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The map label for this gene is rlmE

Identifier: 15669566

GI number: 15669566

Start: 1324279

End: 1325016

Strand: Direct

Name: rlmE

Synonym: MJ1376

Alternate gene names: 15669566

Gene position: 1324279-1325016 (Clockwise)

Preceding gene: 15669564

Following gene: 15669568

Centisome position: 79.54

GC content: 31.17

Gene sequence:

>738_bases
ATGGGAAGAAAAGATAAAAGATGGGTTTTACAAAGAAAGAGAGATTTCTACTACAAATTAGCTAAAAAACTTAAATATCG
TTCAAGAGCATCTTTTAAACTCATGCAGTTAAATGAAAAATTTAACGTTATTAAACCAGGAAAGATAGTTTTGGATTTGG
GTTGTGCTCCAGGCGGATGGATGCAAGTGGCAAGAGAGATTGTAGGAGATAAAGGCTTTGTTATTGGTATTGACTTACAA
CCAGTTAAGCCATTTGAATATGATAATGTAGTTGCAATAAAAGGAGATTTCACCTTAGAAGAAAATTTGAACAAAATTAG
AGAGCTAATTCCAAATGATGAAAAAAAGGTGGATGTGGTTATAAGTGACGCCTCCCCTAATATAAGCGGTTATTGGGATA
TAGACCACGCTCGTTCAATAGATTTAGTAACTACTGCCTTACAAATAGCTACTGAGATGCTAAAAGAGAGAGGCAATTTT
GTAGCTAAGGTATTTTATGGAGACATGATAGATGATTATGTAAATTTAGTTAAAAAATACTTTGAAAAGGTTTATATTAC
AAAACCTCAAGCTTCAAGAAAAGAAAGTGCTGAGGTTTATGTTATAGCTAAGAGATACACTGGAAAAAAATGGGAAGAAG
AGGATAAGATAAAAAGGGTTAAAAAAGTTGAAAATGAAGATAATGAACTTTTAGCTAAAAAAATTAAAGAGATTAGGAAA
TTAAAATCTAAAAAATAA

Upstream 100 bases:

>100_bases
TTACACAAACTCATAAGAATCAGCTTTTCCTAAGTTTATTTTATTACCTATTATTTCTTTAAAAATTACATTTAATATTT
TTAAAATTTGTGGTGAAACT

Downstream 100 bases:

>100_bases
AAAAATTATTTTTATCTATGTGCATAGCAACATACGAATCCATCATTATCTTTTTTATCTATTCTAACAAATCCAAACCT
CTCAAATTGGATAATATCAT

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MGRKDKRWVLQRKRDFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGWMQVAREIVGDKGFVIGIDLQ
PVKPFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVVISDASPNISGYWDIDHARSIDLVTTALQIATEMLKERGNF
VAKVFYGDMIDDYVNLVKKYFEKVYITKPQASRKESAEVYVIAKRYTGKKWEEEDKIKRVKKVENEDNELLAKKIKEIRK
LKSKK

Sequences:

>Translated_245_residues
MGRKDKRWVLQRKRDFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGWMQVAREIVGDKGFVIGIDLQ
PVKPFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVVISDASPNISGYWDIDHARSIDLVTTALQIATEMLKERGNF
VAKVFYGDMIDDYVNLVKKYFEKVYITKPQASRKESAEVYVIAKRYTGKKWEEEDKIKRVKKVENEDNELLAKKIKEIRK
LKSKK
>Mature_244_residues
GRKDKRWVLQRKRDFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGWMQVAREIVGDKGFVIGIDLQP
VKPFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVVISDASPNISGYWDIDHARSIDLVTTALQIATEMLKERGNFV
AKVFYGDMIDDYVNLVKKYFEKVYITKPQASRKESAEVYVIAKRYTGKKWEEEDKIKRVKKVENEDNELLAKKIKEIRKL
KSKK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI194097365, Length=206, Percent_Identity=38.8349514563107, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI7019377, Length=211, Percent_Identity=37.4407582938389, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI29029591, Length=209, Percent_Identity=35.8851674641148, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI29029589, Length=209, Percent_Identity=35.8851674641148, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI7110661, Length=209, Percent_Identity=35.8851674641148, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=39.7058823529412, Blast_Score=140, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71987561, Length=206, Percent_Identity=39.3203883495146, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17553860, Length=206, Percent_Identity=39.3203883495146, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71987550, Length=206, Percent_Identity=39.3203883495146, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17554650, Length=198, Percent_Identity=36.3636363636364, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17553474, Length=205, Percent_Identity=32.6829268292683, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=197, Percent_Identity=38.0710659898477, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319535, Length=204, Percent_Identity=35.2941176470588, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321302, Length=275, Percent_Identity=28.7272727272727, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18859957, Length=208, Percent_Identity=40.3846153846154, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24648639, Length=215, Percent_Identity=33.4883720930233, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647580, Length=231, Percent_Identity=30.3030303030303, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356387, Length=214, Percent_Identity=29.4392523364486, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_METJA (Q58771)

Other databases:

- EMBL:   L77117
- PIR:   G64471
- RefSeq:   NP_248379.1
- ProteinModelPortal:   Q58771
- SMR:   Q58771
- GeneID:   1452279
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1376
- NMPDR:   fig|243232.1.peg.1416
- TIGR:   MJ1376
- HOGENOM:   HBG398270
- OMA:   DASPNIS
- ProtClustDB:   CLSK876556
- BioCyc:   MJAN243232:MJ_1376-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR004512
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461
- TIGRFAMs:   TIGR00438

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 28560; Mature: 28428

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: ACT_SITE 163-163 BINDING 58-58 BINDING 60-60 BINDING 78-78 BINDING 96-96 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKDKRWVLQRKRDFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCEEEEEECCCCCHH
MQVAREIVGDKGFVIGIDLQPVKPFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVV
HHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEE
ISDASPNISGYWDIDHARSIDLVTTALQIATEMLKERGNFVAKVFYGDMIDDYVNLVKKY
EECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
FEKVYITKPQASRKESAEVYVIAKRYTGKKWEEEDKIKRVKKVENEDNELLAKKIKEIRK
HHHEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKSKK
HHCCC
>Mature Secondary Structure 
GRKDKRWVLQRKRDFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGW
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCEEEEEECCCCCHH
MQVAREIVGDKGFVIGIDLQPVKPFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVV
HHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEE
ISDASPNISGYWDIDHARSIDLVTTALQIATEMLKERGNFVAKVFYGDMIDDYVNLVKKY
EECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
FEKVYITKPQASRKESAEVYVIAKRYTGKKWEEEDKIKRVKKVENEDNELLAKKIKEIRK
HHHEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKSKK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087