACACCCTTACTACAAAAAAGTCGGTTATCCTGAAGGAGTTTATAGGGTTGGGCCATTAGCGATGC
TAAACGTTTGTGATGAAATGGAGACCCCGCTTGCAGAAGAATACAGAAAAGAATTTTTAGAGATCTTTGGATTTCCAGCA
AATCAGTCATTGGCATATAATCATGCAAGGTTGATTGAGTTAGTTGAAGCATGTGAAAAGGCAAAAATATTATTGGAAGA
TAATGATATTACATCAGATGACATTAAGGCAGATGTTGAACCAAAGGCTGGAAATGGTGTTGGAGTAGTTTATGCTCCAA
GAGGAGTTTTAATTCACAACTATGAAACAGATGAGAATGGAATCGTTGTTAAGGCAAACATGATTGTCGCTACAACACAC
AACGTTCCAACAATGGAAAAGGCTATCCAACAGGCAGCTCAAGTAATCTTCAAATAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: methylviologen-reducing hydrogenase subunit alpha

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:
NA

Sequences:

>Translated_418_residues
MGKIVIEPLSRLEGHGKVTITLDENGKPKDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAIDAAW
GVEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGPDADPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGK
AIHPVTGIPGGQAKRLTEEERDELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLR
FLSPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEMETPLAEEYRKEFLEIFGFPA
NQSLAYNHARLIELVEACEKAKILLEDNDITSDDIKADVEPKAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTH
NVPTMEKAIQQAAQVIFK
>Mature_417_residues
GKIVIEPLSRLEGHGKVTITLDENGKPKDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAIDAAWG
VEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGPDADPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGKA
IHPVTGIPGGQAKRLTEEERDELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLRF
LSPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEMETPLAEEYRKEFLEIFGFPAN
QSLAYNHARLIELVEACEKAKILLEDNDITSDDIKADVEPKAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTHN
VPTMEKAIQQAAQVIFK

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family

Homologues:

Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=36.5217391304348, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787207, Length=115, Percent_Identity=33.9130434782609, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VHUA_METJA (Q58592)

Other databases:

- EMBL:   L77117
- PIR:   G64448
- RefSeq:   NP_248187.2
- ProteinModelPortal:   Q58592
- GeneID:   1452611
- GenomeReviews:   L77117_GR
- TIGR:   MJ1192
- HOGENOM:   HBG287963
- OMA:   VPRIVPR
- ProtClustDB:   CLSK2754647
- BioCyc:   MJAN243232:MJ_1192-MONOMER
- InterPro:   IPR001501
- InterPro:   IPR018194

Pfam domain/function: PF00374 NiFeSe_Hases

EC number: 1.12.98.1

Molecular weight: Translated: 46652; Mature: 46521

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00507 NI_HGENASE_L_1; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIVIEPLSRLEGHGKVTITLDENGKPKDVKLHITALRGFEQFVVGRPAEEVPRIVPRI
CCCEEECHHHHCCCCCEEEEEECCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHH
CGICQTAHHLASVKAIDAAWGVEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECC
DADPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGKAIHPVTGIPGGQAKRLTEEE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHCCHHH
RDELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCHHHHHHH
FLSPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEM
HCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCCHHCCHHHHHHHHHHH
ETPLAEEYRKEFLEIFGFPANQSLAYNHARLIELVEACEKAKILLEDNDITSDDIKADVE
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCC
PKAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTHNVPTMEKAIQQAAQVIFK
CCCCCCEEEEEECCCEEEECCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKIVIEPLSRLEGHGKVTITLDENGKPKDVKLHITALRGFEQFVVGRPAEEVPRIVPRI
CCEEECHHHHCCCCCEEEEEECCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHH
CGICQTAHHLASVKAIDAAWGVEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECC
DADPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGKAIHPVTGIPGGQAKRLTEEE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHCCHHH
RDELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCHHHHHHH
FLSPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEM
HCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCCHHCCHHHHHHHHHHH
ETPLAEEYRKEFLEIFGFPANQSLAYNHARLIELVEACEKAKILLEDNDITSDDIKADVE
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCC
PKAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTHNVPTMEKAIQQAAQVIFK
CCCCCCEEEEEECCCEEEECCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087