Click here to switch to the map view.

The map label for this gene is topA

Identifier: 15669848

GI number: 15669848

Start: 1633490

End: 1635775

Strand: Direct

Name: topA

Synonym: MJ1652

Alternate gene names: 15669848

Gene position: 1633490-1635775 (Clockwise)

Preceding gene: 15669844

Following gene: 15669849

Centisome position: 98.11

GC content: 32.24

Gene sequence:

>2286_bases
GTGGCTGAAATTATGACAGCGTTAATAATCTGTGAAAAACCAAGTGTTGCCAAAAAGATAGCAAACGCTTTAGGAAAAGC
TAAGAAAAAAAGCATTGATGGAGTTCCATACTATGAATTAGAAAGAGATGGGAAAAAAATTATAGTAGCAAGTGCTGTTG
GGCATCTATTTACCTTAGTTGAAAAAGAGAATAAAGAATTTGGCTTTTATCCAGTATTTGATATAAAATGGGTGCCTGCA
AGTGTTGATAAGGGAAAAGAGTATGTAAATAAATACATAAAGGCATTAAAAAAACTATCAAAAGATGCAGATGAGTTCTA
TATAGCAACAGATTGGGACATTGAAGGGGAGCTAATAGGTTATCACGCATTAAAATATTGTTGTGGAAGAGAAAAGGCAA
AGAGAATGAGATTCTCATCATTAACAAAAAAAGAGATAGTTAGAGCATTTGAAAATCCGGATGAGATTGATTACGGCTTA
GTTGATGCAGGAGAGAGCAGGCATATTTTAGATTGGTATTTTGGTATAAACCTATCGAGAGCTTTGATGAACGCCATAAG
GGCAGTGAATAGATGGAAAACGATGAGTGTTGGAAGAGTTCAAGGTCCTGCATTAGCTTTTTTAACTGAAAGAGAATTGG
AAATTAAAAAATTTATCCCAAAGCCGTATTGGGTTATTGAGGCACTATTAAAAGATAATTTAAAGGCAATACACGAGAAA
GAGAAATTTTGGAATGAAAAAGAGGCAAAAAATGTTTATGAAAAAATAAAAGATGAAAAATCTGCCAAAGTTGTTGAAAT
AAAGAAAACAAAAAGAAAGTTAAAGCCACTTCCACCTTTTGACTTAGGAACTCTACAGAGAGAGGCATATAGTTATTTTA
AAATATCTCCAAAGGAAACGCAAGAAATTGCCCAAAAGCTTTATGAGAATGCTCTAATTAGCTATCCGAGAACATCCAGC
CAAAAACTTCCAAAAGATAGAAAGTATTTGGAAGATATTCTAAATATAATAAAAAATCATCCAGTTTATGGAAAATGGGC
AGAGAGAATTTTAAAAGAGAATTTAAAACCAGTTGAAGGAAAGAAAGAAGACCCTGCACATCCAGCTATACACATTGTAG
ATATTCCAAAGGAAGAACTTTCAGAAAAAGAGAAGGAGATTTATGATTTAATTGCAAGAAGAACTTTAGCTGCTTTTTGG
GATAATGCAGAGAGGGAATATTTAAATGTAAAAATTGACATTAAAGGAGAGAAGTTTAAGTTATCTGGTTCAAGGACAGT
AAAAGAAGGATGGCATGAAATTTATTACTTCCCAAAATTTGATGAGATTGAATTGCCCCCATTAAAGAAAAATGACATAA
TTAAAGTTGAAAAGATAACAATAACAAGAAAAGAAACGCAACCACCAAAAAGATACACTGTTGCAAGCATAATTAAGGAA
TTAGAGAAAAGAGGGCTGGGAACAAAAGCCACTAGGGCAGAGATCATAGACAAATTAATAAAAAGGGGCTATGTCATTGA
TGATGGTTCTTTAAAAGTAACTGACTTAGGAATTTCAGTAATCGAGACATTAAAAAGATTCTGCCCAGAAATTATCGATG
AAAAGATGACAAGGGATTTAGAGGAAAAGTTGGAGAAAATACAGTTTAGAAAAATTAAAAAGGATGATGTTTTAGATGAG
GCTGAAAAAAGATTAAGAAAAATATTGGAAGAATTTAAAAAGAAAGAGGAAGATATTGGAATCTACCTTATCAAAAACTT
AGATGCCACAAATAAAAAAGCAAAAATTGTTGGAAAATGTCCTAAATGTGGAGGAGACTTAATTTTAATAAGACATAAAA
AGGGCAGGTTTGTTGGCTGCTCTAACTATCCTGAATGTGATGTAAAATACTCCTTACCAGATAAAGGTAGGATAAAAATT
CCAAACAAAGTTTGTGATGCATGTAAATCACCTATTTTAAAAATAGGAGATAGAGAGATATGCATAAATCCAGAATGTCC
TTTAAAACAAGTAGAGGTTAAAGAAGAAGATAGGATTTGCCCAAAGTGCGGAGCTAAGCTAATTTTAAAGAAAGGAGTTT
ATGGAGCATTTTATGGTTGTTCAAACTATCCAAAGTGTAAATATACAGAACCAATAAATAAAAAAGAAGTTGTAGGAAAA
TGCCCTAAATGTGGAGGAGATTTAGTTGTTAGAGAAGGTAAGTTTGGAAAGTTTGTTGGTTGCTCTAACTATCCAAAGTG
TAGATATACTGAAAAACTAAAACTAAATGAAAAAGAAGAAAAATAA

Upstream 100 bases:

>100_bases
ATCATCCCTCATATAAATCCCCAAAAATATTTATTTACCACAACTACAACTAATTACAAAGAAATCATATATAAATTATA
ATATATTCAGTTAGATACGC

Downstream 100 bases:

>100_bases
AATGGTGAATTAGCTATGACTACAAAGCTATATGTTGATTTTGGAGGATATTCTGCTATAGAAAAAGGAAATTTAATCAT
TCCAAGGGATAATATTTTAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MAEIMTALIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGKKIIVASAVGHLFTLVEKENKEFGFYPVFDIKWVPA
SVDKGKEYVNKYIKALKKLSKDADEFYIATDWDIEGELIGYHALKYCCGREKAKRMRFSSLTKKEIVRAFENPDEIDYGL
VDAGESRHILDWYFGINLSRALMNAIRAVNRWKTMSVGRVQGPALAFLTERELEIKKFIPKPYWVIEALLKDNLKAIHEK
EKFWNEKEAKNVYEKIKDEKSAKVVEIKKTKRKLKPLPPFDLGTLQREAYSYFKISPKETQEIAQKLYENALISYPRTSS
QKLPKDRKYLEDILNIIKNHPVYGKWAERILKENLKPVEGKKEDPAHPAIHIVDIPKEELSEKEKEIYDLIARRTLAAFW
DNAEREYLNVKIDIKGEKFKLSGSRTVKEGWHEIYYFPKFDEIELPPLKKNDIIKVEKITITRKETQPPKRYTVASIIKE
LEKRGLGTKATRAEIIDKLIKRGYVIDDGSLKVTDLGISVIETLKRFCPEIIDEKMTRDLEEKLEKIQFRKIKKDDVLDE
AEKRLRKILEEFKKKEEDIGIYLIKNLDATNKKAKIVGKCPKCGGDLILIRHKKGRFVGCSNYPECDVKYSLPDKGRIKI
PNKVCDACKSPILKIGDREICINPECPLKQVEVKEEDRICPKCGAKLILKKGVYGAFYGCSNYPKCKYTEPINKKEVVGK
CPKCGGDLVVREGKFGKFVGCSNYPKCRYTEKLKLNEKEEK

Sequences:

>Translated_761_residues
MAEIMTALIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGKKIIVASAVGHLFTLVEKENKEFGFYPVFDIKWVPA
SVDKGKEYVNKYIKALKKLSKDADEFYIATDWDIEGELIGYHALKYCCGREKAKRMRFSSLTKKEIVRAFENPDEIDYGL
VDAGESRHILDWYFGINLSRALMNAIRAVNRWKTMSVGRVQGPALAFLTERELEIKKFIPKPYWVIEALLKDNLKAIHEK
EKFWNEKEAKNVYEKIKDEKSAKVVEIKKTKRKLKPLPPFDLGTLQREAYSYFKISPKETQEIAQKLYENALISYPRTSS
QKLPKDRKYLEDILNIIKNHPVYGKWAERILKENLKPVEGKKEDPAHPAIHIVDIPKEELSEKEKEIYDLIARRTLAAFW
DNAEREYLNVKIDIKGEKFKLSGSRTVKEGWHEIYYFPKFDEIELPPLKKNDIIKVEKITITRKETQPPKRYTVASIIKE
LEKRGLGTKATRAEIIDKLIKRGYVIDDGSLKVTDLGISVIETLKRFCPEIIDEKMTRDLEEKLEKIQFRKIKKDDVLDE
AEKRLRKILEEFKKKEEDIGIYLIKNLDATNKKAKIVGKCPKCGGDLILIRHKKGRFVGCSNYPECDVKYSLPDKGRIKI
PNKVCDACKSPILKIGDREICINPECPLKQVEVKEEDRICPKCGAKLILKKGVYGAFYGCSNYPKCKYTEPINKKEVVGK
CPKCGGDLVVREGKFGKFVGCSNYPKCRYTEKLKLNEKEEK
>Mature_760_residues
AEIMTALIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGKKIIVASAVGHLFTLVEKENKEFGFYPVFDIKWVPAS
VDKGKEYVNKYIKALKKLSKDADEFYIATDWDIEGELIGYHALKYCCGREKAKRMRFSSLTKKEIVRAFENPDEIDYGLV
DAGESRHILDWYFGINLSRALMNAIRAVNRWKTMSVGRVQGPALAFLTERELEIKKFIPKPYWVIEALLKDNLKAIHEKE
KFWNEKEAKNVYEKIKDEKSAKVVEIKKTKRKLKPLPPFDLGTLQREAYSYFKISPKETQEIAQKLYENALISYPRTSSQ
KLPKDRKYLEDILNIIKNHPVYGKWAERILKENLKPVEGKKEDPAHPAIHIVDIPKEELSEKEKEIYDLIARRTLAAFWD
NAEREYLNVKIDIKGEKFKLSGSRTVKEGWHEIYYFPKFDEIELPPLKKNDIIKVEKITITRKETQPPKRYTVASIIKEL
EKRGLGTKATRAEIIDKLIKRGYVIDDGSLKVTDLGISVIETLKRFCPEIIDEKMTRDLEEKLEKIQFRKIKKDDVLDEA
EKRLRKILEEFKKKEEDIGIYLIKNLDATNKKAKIVGKCPKCGGDLILIRHKKGRFVGCSNYPECDVKYSLPDKGRIKIP
NKVCDACKSPILKIGDREICINPECPLKQVEVKEEDRICPKCGAKLILKKGVYGAFYGCSNYPKCKYTEPINKKEVVGKC
PKCGGDLVVREGKFGKFVGCSNYPKCRYTEKLKLNEKEEK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI10835218, Length=709, Percent_Identity=27.6445698166432, Blast_Score=206, Evalue=9e-53,
Organism=Homo sapiens, GI4507635, Length=774, Percent_Identity=24.6770025839793, Blast_Score=180, Evalue=4e-45,
Organism=Escherichia coli, GI1787529, Length=703, Percent_Identity=28.4495021337127, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI1788061, Length=624, Percent_Identity=27.2435897435897, Blast_Score=175, Evalue=1e-44,
Organism=Escherichia coli, GI48994931, Length=158, Percent_Identity=36.7088607594937, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=628, Percent_Identity=28.0254777070064, Blast_Score=203, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI32563869, Length=793, Percent_Identity=25.3467843631778, Blast_Score=172, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323263, Length=639, Percent_Identity=25.9780907668232, Blast_Score=180, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24585251, Length=696, Percent_Identity=25.2873563218391, Blast_Score=188, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24640096, Length=754, Percent_Identity=23.474801061008, Blast_Score=163, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_METJA (Q59046)

Other databases:

- EMBL:   L77117
- PIR:   B64506
- RefSeq:   NP_248662.1
- ProteinModelPortal:   Q59046
- GeneID:   1452561
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1652
- NMPDR:   fig|243232.1.peg.1699
- TIGR:   MJ1652
- HOGENOM:   HBG512935
- OMA:   QVIDLVW
- ProtClustDB:   PRK07219
- BioCyc:   MJAN243232:MJ_1652-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01057

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 87834; Mature: 87702

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIMTALIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGKKIIVASAVGHLFTLV
CHHHHEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEHHHHHHHHHH
EKENKEFGFYPVFDIKWVPASVDKGKEYVNKYIKALKKLSKDADEFYIATDWDIEGELIG
HHCCCCCCCEEEEEEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
YHALKYCCGREKAKRMRFSSLTKKEIVRAFENPDEIDYGLVDAGESRHILDWYFGINLSR
HHHHHHHHCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
ALMNAIRAVNRWKTMSVGRVQGPALAFLTERELEIKKFIPKPYWVIEALLKDNLKAIHEK
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EKFWNEKEAKNVYEKIKDEKSAKVVEIKKTKRKLKPLPPFDLGTLQREAYSYFKISPKET
HHHCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHEECCHHHH
QEIAQKLYENALISYPRTSSQKLPKDRKYLEDILNIIKNHPVYGKWAERILKENLKPVEG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
KKEDPAHPAIHIVDIPKEELSEKEKEIYDLIARRTLAAFWDNAEREYLNVKIDIKGEKFK
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEE
LSGSRTVKEGWHEIYYFPKFDEIELPPLKKNDIIKVEKITITRKETQPPKRYTVASIIKE
ECCCCCHHHCHHHEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHH
LEKRGLGTKATRAEIIDKLIKRGYVIDDGSLKVTDLGISVIETLKRFCPEIIDEKMTRDL
HHHCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EEKLEKIQFRKIKKDDVLDEAEKRLRKILEEFKKKEEDIGIYLIKNLDATNKKAKIVGKC
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECC
PKCGGDLILIRHKKGRFVGCSNYPECDVKYSLPDKGRIKIPNKVCDACKSPILKIGDREI
CCCCCCEEEEEECCCCEECCCCCCCCCEEECCCCCCCEECCHHHHHHHCCCCEECCCCEE
CINPECPLKQVEVKEEDRICPKCGAKLILKKGVYGAFYGCSNYPKCKYTEPINKKEVVGK
EECCCCCCCCCCCCHHCCCCHHHCHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHCC
CPKCGGDLVVREGKFGKFVGCSNYPKCRYTEKLKLNEKEEK
CCCCCCCEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEIMTALIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGKKIIVASAVGHLFTLV
HHHHEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEHHHHHHHHHH
EKENKEFGFYPVFDIKWVPASVDKGKEYVNKYIKALKKLSKDADEFYIATDWDIEGELIG
HHCCCCCCCEEEEEEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
YHALKYCCGREKAKRMRFSSLTKKEIVRAFENPDEIDYGLVDAGESRHILDWYFGINLSR
HHHHHHHHCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
ALMNAIRAVNRWKTMSVGRVQGPALAFLTERELEIKKFIPKPYWVIEALLKDNLKAIHEK
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EKFWNEKEAKNVYEKIKDEKSAKVVEIKKTKRKLKPLPPFDLGTLQREAYSYFKISPKET
HHHCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHEECCHHHH
QEIAQKLYENALISYPRTSSQKLPKDRKYLEDILNIIKNHPVYGKWAERILKENLKPVEG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
KKEDPAHPAIHIVDIPKEELSEKEKEIYDLIARRTLAAFWDNAEREYLNVKIDIKGEKFK
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEE
LSGSRTVKEGWHEIYYFPKFDEIELPPLKKNDIIKVEKITITRKETQPPKRYTVASIIKE
ECCCCCHHHCHHHEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHH
LEKRGLGTKATRAEIIDKLIKRGYVIDDGSLKVTDLGISVIETLKRFCPEIIDEKMTRDL
HHHCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EEKLEKIQFRKIKKDDVLDEAEKRLRKILEEFKKKEEDIGIYLIKNLDATNKKAKIVGKC
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECC
PKCGGDLILIRHKKGRFVGCSNYPECDVKYSLPDKGRIKIPNKVCDACKSPILKIGDREI
CCCCCCEEEEEECCCCEECCCCCCCCCEEECCCCCCCEECCHHHHHHHCCCCEECCCCEE
CINPECPLKQVEVKEEDRICPKCGAKLILKKGVYGAFYGCSNYPKCKYTEPINKKEVVGK
EECCCCCCCCCCCCHHCCCCHHHCHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHCC
CPKCGGDLVVREGKFGKFVGCSNYPKCRYTEKLKLNEKEEK
CCCCCCCEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087