Click here to switch to the map view.

The map label for this gene is ogt

Identifier: 15669724

GI number: 15669724

Start: 1506419

End: 1506922

Strand: Direct

Name: ogt

Synonym: MJ1529

Alternate gene names: 15669724

Gene position: 1506419-1506922 (Clockwise)

Preceding gene: 15669720

Following gene: 15669725

Centisome position: 90.48

GC content: 28.17

Gene sequence:

>504_bases
ATGATTATTCAGATAGAAGAGTATTTTATAGGGATGATATTTAAAGGTAATCAATTGGTTAGAAATACAATCCCTTTAAG
AAGGGAAGAGATATTTAATTTCATGGATGGGGAGGTTGTTAGCAATCCAGAAGATGAACATTTAAAAGTAGCTGAAATTA
TATTAAAATTATATTTTGCAGAAATTGATGATAAAAAAGTGAGGGAATTAATAAGTTATAAATTGGAAGTTCCAGAATTT
ACAAAAAAGGTTTTAGATATTGTTAAAGACATAGAATTTGGGAAAACCTTAACTTATGGAGATATTGCTAAAAAACTAAA
CACTTCACCAAGAGCTGTTGGAATGGCTTTAAAAAGAAATCCCTTACCTTTAATAATTCCATGTCACAGAGTTGTTGCTA
AAAATTCATTGGGAGGGTATTCCTATGGATTAGACAAGAAAAAATTTATCTTAGAGAGAGAAAGATTAAATATGGTGTCT
TTCAAATTTAATAAAGTCTATTAA

Upstream 100 bases:

>100_bases
TTTCTCAGCAATTTTAGCTATCTCTAAACCTCTATTTCCTATACTTTCATTGTATGTTTTATAGTTAATTACTATCAACA
TAGGCTTTCACCGTGAGAGC

Downstream 100 bases:

>100_bases
TGAATTTTGAACGCTTTCATAAAGGGGGTGTTTATTGGTCCTTAGTATTTCAAAATGTTTTAAAAGACTCTATAAAAAGA
ATTTAAAATAATACTAAAAT

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MIIQIEEYFIGMIFKGNQLVRNTIPLRREEIFNFMDGEVVSNPEDEHLKVAEIILKLYFAEIDDKKVRELISYKLEVPEF
TKKVLDIVKDIEFGKTLTYGDIAKKLNTSPRAVGMALKRNPLPLIIPCHRVVAKNSLGGYSYGLDKKKFILERERLNMVS
FKFNKVY

Sequences:

>Translated_167_residues
MIIQIEEYFIGMIFKGNQLVRNTIPLRREEIFNFMDGEVVSNPEDEHLKVAEIILKLYFAEIDDKKVRELISYKLEVPEF
TKKVLDIVKDIEFGKTLTYGDIAKKLNTSPRAVGMALKRNPLPLIIPCHRVVAKNSLGGYSYGLDKKKFILERERLNMVS
FKFNKVY
>Mature_167_residues
MIIQIEEYFIGMIFKGNQLVRNTIPLRREEIFNFMDGEVVSNPEDEHLKVAEIILKLYFAEIDDKKVRELISYKLEVPEF
TKKVLDIVKDIEFGKTLTYGDIAKKLNTSPRAVGMALKRNPLPLIIPCHRVVAKNSLGGYSYGLDKKKFILERERLNMVS
FKFNKVY

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Saccharomyces cerevisiae, GI6320001, Length=86, Percent_Identity=39.5348837209302, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_METJA (Q58924)

Other databases:

- EMBL:   L77117
- PIR:   H64490
- RefSeq:   NP_248537.1
- PDB:   2G7H
- PDBsum:   2G7H
- ProteinModelPortal:   Q58924
- SMR:   Q58924
- GeneID:   1452437
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1529
- NMPDR:   fig|243232.1.peg.1574
- TIGR:   MJ1529
- HOGENOM:   HBG752398
- OMA:   ILKLYFA
- ProtClustDB:   CLSK2517742
- BioCyc:   MJAN243232:MJ_1529-MONOMER
- BRENDA:   2.1.1.63
- GO:   GO:0005737
- HAMAP:   MF_00772
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 19431; Mature: 19431

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIQIEEYFIGMIFKGNQLVRNTIPLRREEIFNFMDGEVVSNPEDEHLKVAEIILKLYFA
CEEEEEHHEEEEEEECCCHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
EIDDKKVRELISYKLEVPEFTKKVLDIVKDIEFGKTLTYGDIAKKLNTSPRAVGMALKRN
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHCCC
PLPLIIPCHRVVAKNSLGGYSYGLDKKKFILERERLNMVSFKFNKVY
CCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MIIQIEEYFIGMIFKGNQLVRNTIPLRREEIFNFMDGEVVSNPEDEHLKVAEIILKLYFA
CEEEEEHHEEEEEEECCCHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
EIDDKKVRELISYKLEVPEFTKKVLDIVKDIEFGKTLTYGDIAKKLNTSPRAVGMALKRN
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHCCC
PLPLIIPCHRVVAKNSLGGYSYGLDKKKFILERERLNMVSFKFNKVY
CCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087