Click here to switch to the map view.

The map label for this gene is cobD

Identifier: 15669504

GI number: 15669504

Start: 1259594

End: 1260517

Strand: Direct

Name: cobD

Synonym: MJ1314

Alternate gene names: 15669504

Gene position: 1259594-1260517 (Clockwise)

Preceding gene: 15669503

Following gene: 15669505

Centisome position: 75.65

GC content: 31.71

Gene sequence:

>924_bases
ATGCTGAATCCAATAATCTTATTTTTGGCTATTATTTTTGATAGAATCATTGGGGAGTTGCCAGAGAGTATTCATCCAAC
GGTTTGGATAGGGAAGTTGATAGCTTTTTTAGAGAACATATTTAAATCTACAAATTGCAAAAATAAATATAGAGATTTTT
TGTTTGGCTCACTAACAACATTTATTACTCTATTAGTTGTGGGAGTTATAGCTTTTTTTGTTGATAAATGCATAATGCTG
TTACCATTTCCTTTAAACTATATTATCTATGGTTTTTTGTTATCAACAACTATTGGCTACAAATCATTATTCGAATTCTG
CAAAAAGCCGATTGAATATATAAAAAATGGTGATTTAGAGGGAGCAAGGAAAGCTGTTCAGCATATAGTTAGCAGAGATG
CCTCAAAGTTGGATAAAGAGCATGTATTATCGGCTGCAGTAGAGAGCTTATCCGAGAACATAACAGACAGTATAATTGGA
GCTTTATTCTATGCTATATTTTTTGGTTTGCCTGGAGCCTTTGTTTATAGGGCGATAAATACATTAGATGCAATGATTGG
TTATAAAAATGAGAAATATCTATGGTATGGGAAGTTAGCAGCAAGGTTGGATGATATTGCCAATTTTATTCCTTCAAGAA
TAGCAGGGATTTTGCTAATAATTACTGCCCCATTTTATAAAGGAGATGTTAAAAAGGCAATATATGGGTTTTTAAAAGAA
GCTAATAAGGTTCCATCACCAAACTCTGGTTATACAATGGCTACATTGGCAAATGCATTAAATATAACTTTGGAGAAGAT
AGGATATTATAAACTTGGTAGTGGGAAAATAGATGTTGAAAAATCTTTAAACGCTTTTAAGGCAGTTGATTATACAGTCG
TTGTGTTTTTAATTATTTATACTTTAATTTGGTGGATAACATGA

Upstream 100 bases:

>100_bases
GAGTTGCAACAAACTCTGGAAAAGCTGCTGTTTATCTACCAAGCTTTTGTAAGAGTGAAGTAATTTTTGGAGATATTGAG
GAATTGTTAAAAGGGAGATA

Downstream 100 bases:

>100_bases
TAAGTAAAGCTTATTACACTACAGAGATTCCAGAGGATAGATTTGAAGCTCTGAGTTGTATTAAAGATAGTCAAAAACCT
CTTAAAATTATATTACTTGG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLNPIILFLAIIFDRIIGELPESIHPTVWIGKLIAFLENIFKSTNCKNKYRDFLFGSLTTFITLLVVGVIAFFVDKCIML
LPFPLNYIIYGFLLSTTIGYKSLFEFCKKPIEYIKNGDLEGARKAVQHIVSRDASKLDKEHVLSAAVESLSENITDSIIG
ALFYAIFFGLPGAFVYRAINTLDAMIGYKNEKYLWYGKLAARLDDIANFIPSRIAGILLIITAPFYKGDVKKAIYGFLKE
ANKVPSPNSGYTMATLANALNITLEKIGYYKLGSGKIDVEKSLNAFKAVDYTVVVFLIIYTLIWWIT

Sequences:

>Translated_307_residues
MLNPIILFLAIIFDRIIGELPESIHPTVWIGKLIAFLENIFKSTNCKNKYRDFLFGSLTTFITLLVVGVIAFFVDKCIML
LPFPLNYIIYGFLLSTTIGYKSLFEFCKKPIEYIKNGDLEGARKAVQHIVSRDASKLDKEHVLSAAVESLSENITDSIIG
ALFYAIFFGLPGAFVYRAINTLDAMIGYKNEKYLWYGKLAARLDDIANFIPSRIAGILLIITAPFYKGDVKKAIYGFLKE
ANKVPSPNSGYTMATLANALNITLEKIGYYKLGSGKIDVEKSLNAFKAVDYTVVVFLIIYTLIWWIT
>Mature_307_residues
MLNPIILFLAIIFDRIIGELPESIHPTVWIGKLIAFLENIFKSTNCKNKYRDFLFGSLTTFITLLVVGVIAFFVDKCIML
LPFPLNYIIYGFLLSTTIGYKSLFEFCKKPIEYIKNGDLEGARKAVQHIVSRDASKLDKEHVLSAAVESLSENITDSIIG
ALFYAIFFGLPGAFVYRAINTLDAMIGYKNEKYLWYGKLAARLDDIANFIPSRIAGILLIITAPFYKGDVKKAIYGFLKE
ANKVPSPNSGYTMATLANALNITLEKIGYYKLGSGKIDVEKSLNAFKAVDYTVVVFLIIYTLIWWIT

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_METJA (Q58710)

Other databases:

- EMBL:   L77117
- PIR:   A64464
- RefSeq:   NP_248314.1
- GeneID:   1452216
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1314
- NMPDR:   fig|243232.1.peg.1351
- TIGR:   MJ1314
- HOGENOM:   HBG647131
- OMA:   KINSDKP
- ProtClustDB:   PRK01209
- BioCyc:   MJAN243232:MJ_1314-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 34502; Mature: 34502

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x8af9904)-; HASH(0xc3aca88)-; HASH(0xe19451c)-; HASH(0xe4c1f5c)-; HASH(0xe3dd6ac)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPIILFLAIIFDRIIGELPESIHPTVWIGKLIAFLENIFKSTNCKNKYRDFLFGSLTT
CCHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FITLLVVGVIAFFVDKCIMLLPFPLNYIIYGFLLSTTIGYKSLFEFCKKPIEYIKNGDLE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
GARKAVQHIVSRDASKLDKEHVLSAAVESLSENITDSIIGALFYAIFFGLPGAFVYRAIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TLDAMIGYKNEKYLWYGKLAARLDDIANFIPSRIAGILLIITAPFYKGDVKKAIYGFLKE
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ANKVPSPNSGYTMATLANALNITLEKIGYYKLGSGKIDVEKSLNAFKAVDYTVVVFLIIY
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLIWWIT
HHHHHCH
>Mature Secondary Structure
MLNPIILFLAIIFDRIIGELPESIHPTVWIGKLIAFLENIFKSTNCKNKYRDFLFGSLTT
CCHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FITLLVVGVIAFFVDKCIMLLPFPLNYIIYGFLLSTTIGYKSLFEFCKKPIEYIKNGDLE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
GARKAVQHIVSRDASKLDKEHVLSAAVESLSENITDSIIGALFYAIFFGLPGAFVYRAIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TLDAMIGYKNEKYLWYGKLAARLDDIANFIPSRIAGILLIITAPFYKGDVKKAIYGFLKE
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ANKVPSPNSGYTMATLANALNITLEKIGYYKLGSGKIDVEKSLNAFKAVDYTVVVFLIIY
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLIWWIT
HHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087