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The map label for this gene is bioD [H]

Identifier: 15669489

GI number: 15669489

Start: 1246513

End: 1247259

Strand: Direct

Name: bioD [H]

Synonym: MJ1299

Alternate gene names: 15669489

Gene position: 1246513-1247259 (Clockwise)

Preceding gene: 15669488

Following gene: 15669490

Centisome position: 74.87

GC content: 28.11

Gene sequence:

>747_bases
TTGGTTTCCTATATCAATGATAATTGTAATTATAATAATTACGATTATCATTTTGTGGTAAGTTATTTAAACAGTAGATT
AAAAATTTTGATAGGTGATAAAATGATATTTATAACTGGAACAGACACTGGTATAGGGAAAACTTACGTTTCATCAATTT
TAGCAGAGAATTTGAAAAAAATGGGCATTAACGTTGGATATTTAAAGCCGGTTGAGACAGGAGGGAGAGAAGACACTCTA
ACTTTAAAAAATATTTTAAATACAGATGATGATTTGGATTTAATGAATCCTATTAATTTAAAACTTCCCCTATCTCCAAA
TATTGCGTTTGACGTTGAAAACTCCCCTTTAACTTTGGATGAGATAAAAGAGAAAATAAAAAATGCTTATGAAACTTTAA
AAGAAAAATATGATTTTTTGATTGTTGAAGGAGCTGGAGGAGTTTGCGTTCCAATAAAAGAAGATTTTTTAATGAGTGAT
TTGATTAAGTTTTTAGGATTAGATGCAGTTGTTGTTTCAAGACCTAATTTAGGGACTATAAACCACACACTATTAACTGT
TGAACATTTAAGGAATAAAGGAATTAACGTTAGAGGAGTTATTATTAACTGTATAACTGATTTAAGTGAAGTTTTATATT
ATGAAAAAACCTTTGAAACAATAGAAAAAGTTGGGAACATTGAAATAATTGGGATTGTTAAAAGTAGGGAAGATTTTGAG
ATTGATTTTGAAAAGATTTTGAGGTGA

Upstream 100 bases:

>100_bases
AAAGAGGATTTTGAACTGTTGTGTGAGAGGATTAAAGAGGTTTATAGTAGTGACTGATATTTCTCTTTTTTAGTTTTTAT
TAAATAAAATAAGCATTTAT

Downstream 100 bases:

>100_bases
ATAAAATGAACATTGATAAAAATTTACTTGAAAAATGGGATAAAGAATATATTTGGCATCCATACACACAGATGAAAGAA
TATAGAGAATCAAAAAACTT

Product: dethiobiotin synthetase BioD

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MVSYINDNCNYNNYDYHFVVSYLNSRLKILIGDKMIFITGTDTGIGKTYVSSILAENLKKMGINVGYLKPVETGGREDTL
TLKNILNTDDDLDLMNPINLKLPLSPNIAFDVENSPLTLDEIKEKIKNAYETLKEKYDFLIVEGAGGVCVPIKEDFLMSD
LIKFLGLDAVVVSRPNLGTINHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFE
IDFEKILR

Sequences:

>Translated_248_residues
MVSYINDNCNYNNYDYHFVVSYLNSRLKILIGDKMIFITGTDTGIGKTYVSSILAENLKKMGINVGYLKPVETGGREDTL
TLKNILNTDDDLDLMNPINLKLPLSPNIAFDVENSPLTLDEIKEKIKNAYETLKEKYDFLIVEGAGGVCVPIKEDFLMSD
LIKFLGLDAVVVSRPNLGTINHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFE
IDFEKILR
>Mature_248_residues
MVSYINDNCNYNNYDYHFVVSYLNSRLKILIGDKMIFITGTDTGIGKTYVSSILAENLKKMGINVGYLKPVETGGREDTL
TLKNILNTDDDLDLMNPINLKLPLSPNIAFDVENSPLTLDEIKEKIKNAYETLKEKYDFLIVEGAGGVCVPIKEDFLMSD
LIKFLGLDAVVVSRPNLGTINHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFE
IDFEKILR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=182, Percent_Identity=30.2197802197802, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081944, Length=180, Percent_Identity=31.6666666666667, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324385, Length=197, Percent_Identity=38.0710659898477, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 28007; Mature: 28007

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSYINDNCNYNNYDYHFVVSYLNSRLKILIGDKMIFITGTDTGIGKTYVSSILAENLKK
CCEEECCCCCCCCCCHHHHEEHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHH
MGINVGYLKPVETGGREDTLTLKNILNTDDDLDLMNPINLKLPLSPNIAFDVENSPLTLD
HCCCCEEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEHH
EIKEKIKNAYETLKEKYDFLIVEGAGGVCVPIKEDFLMSDLIKFLGLDAVVVSRPNLGTI
HHHHHHHHHHHHHHHCCCEEEEECCCCEEEECHHHHHHHHHHHHHCCCEEEEECCCCCCC
NHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFE
HHHHEEHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
IDFEKILR
CCHHHHCC
>Mature Secondary Structure
MVSYINDNCNYNNYDYHFVVSYLNSRLKILIGDKMIFITGTDTGIGKTYVSSILAENLKK
CCEEECCCCCCCCCCHHHHEEHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHH
MGINVGYLKPVETGGREDTLTLKNILNTDDDLDLMNPINLKLPLSPNIAFDVENSPLTLD
HCCCCEEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEHH
EIKEKIKNAYETLKEKYDFLIVEGAGGVCVPIKEDFLMSDLIKFLGLDAVVVSRPNLGTI
HHHHHHHHHHHHHHHCCCEEEEECCCCEEEECHHHHHHHHHHHHHCCCEEEEECCCCCCC
NHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFE
HHHHEEHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
IDFEKILR
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]