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The map label for this gene is leuD

Identifier: 15669463

GI number: 15669463

Start: 1222548

End: 1223054

Strand: Reverse

Name: leuD

Synonym: MJ1277

Alternate gene names: 15669463

Gene position: 1223054-1222548 (Counterclockwise)

Preceding gene: 15669464

Following gene: 15669462

Centisome position: 73.46

GC content: 36.29

Gene sequence:

>507_bases
ATGAGAAGTATAATAAAGGGAAGAGTTTGGAAGTTTGGAAATAACGTAGATACAGATGCTATATTACCAGCAAGGTATTT
AGTTTATACAAAACCAGAGGAATTAGCTCAGTTTGTTATGACTGGGGCAGACCCAGATTTTCCAAAGAAGGTTAAGCCAG
GAGATATAATAGTTGGAGGAAAGAACTTTGGATGTGGTTCAAGTAGAGAGCATGCCCCATTAGGATTAAAAGGAGCTGGA
ATCAGCTGTGTTATTGCTGAGAGCTTCGCAAGAATATTTTATAGAAATGCCATAAATGTTGGATTACCATTAATTGAATG
TAAGGGCATTTCAGAGAAAGTCAATGAAGGGGATGAGTTAGAGGTTAATTTAGAGACTGGAGAGATTAAAAACTTAACCA
CTGGAGAGGTTTTAAAAGGTCAAAAATTACCAGAATTCATGATGGAAATTTTAGAGGCTGGAGGATTAATGCCATACTTA
AAGAAAAAGATGGCTGAAAGCCAATAA

Upstream 100 bases:

>100_bases
TAGATTTTCATATTAAACAATACAGAAAACAAAACTTATTTATTCACTTACTTTTTATTTTAGTTATAATCTACATTAAT
CATATTCAAAAGGTGAAATA

Downstream 100 bases:

>100_bases
TTTTATTTTTTGGTGGTAATTATGGCTACACAGACGATAACTTTAACTTTTGAAATTCCAGAATTTATTGATAAAAATAA
ATTCAAAAAGGAGCTTGAAA

Product: 3-isopropylmalate dehydratase LeuD

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MRSIIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGGKNFGCGSSREHAPLGLKGAG
ISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDELEVNLETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYL
KKKMAESQ

Sequences:

>Translated_168_residues
MRSIIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGGKNFGCGSSREHAPLGLKGAG
ISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDELEVNLETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYL
KKKMAESQ
>Mature_168_residues
MRSIIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGGKNFGCGSSREHAPLGLKGAG
ISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDELEVNLETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYL
KKKMAESQ

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family

Homologues:

Organism=Escherichia coli, GI1786258, Length=163, Percent_Identity=31.9018404907975, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=173, Percent_Identity=35.8381502890173, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=42.7184466019417, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_METJA (Q58673)

Other databases:

- EMBL:   L77117
- PIR:   D64459
- RefSeq:   NP_248273.1
- ProteinModelPortal:   Q58673
- SMR:   Q58673
- GeneID:   1452175
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1277
- NMPDR:   fig|243232.1.peg.1310
- TIGR:   MJ1277
- HOGENOM:   HBG304838
- OMA:   PGRYLIF
- ProtClustDB:   CLSK876515
- BioCyc:   MetaCyc:MONOMER-13648
- BioCyc:   MJAN243232:MJ_1277-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31

Molecular weight: Translated: 18378; Mature: 18378

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGG
CCCCCCCCCEECCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEECC
KNFGCGSSREHAPLGLKGAGISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDEL
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCEE
EVNLETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYLKKKMAESQ
EEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRSIIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGG
CCCCCCCCCEECCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEECC
KNFGCGSSREHAPLGLKGAGISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDEL
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCEE
EVNLETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYLKKKMAESQ
EEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087