Click here to switch to the map view.

The map label for this gene is truD

Identifier: 161579521

GI number: 161579521

Start: 1313349

End: 1314617

Strand: Direct

Name: truD

Synonym: MJ1364

Alternate gene names: 161579521

Gene position: 1313349-1314617 (Clockwise)

Preceding gene: 15669551

Following gene: 15669555

Centisome position: 78.88

GC content: 26.48

Gene sequence:

>1269_bases
ATGAAAAATATTTCAACCAAAGAGTCTTTTTTTAAATTTAAGTTTAATAATAGAAGTTTATATTGTTGGGGTGCTTTTAT
GAAACTTAGAATGAAGCCAGAGGATTTTATCGTTGAAGAAATTATTGATTTTAATAAAATAGCTGGAGATAGATGCTACC
TTTATAAATTAACAAAGAGAAATATAGAGAGTTTGAAAGCATTCTCTTATATTGCAAAAAAATTTAAAATTCCATTGAAG
GATATTGGATACTGTGGATTAAAAGATAGGCATGCTTTAACTACTCAATATATATCTATACCAAAAAAATATGGAAAATT
AAGCTTAGATGAACCAAATTTAAAATTAGAGTTAATCGGAGAATCAAAATTTCTATTGTTGGGAGATTTAGAGGGAAATA
GATTCACAATAACAGTTAGGGGTTTAAAAAAAGAAGACATCCCAAAAATAAAAGAAAATTTAAAATACTTAGAATTTGGA
GCTCCAAATTATTTTGATAGTCAGAGATTTGGAAGCGTTTTTGATAAAAAATTCATTGCCAAAGAAGTTATAAAAGGCAA
TTATGAAGAAGCTGTGAAAATATTGCTAACGAAATATAAAAAATCTGAAAAGAAATTAATAAAAGATTTAAAGAGATTTA
TAGATAAAAACTGGGGAGATTGGGATAAAATTTGGGAGTATATTAAGGAAAATAATATAAAGTCAAGATTATATGTGAAT
ATGGTTAAAGAGCTTAAAAAAAGCAATGATTATAAAAAAGCTTTAAGTTATGTTGATGATAGGCTGAAGAAAATTTTTGT
TGCTGCTTATCAAAGCTATCTATGGAATGAGTGTGTAAAAGAGTTATTAAGAAAATATGTTCCAGAAGAAGATAGGGTTT
ATTATGAATATGAATGTGGAACCCTTATGTTCTATAAAAAAATGGATGAGGAAGTGTTTAATATATTGAAAGATAAAAAA
TTTCCAACAATAGCTCCAGACATTGAATATAGTGGAGAAGAAAAAGAAATTATTGAAGAAATCTTAAAGAGAGAAGGCCT
TACTATGGAAGAGCTAAATAATATAGGAGAGTTAGGGAAGTTCATATACAGTGAAAGAAAGATTCTTTCAATCCCTAAAA
ACTTAAAAATTGGAGAGTTTGAAGAGGATGAATTGAATAAAGGAAAATATAAAATAACTTTAAGCTATGAATTGGAAAAA
GGAAGTTATGCAACAATTATAATAAAAAGGGCTTTCTTAGGTGTAAAAACTAAAAAAAGAAAAAGATAA

Upstream 100 bases:

>100_bases
GTCATCTATTTTAGAGTTAAAAAATTACCCAAGAAAGTTAAAAATTCCTTTAGGTTATTTTTTCTGTGGAACTAATTCCT
GTGAATTTTCAAGTCTTTTT

Downstream 100 bases:

>100_bases
ATTAAACAATTAATAGTTAGTGTTAAATATTTAAAGGCACAATTTAATAATAATTTCTACAAAAATATCAACGACACTTT
TTACAAATAGATGGTGATTA

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D 2; tRNA-uridine isomerase D 2

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKNISTKESFFKFKFNNRSLYCWGAFMKLRMKPEDFIVEEIIDFNKIAGDRCYLYKLTKRNIESLKAFSYIAKKFKIPLK
DIGYCGLKDRHALTTQYISIPKKYGKLSLDEPNLKLELIGESKFLLLGDLEGNRFTITVRGLKKEDIPKIKENLKYLEFG
APNYFDSQRFGSVFDKKFIAKEVIKGNYEEAVKILLTKYKKSEKKLIKDLKRFIDKNWGDWDKIWEYIKENNIKSRLYVN
MVKELKKSNDYKKALSYVDDRLKKIFVAAYQSYLWNECVKELLRKYVPEEDRVYYEYECGTLMFYKKMDEEVFNILKDKK
FPTIAPDIEYSGEEKEIIEEILKREGLTMEELNNIGELGKFIYSERKILSIPKNLKIGEFEEDELNKGKYKITLSYELEK
GSYATIIIKRAFLGVKTKKRKR

Sequences:

>Translated_422_residues
MKNISTKESFFKFKFNNRSLYCWGAFMKLRMKPEDFIVEEIIDFNKIAGDRCYLYKLTKRNIESLKAFSYIAKKFKIPLK
DIGYCGLKDRHALTTQYISIPKKYGKLSLDEPNLKLELIGESKFLLLGDLEGNRFTITVRGLKKEDIPKIKENLKYLEFG
APNYFDSQRFGSVFDKKFIAKEVIKGNYEEAVKILLTKYKKSEKKLIKDLKRFIDKNWGDWDKIWEYIKENNIKSRLYVN
MVKELKKSNDYKKALSYVDDRLKKIFVAAYQSYLWNECVKELLRKYVPEEDRVYYEYECGTLMFYKKMDEEVFNILKDKK
FPTIAPDIEYSGEEKEIIEEILKREGLTMEELNNIGELGKFIYSERKILSIPKNLKIGEFEEDELNKGKYKITLSYELEK
GSYATIIIKRAFLGVKTKKRKR
>Mature_422_residues
MKNISTKESFFKFKFNNRSLYCWGAFMKLRMKPEDFIVEEIIDFNKIAGDRCYLYKLTKRNIESLKAFSYIAKKFKIPLK
DIGYCGLKDRHALTTQYISIPKKYGKLSLDEPNLKLELIGESKFLLLGDLEGNRFTITVRGLKKEDIPKIKENLKYLEFG
APNYFDSQRFGSVFDKKFIAKEVIKGNYEEAVKILLTKYKKSEKKLIKDLKRFIDKNWGDWDKIWEYIKENNIKSRLYVN
MVKELKKSNDYKKALSYVDDRLKKIFVAAYQSYLWNECVKELLRKYVPEEDRVYYEYECGTLMFYKKMDEEVFNILKDKK
FPTIAPDIEYSGEEKEIIEEILKREGLTMEELNNIGELGKFIYSERKILSIPKNLKIGEFEEDELNKGKYKITLSYELEK
GSYATIIIKRAFLGVKTKKRKR

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI50727002, Length=400, Percent_Identity=21.75, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789102, Length=155, Percent_Identity=30.3225806451613, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557232, Length=391, Percent_Identity=24.0409207161125, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324817, Length=247, Percent_Identity=31.1740890688259, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355799, Length=254, Percent_Identity=27.5590551181102, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD2_METJA (Q58759)

Other databases:

- EMBL:   L77117
- PIR:   C64470
- RefSeq:   NP_248367.2
- ProteinModelPortal:   Q58759
- SMR:   Q58759
- GeneID:   1452266
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1364
- NMPDR:   fig|243232.1.peg.1404
- TIGR:   MJ1364
- HOGENOM:   HBG712968
- OMA:   IGYCGLK
- ProtClustDB:   PRK00984
- BioCyc:   MJAN243232:MJ_1364-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 50180; Mature: 50180

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 89-89

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNISTKESFFKFKFNNRSLYCWGAFMKLRMKPEDFIVEEIIDFNKIAGDRCYLYKLTKR
CCCCCCCCCEEEEEECCCEEEEEEEEHHEECCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
NIESLKAFSYIAKKFKIPLKDIGYCGLKDRHALTTQYISIPKKYGKLSLDEPNLKLELIG
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCEEEEEC
ESKFLLLGDLEGNRFTITVRGLKKEDIPKIKENLKYLEFGAPNYFDSQRFGSVFDKKFIA
CCCEEEEEECCCCEEEEEEECCCCCCCHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHH
KEVIKGNYEEAVKILLTKYKKSEKKLIKDLKRFIDKNWGDWDKIWEYIKENNIKSRLYVN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
MVKELKKSNDYKKALSYVDDRLKKIFVAAYQSYLWNECVKELLRKYVPEEDRVYYEYECG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
TLMFYKKMDEEVFNILKDKKFPTIAPDIEYSGEEKEIIEEILKREGLTMEELNNIGELGK
EEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
FIYSERKILSIPKNLKIGEFEEDELNKGKYKITLSYELEKGSYATIIIKRAFLGVKTKKR
HHHCCCHHEECCCCCCCCCCCHHHCCCCEEEEEEEEEECCCCEEEEEEEHHHHCCCCCCC
KR
CC
>Mature Secondary Structure
MKNISTKESFFKFKFNNRSLYCWGAFMKLRMKPEDFIVEEIIDFNKIAGDRCYLYKLTKR
CCCCCCCCCEEEEEECCCEEEEEEEEHHEECCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
NIESLKAFSYIAKKFKIPLKDIGYCGLKDRHALTTQYISIPKKYGKLSLDEPNLKLELIG
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCEEEEEC
ESKFLLLGDLEGNRFTITVRGLKKEDIPKIKENLKYLEFGAPNYFDSQRFGSVFDKKFIA
CCCEEEEEECCCCEEEEEEECCCCCCCHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHH
KEVIKGNYEEAVKILLTKYKKSEKKLIKDLKRFIDKNWGDWDKIWEYIKENNIKSRLYVN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
MVKELKKSNDYKKALSYVDDRLKKIFVAAYQSYLWNECVKELLRKYVPEEDRVYYEYECG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
TLMFYKKMDEEVFNILKDKKFPTIAPDIEYSGEEKEIIEEILKREGLTMEELNNIGELGK
EEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
FIYSERKILSIPKNLKIGEFEEDELNKGKYKITLSYELEKGSYATIIIKRAFLGVKTKKR
HHHCCCHHEECCCCCCCCCCCHHHCCCCEEEEEEEEEECCCCEEEEEEEHHHHCCCCCCC
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087