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The map label for this gene is carB1

Identifier: 15669568

GI number: 15669568

Start: 1326874

End: 1328322

Strand: Direct

Name: carB1

Synonym: MJ1378

Alternate gene names: 15669568

Gene position: 1326874-1328322 (Clockwise)

Preceding gene: 15669566

Following gene: 15669569

Centisome position: 79.69

GC content: 35.4

Gene sequence:

>1449_bases
ATGGAGAGTATTAAAAAAGTAATGGTTTTTGGTTCTGGACCGATAGTTATTGGACAAGCTGCAGAGTTCGATTTTTCAGG
TTCTCAAGCATGTAAAGCTTTGAAGGAAGAGGGAATTTATACTATTTTAGTGAATTCAAATCCTGCAACTATTCAAACAG
ATACAGACATGGCAGATAAGGTTTATTTAGAGCCATTACATCCAACAATCGTTGAGAAGATTATTGAGAAAGAGAGGCCA
GATGCTATTTTACCAACAATGGGAGGACAAACAGGTCTTAACTTAGCTTTAGAATTACATAGAAGAGGGATTTTAGATAA
ATATGGAATTAAGCTTCTCGGCTCTAATATTAGAACTATTGAAATAGCTGAGGATAGAGAACTTTTTGCCGAGGCAATGG
CTGAAATTAATGAGCCAGTTACAAAGTGTAAGGCCGTTAATTCTGTTGATGAAGCAGTTGAGTTTGCTGAGGAAATTGGC
TATCCAGTCATTGTAAGACCTGCATTTACCTTAGGAGGAACTGGAGGAGGAATTGCCCATAACAAAGAGGAGTTAATAGA
TATTACCTCAAAAGGATTAAAATATTCTATAATCAACCAAGTTTTGATTGATGAGAGTGTTTTAGGATGGAAGGAATTTG
AGCTTGAGGTTATGAGAGATAGAAAAGACACTTGCATCATTGTCTGTGGTATGGAGAACATAGACCCAATGGGAATACAC
ACTGGAGAGAGTATTGTTGTCTCACCTATCCAAACTCTACCAGATGAGTTTTATCAAAAGCTAAGAAACGCTGCTATAAA
GATTATAAGACATTTGGGAATTGAGGGAGGTTGTAATATACAATTTGCTGTAAATAAGGAGATGACTGAATATAGAGTTA
TTGAAGTGAATCCAAGGGTCTCAAGAAGCTCTGCCCTGGCAAGTAAAGCTACAGGTTATCCAATAGCAAGGATTGCCGCT
AAAATAGCCATCGGTAAAACATTAGATGAGATATTAAATGATGTTACAAAGGAAACACCAGCAAGCTTTGAGCCAACTTT
AGATTATGTTGTTGTAAAAATCCCAAGATGGCCATTTGATAAGTTTAAAACAGTAGATAAAAAATTAGGAACAAGTATGA
AGTCCACTGGAGAGGTTATGGCTATAGGTAGAAGCTTTGAAGAAGCTTTGCAAAAGGCAATTAGAAGTTTAGACATTGGA
AGATTCGGCATAATTGGAGATGGAAAGGATAAAGACTATACGGATGAAGAGATAGAAGAAATATTAAAGAATCCAACTGA
TGAAAGAATTTTTGTTATTGCTAAGGCGTTAGAGAAGGGTTGGAGTGTTGAGAAAATAGTTGAGCTAACGGATATTGATG
AATTTTTCATTAAGAAGATTAAGAATATTGTAGATATGAAGAAGGAATTGGAGAAATTAAAAGAAGAAATTAAGAAATTG
AATGCTTAA

Upstream 100 bases:

>100_bases
TAGTTATGCAATTTTAAAAGCTCATCCTCAATATTAAGATAATCTGAATAATAATAGTTAAATATATAAATATTGTAAAA
TATTATTGTGGTGGATATTG

Downstream 100 bases:

>100_bases
TGGGAAATTATGAAAAAAGAAACCAAAATAAATATATTAATTTTGATGAATCTTATATTTGTAGCATTAACATTATTGGC
TTTAATAACTGGAAGAATAT

Product: carbamoyl-phosphate synthase subunit CarB1

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MESIKKVMVFGSGPIVIGQAAEFDFSGSQACKALKEEGIYTILVNSNPATIQTDTDMADKVYLEPLHPTIVEKIIEKERP
DAILPTMGGQTGLNLALELHRRGILDKYGIKLLGSNIRTIEIAEDRELFAEAMAEINEPVTKCKAVNSVDEAVEFAEEIG
YPVIVRPAFTLGGTGGGIAHNKEELIDITSKGLKYSIINQVLIDESVLGWKEFELEVMRDRKDTCIIVCGMENIDPMGIH
TGESIVVSPIQTLPDEFYQKLRNAAIKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRVSRSSALASKATGYPIARIAA
KIAIGKTLDEILNDVTKETPASFEPTLDYVVVKIPRWPFDKFKTVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIG
RFGIIGDGKDKDYTDEEIEEILKNPTDERIFVIAKALEKGWSVEKIVELTDIDEFFIKKIKNIVDMKKELEKLKEEIKKL
NA

Sequences:

>Translated_482_residues
MESIKKVMVFGSGPIVIGQAAEFDFSGSQACKALKEEGIYTILVNSNPATIQTDTDMADKVYLEPLHPTIVEKIIEKERP
DAILPTMGGQTGLNLALELHRRGILDKYGIKLLGSNIRTIEIAEDRELFAEAMAEINEPVTKCKAVNSVDEAVEFAEEIG
YPVIVRPAFTLGGTGGGIAHNKEELIDITSKGLKYSIINQVLIDESVLGWKEFELEVMRDRKDTCIIVCGMENIDPMGIH
TGESIVVSPIQTLPDEFYQKLRNAAIKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRVSRSSALASKATGYPIARIAA
KIAIGKTLDEILNDVTKETPASFEPTLDYVVVKIPRWPFDKFKTVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIG
RFGIIGDGKDKDYTDEEIEEILKNPTDERIFVIAKALEKGWSVEKIVELTDIDEFFIKKIKNIVDMKKELEKLKEEIKKL
NA
>Mature_482_residues
MESIKKVMVFGSGPIVIGQAAEFDFSGSQACKALKEEGIYTILVNSNPATIQTDTDMADKVYLEPLHPTIVEKIIEKERP
DAILPTMGGQTGLNLALELHRRGILDKYGIKLLGSNIRTIEIAEDRELFAEAMAEINEPVTKCKAVNSVDEAVEFAEEIG
YPVIVRPAFTLGGTGGGIAHNKEELIDITSKGLKYSIINQVLIDESVLGWKEFELEVMRDRKDTCIIVCGMENIDPMGIH
TGESIVVSPIQTLPDEFYQKLRNAAIKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRVSRSSALASKATGYPIARIAA
KIAIGKTLDEILNDVTKETPASFEPTLDYVVVKIPRWPFDKFKTVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIG
RFGIIGDGKDKDYTDEEIEEILKNPTDERIFVIAKALEKGWSVEKIVELTDIDEFFIKKIKNIVDMKKELEKLKEEIKKL
NA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=468, Percent_Identity=54.0598290598291, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI21361331, Length=478, Percent_Identity=48.9539748953975, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI169790915, Length=478, Percent_Identity=48.9539748953975, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI170295797, Length=448, Percent_Identity=47.9910714285714, Blast_Score=443, Evalue=1e-124,
Organism=Escherichia coli, GI1786216, Length=484, Percent_Identity=53.5123966942149, Blast_Score=529, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI193204318, Length=473, Percent_Identity=52.8541226215645, Blast_Score=495, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6322569, Length=475, Percent_Identity=53.2631578947368, Blast_Score=514, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6322331, Length=479, Percent_Identity=53.6534446764092, Blast_Score=499, Evalue=1e-142,
Organism=Drosophila melanogaster, GI45555749, Length=468, Percent_Identity=52.3504273504274, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24642586, Length=468, Percent_Identity=52.3504273504274, Blast_Score=496, Evalue=1e-140,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARB1_METJA (Q58773)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_248381.1
- ProteinModelPortal:   Q58773
- SMR:   Q58773
- GeneID:   1452281
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1378
- NMPDR:   fig|243232.1.peg.1418
- TIGR:   MJ1378
- HOGENOM:   HBG405439
- OMA:   EVMAFGR
- ProtClustDB:   CLSK796843
- BioCyc:   MJAN243232:MJ_1378-N-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01210_A
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:1.10.1030.10
- Gene3D:   G3DSA:3.40.50.20
- PRINTS:   PR00098

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; SSF48108 CarbamoylP_synth_lsu_oligo; SSF52440 PreATP-grasp-like

EC number: =6.3.5.5

Molecular weight: Translated: 53610; Mature: 53610

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIKKVMVFGSGPIVIGQAAEFDFSGSQACKALKEEGIYTILVNSNPATIQTDTDMADK
CCCHHEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCH
VYLEPLHPTIVEKIIEKERPDAILPTMGGQTGLNLALELHRRGILDKYGIKLLGSNIRTI
HEECCCCHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCCHHHHCHHEECCCCEEE
EIAEDRELFAEAMAEINEPVTKCKAVNSVDEAVEFAEEIGYPVIVRPAFTLGGTGGGIAH
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCCCC
NKEELIDITSKGLKYSIINQVLIDESVLGWKEFELEVMRDRKDTCIIVCGMENIDPMGIH
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
TGESIVVSPIQTLPDEFYQKLRNAAIKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRV
CCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCC
SRSSALASKATGYPIARIAAKIAIGKTLDEILNDVTKETPASFEPTLDYVVVKIPRWPFD
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCH
KFKTVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIGRFGIIGDGKDKDYTDEEIEE
HHHHHHHHHCCCHHCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
ILKNPTDERIFVIAKALEKGWSVEKIVELTDIDEFFIKKIKNIVDMKKELEKLKEEIKKL
HHHCCCCCHHHHEEHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NA
CC
>Mature Secondary Structure
MESIKKVMVFGSGPIVIGQAAEFDFSGSQACKALKEEGIYTILVNSNPATIQTDTDMADK
CCCHHEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCH
VYLEPLHPTIVEKIIEKERPDAILPTMGGQTGLNLALELHRRGILDKYGIKLLGSNIRTI
HEECCCCHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCCHHHHCHHEECCCCEEE
EIAEDRELFAEAMAEINEPVTKCKAVNSVDEAVEFAEEIGYPVIVRPAFTLGGTGGGIAH
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCCCC
NKEELIDITSKGLKYSIINQVLIDESVLGWKEFELEVMRDRKDTCIIVCGMENIDPMGIH
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
TGESIVVSPIQTLPDEFYQKLRNAAIKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRV
CCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCC
SRSSALASKATGYPIARIAAKIAIGKTLDEILNDVTKETPASFEPTLDYVVVKIPRWPFD
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCH
KFKTVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIGRFGIIGDGKDKDYTDEEIEE
HHHHHHHHHCCCHHCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
ILKNPTDERIFVIAKALEKGWSVEKIVELTDIDEFFIKKIKNIVDMKKELEKLKEEIKKL
HHHCCCCCHHHHEEHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087