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The map label for this gene is bioF [C]

Identifier: 15669488

GI number: 15669488

Start: 1245351

End: 1246469

Strand: Direct

Name: bioF [C]

Synonym: MJ1298

Alternate gene names: 15669488

Gene position: 1245351-1246469 (Clockwise)

Preceding gene: 15669487

Following gene: 15669489

Centisome position: 74.8

GC content: 32.98

Gene sequence:

>1119_bases
ATGTTTAGGGAGAAATTAAGAAGAGAGATTGAAATTATAAAGAACAACGGATTATATAGATTTTTAAGGAAAAAAGATGA
TGGAGTTTTAGATTTCTCTTCAAATGATTATCTATGCCTATCAAAGCATCCAGAGGTTATTGAAGCAGTTAAAGAAGGGC
TAAAATATGGGGCTGGTTCAACCGGCTCAAGATTAACTTCTGGAAATATAAACCATCAAAGATTGGAGGAGAAGATAGCT
GAATTTAAAGAGACGGAGAGGACTTTAGTTTATTCATCCGGCTATGCAACGAATGTTGGAGTTATCTCTGCATTGTGCAA
AAAAGGAGATTTAATTTTGAGTGATAAGCTTAATCATGCCTCTATCATTGATGGTTGCAAGTTGAGTAAAGCAGATGTTT
TAATTTACAATCATTGCGATGTTGAGCATTTAACCAACTTAATTGAAGAGAATTGGGGGAAATATAACAACCTCTTTATT
GTAACTGATGGAGTTTTTAGTATGGATGGGGATATAGCTCCTTTGAGGGATTTAAAGAAGATAGCTGATGAATTTAATGC
CATTTTAATTATTGATGACGCACACGGCACTGGAGTTTTAGGAGATGGGAGAGGAACATTAAAGCACTTTAATTTAAAAC
CTTCTGACAACATAGTGCAAATTGGAACTTTATCTAAAGCAATTGGTGGTTTAGGAGGCTTTGTCTGTGGAATTGAGGAG
GTTGTAGAGTATTTAATAAACACTTCGAGGAGTTTTATATTCTCAACTGCTCTACCTCCTCATGTAGTTGAGGGTTGCAT
TAAGGCCTTTGAGATTATTGAAAAAACAGATATAGTTAAAAAGCTTCAAAAAAACATAAAAATAGCAAATAAAGTTTTTA
AAAAATATGAATTTATTAAAGAAGATAACTTAACTCCAATCTATCCATTTATTTTTAAAGAAAAAACCATGGAAATAGCA
GAACATTTAATAAAAAATAATATCTTTTGTGTAGGAATTAGATATCCAACTGTTCCTAAGGGATTGGAGAGGATAAGGGT
TAGCATAAATGTTGGGCATGAAAAAGAGGATTTTGAACTGTTGTGTGAGAGGATTAAAGAGGTTTATAGTAGTGACTGA

Upstream 100 bases:

>100_bases
TGGAGGAAGGGTATTTTTTGTTAAAGATGATGTTGATATAGAGGATTTAATCAACAAATTAGAAAACAAACCATTTATTA
TAAAATAAAAAGGGATTTTT

Downstream 100 bases:

>100_bases
TATTTCTCTTTTTTAGTTTTTATTAAATAAAATAAGCATTTATTTGGTTTCCTATATCAATGATAATTGTAATTATAATA
ATTACGATTATCATTTTGTG

Product: 8-amino-7-oxononanoate synthase BioF

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFREKLRREIEIIKNNGLYRFLRKKDDGVLDFSSNDYLCLSKHPEVIEAVKEGLKYGAGSTGSRLTSGNINHQRLEEKIA
EFKETERTLVYSSGYATNVGVISALCKKGDLILSDKLNHASIIDGCKLSKADVLIYNHCDVEHLTNLIEENWGKYNNLFI
VTDGVFSMDGDIAPLRDLKKIADEFNAILIIDDAHGTGVLGDGRGTLKHFNLKPSDNIVQIGTLSKAIGGLGGFVCGIEE
VVEYLINTSRSFIFSTALPPHVVEGCIKAFEIIEKTDIVKKLQKNIKIANKVFKKYEFIKEDNLTPIYPFIFKEKTMEIA
EHLIKNNIFCVGIRYPTVPKGLERIRVSINVGHEKEDFELLCERIKEVYSSD

Sequences:

>Translated_372_residues
MFREKLRREIEIIKNNGLYRFLRKKDDGVLDFSSNDYLCLSKHPEVIEAVKEGLKYGAGSTGSRLTSGNINHQRLEEKIA
EFKETERTLVYSSGYATNVGVISALCKKGDLILSDKLNHASIIDGCKLSKADVLIYNHCDVEHLTNLIEENWGKYNNLFI
VTDGVFSMDGDIAPLRDLKKIADEFNAILIIDDAHGTGVLGDGRGTLKHFNLKPSDNIVQIGTLSKAIGGLGGFVCGIEE
VVEYLINTSRSFIFSTALPPHVVEGCIKAFEIIEKTDIVKKLQKNIKIANKVFKKYEFIKEDNLTPIYPFIFKEKTMEIA
EHLIKNNIFCVGIRYPTVPKGLERIRVSINVGHEKEDFELLCERIKEVYSSD
>Mature_372_residues
MFREKLRREIEIIKNNGLYRFLRKKDDGVLDFSSNDYLCLSKHPEVIEAVKEGLKYGAGSTGSRLTSGNINHQRLEEKIA
EFKETERTLVYSSGYATNVGVISALCKKGDLILSDKLNHASIIDGCKLSKADVLIYNHCDVEHLTNLIEENWGKYNNLFI
VTDGVFSMDGDIAPLRDLKKIADEFNAILIIDDAHGTGVLGDGRGTLKHFNLKPSDNIVQIGTLSKAIGGLGGFVCGIEE
VVEYLINTSRSFIFSTALPPHVVEGCIKAFEIIEKTDIVKKLQKNIKIANKVFKKYEFIKEDNLTPIYPFIFKEKTMEIA
EHLIKNNIFCVGIRYPTVPKGLERIRVSINVGHEKEDFELLCERIKEVYSSD

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=348, Percent_Identity=37.9310344827586, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI284448556, Length=349, Percent_Identity=37.8223495702006, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4758668, Length=363, Percent_Identity=32.5068870523416, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI119220554, Length=376, Percent_Identity=29.5212765957447, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI83977444, Length=351, Percent_Identity=29.9145299145299, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI83977440, Length=351, Percent_Identity=29.9145299145299, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI83977442, Length=351, Percent_Identity=29.9145299145299, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4502025, Length=345, Percent_Identity=31.0144927536232, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI40316939, Length=345, Percent_Identity=31.0144927536232, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5454084, Length=372, Percent_Identity=27.4193548387097, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1786993, Length=356, Percent_Identity=33.4269662921348, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1790046, Length=387, Percent_Identity=33.8501291989664, Blast_Score=207, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI71994529, Length=389, Percent_Identity=37.2750642673522, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI32566772, Length=362, Percent_Identity=30.1104972375691, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560914, Length=316, Percent_Identity=28.4810126582279, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560912, Length=316, Percent_Identity=28.4810126582279, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71982625, Length=272, Percent_Identity=30.1470588235294, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71982617, Length=272, Percent_Identity=30.1470588235294, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320438, Length=378, Percent_Identity=30.952380952381, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320267, Length=364, Percent_Identity=27.4725274725275, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6323954, Length=292, Percent_Identity=32.1917808219178, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24662918, Length=378, Percent_Identity=33.5978835978836, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17137420, Length=380, Percent_Identity=31.5789473684211, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17136286, Length=361, Percent_Identity=26.5927977839335, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653280, Length=374, Percent_Identity=27.807486631016, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24653276, Length=374, Percent_Identity=27.807486631016, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24653278, Length=374, Percent_Identity=27.807486631016, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIKB_METJA (Q58694)

Other databases:

- EMBL:   L77117
- PIR:   A64462
- RefSeq:   NP_248298.1
- ProteinModelPortal:   Q58694
- GeneID:   1452200
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1298
- NMPDR:   fig|243232.1.peg.1335
- TIGR:   MJ1298
- HOGENOM:   HBG649839
- OMA:   NTSRSFI
- ProtClustDB:   CLSK876517
- BioCyc:   MJAN243232:MJ_1298-MONOMER
- BRENDA:   2.3.1.47
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.29; =2.3.1.47

Molecular weight: Translated: 41896; Mature: 41896

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 20-20 BINDING 119-119 BINDING 167-167 BINDING 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFREKLRREIEIIKNNGLYRFLRKKDDGVLDFSSNDYLCLSKHPEVIEAVKEGLKYGAGS
CCHHHHHHHHHHHHCCCCEEEHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHCCCCC
TGSRLTSGNINHQRLEEKIAEFKETERTLVYSSGYATNVGVISALCKKGDLILSDKLNHA
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCH
SIIDGCKLSKADVLIYNHCDVEHLTNLIEENWGKYNNLFIVTDGVFSMDGDIAPLRDLKK
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCHHHHHHH
IADEFNAILIIDDAHGTGVLGDGRGTLKHFNLKPSDNIVQIGTLSKAIGGLGGFVCGIEE
HHHHCCEEEEEECCCCCCEEECCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHH
VVEYLINTSRSFIFSTALPPHVVEGCIKAFEIIEKTDIVKKLQKNIKIANKVFKKYEFIK
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDNLTPIYPFIFKEKTMEIAEHLIKNNIFCVGIRYPTVPKGLERIRVSINVGHEKEDFEL
CCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHEEEEEEECCCCCHHHHH
LCERIKEVYSSD
HHHHHHHHHCCC
>Mature Secondary Structure
MFREKLRREIEIIKNNGLYRFLRKKDDGVLDFSSNDYLCLSKHPEVIEAVKEGLKYGAGS
CCHHHHHHHHHHHHCCCCEEEHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHCCCCC
TGSRLTSGNINHQRLEEKIAEFKETERTLVYSSGYATNVGVISALCKKGDLILSDKLNHA
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCH
SIIDGCKLSKADVLIYNHCDVEHLTNLIEENWGKYNNLFIVTDGVFSMDGDIAPLRDLKK
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCHHHHHHH
IADEFNAILIIDDAHGTGVLGDGRGTLKHFNLKPSDNIVQIGTLSKAIGGLGGFVCGIEE
HHHHCCEEEEEECCCCCCEEECCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHH
VVEYLINTSRSFIFSTALPPHVVEGCIKAFEIIEKTDIVKKLQKNIKIANKVFKKYEFIK
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDNLTPIYPFIFKEKTMEIAEHLIKNNIFCVGIRYPTVPKGLERIRVSINVGHEKEDFEL
CCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHEEEEEEECCCCCHHHHH
LCERIKEVYSSD
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087