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The map label for this gene is wecB

Identifier: 15669698

GI number: 15669698

Start: 1475141

End: 1476241

Strand: Reverse

Name: wecB

Synonym: MJ1504

Alternate gene names: 15669698

Gene position: 1476241-1475141 (Counterclockwise)

Preceding gene: 15669699

Following gene: 15669697

Centisome position: 88.66

GC content: 30.61

Gene sequence:

>1101_bases
ATGAAACTCTCTATTATCTTAGGGACAAGACCTGAAATTATAAAACTTTCTCCTATAATTAGAGCTTTAGAAAAAACTAA
CATAGACTGGCATATCATCCACACTAATCAGCATTATTCTGAGAATATGGATAAAATATTCTTTGAGGAGTTAAATCTAC
CAAATCCAAAGTATAATCTTAATATTGGCTCTGGAACTCATGGAGAGCAGACAGGAAAGATGTTAATAGAGATAGAAAAA
GTTCTTTTAAAAGAAAAACCGGATGTTGTTGTAGTTCAGGGAGATACAAACACTGTTTTAGCAGGAGCTTTAGTAGCCTC
AAAATTAAAGATAGATGTAGCTCATGTTGAAGCAGGATTAAGAAGTTTTGATAGAAACATGCCAGAGGAGATAAATAGAG
TTTTGACTGACCATATAAGCAGTTATCTCTTTGCTCCAACTGAAATAGCTAAGAATAATTTATTAAGAGAGGGCATTGAA
GAAAATAAGATTTTTGTTGTGGGAAATACAATTGTTGATGCCACCCTACAAAATTTAAAAATTGCTGAAAAAAATGAAAA
CGTTAGAGCTTTTTTTAATAGTGTTGTTATTGATGATGATTATTTTTTATTAACCCTACATAGGGCTGAAAATGTTGATA
ATAAAGAAAGATTAAAAAATATTGTAGAGGGAATATTTGAGATAATTGAGATATACGATAAAGCTATTATTTTCTCAATC
CATCCACGAACTAAAAAAAGATTGAAAGAGTTTAATTTGTTTGATAAACTAAAAAGCAATAAAAAAATAAAAATTATTGA
GCCAGTTGGCTATTTGGAATTTCTAATGCTGGAAAAAAATGCCGAGCTAATTTTAACAGATAGTGGAGGAGTTCAAGAAG
AGGCATGTATCTTAAAAGTCCCATGTATAACTTTGAGAGACAATACTGAGAGGCCAGAAACAGTTGAAGTTGGAGCTAAT
ATATTAGTTGGTGATAACAAAGAAAAGCTAATTAAAGCGGTTGAAATAATGCTCAATAAAAAGAGAAATTGGAAAAATCC
ATTTGGAAATGGGAAAAGTGGAGAAAGAATTGTGAGAATTCTTACTTATGGAAAGTATTAA

Upstream 100 bases:

>100_bases
TTACAGCAAAAGAAGAGGAATTAATGAAAGTTGAAGGAGTCGGAAAAGAGAGAGCTAAAAAGATTAGAGAGGTTTTAACA
GCAGAATATGAGGGATAAAA

Downstream 100 bases:

>100_bases
AATAGGACTTTCGCAGGAATAAATTTTTATTGAACAATGATACCTAAAGGCATCTATTTTCCAAATTTAATAATATAGAC
TGCGAAAGTCCTATTAAAAG

Product: lipopolysaccharide biosynthesis protein WbpI

Products: NA

Alternate protein names: UDP-GlcNAc-2-epimerase

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKLSIILGTRPEIIKLSPIIRALEKTNIDWHIIHTNQHYSENMDKIFFEELNLPNPKYNLNIGSGTHGEQTGKMLIEIEK
VLLKEKPDVVVVQGDTNTVLAGALVASKLKIDVAHVEAGLRSFDRNMPEEINRVLTDHISSYLFAPTEIAKNNLLREGIE
ENKIFVVGNTIVDATLQNLKIAEKNENVRAFFNSVVIDDDYFLLTLHRAENVDNKERLKNIVEGIFEIIEIYDKAIIFSI
HPRTKKRLKEFNLFDKLKSNKKIKIIEPVGYLEFLMLEKNAELILTDSGGVQEEACILKVPCITLRDNTERPETVEVGAN
ILVGDNKEKLIKAVEIMLNKKRNWKNPFGNGKSGERIVRILTYGKY

Sequences:

>Translated_366_residues
MKLSIILGTRPEIIKLSPIIRALEKTNIDWHIIHTNQHYSENMDKIFFEELNLPNPKYNLNIGSGTHGEQTGKMLIEIEK
VLLKEKPDVVVVQGDTNTVLAGALVASKLKIDVAHVEAGLRSFDRNMPEEINRVLTDHISSYLFAPTEIAKNNLLREGIE
ENKIFVVGNTIVDATLQNLKIAEKNENVRAFFNSVVIDDDYFLLTLHRAENVDNKERLKNIVEGIFEIIEIYDKAIIFSI
HPRTKKRLKEFNLFDKLKSNKKIKIIEPVGYLEFLMLEKNAELILTDSGGVQEEACILKVPCITLRDNTERPETVEVGAN
ILVGDNKEKLIKAVEIMLNKKRNWKNPFGNGKSGERIVRILTYGKY
>Mature_366_residues
MKLSIILGTRPEIIKLSPIIRALEKTNIDWHIIHTNQHYSENMDKIFFEELNLPNPKYNLNIGSGTHGEQTGKMLIEIEK
VLLKEKPDVVVVQGDTNTVLAGALVASKLKIDVAHVEAGLRSFDRNMPEEINRVLTDHISSYLFAPTEIAKNNLLREGIE
ENKIFVVGNTIVDATLQNLKIAEKNENVRAFFNSVVIDDDYFLLTLHRAENVDNKERLKNIVEGIFEIIEIYDKAIIFSI
HPRTKKRLKEFNLFDKLKSNKKIKIIEPVGYLEFLMLEKNAELILTDSGGVQEEACILKVPCITLRDNTERPETVEVGAN
ILVGDNKEKLIKAVEIMLNKKRNWKNPFGNGKSGERIVRILTYGKY

Specific function: Catalyzes the reversible epimerization at C-2 of UDP-N- acetylglucosamine (UDP-GlcNAc) to produce UDP-N-acetylmannosamine (UDP-ManNAc), the activated donor of ManNAc residues

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family

Homologues:

Organism=Escherichia coli, GI48994967, Length=378, Percent_Identity=36.2433862433862, Blast_Score=201, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECB_METJA (Q58899)

Other databases:

- EMBL:   L77117
- PIR:   G64487
- RefSeq:   NP_248511.1
- ProteinModelPortal:   Q58899
- SMR:   Q58899
- GeneID:   1452411
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1504
- NMPDR:   fig|243232.1.peg.1548
- TIGR:   MJ1504
- HOGENOM:   HBG405561
- OMA:   ENMDKIF
- ProtClustDB:   CLSK876603
- BioCyc:   MJAN243232:MJ_1504-MONOMER
- BRENDA:   5.1.3.14
- GO:   GO:0005737
- InterPro:   IPR003331
- PANTHER:   PTHR18964:SF2
- TIGRFAMs:   TIGR00236

Pfam domain/function: PF02350 Epimerase_2

EC number: =5.1.3.14

Molecular weight: Translated: 41727; Mature: 41727

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: ACT_SITE 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSIILGTRPEIIKLSPIIRALEKTNIDWHIIHTNQHYSENMDKIFFEELNLPNPKYNL
CEEEEEECCCCCEEEHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHCCCCCCCEEE
NIGSGTHGEQTGKMLIEIEKVLLKEKPDVVVVQGDTNTVLAGALVASKLKIDVAHVEAGL
ECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHEEEHHHHHHHH
RSFDRNMPEEINRVLTDHISSYLFAPTEIAKNNLLREGIEENKIFVVGNTIVDATLQNLK
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCE
IAEKNENVRAFFNSVVIDDDYFLLTLHRAENVDNKERLKNIVEGIFEIIEIYDKAIIFSI
EECCCCCHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
HPRTKKRLKEFNLFDKLKSNKKIKIIEPVGYLEFLMLEKNAELILTDSGGVQEEACILKV
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEE
PCITLRDNTERPETVEVGANILVGDNKEKLIKAVEIMLNKKRNWKNPFGNGKSGERIVRI
CEEEECCCCCCCCEEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEE
LTYGKY
EECCCC
>Mature Secondary Structure
MKLSIILGTRPEIIKLSPIIRALEKTNIDWHIIHTNQHYSENMDKIFFEELNLPNPKYNL
CEEEEEECCCCCEEEHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHCCCCCCCEEE
NIGSGTHGEQTGKMLIEIEKVLLKEKPDVVVVQGDTNTVLAGALVASKLKIDVAHVEAGL
ECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHEEEHHHHHHHH
RSFDRNMPEEINRVLTDHISSYLFAPTEIAKNNLLREGIEENKIFVVGNTIVDATLQNLK
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCE
IAEKNENVRAFFNSVVIDDDYFLLTLHRAENVDNKERLKNIVEGIFEIIEIYDKAIIFSI
EECCCCCHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
HPRTKKRLKEFNLFDKLKSNKKIKIIEPVGYLEFLMLEKNAELILTDSGGVQEEACILKV
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEE
PCITLRDNTERPETVEVGANILVGDNKEKLIKAVEIMLNKKRNWKNPFGNGKSGERIVRI
CEEEECCCCCCCCEEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEE
LTYGKY
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087