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The map label for this gene is ilvD [H]

Identifier: 15669462

GI number: 15669462

Start: 1220645

End: 1222330

Strand: Reverse

Name: ilvD [H]

Synonym: MJ1276

Alternate gene names: 15669462

Gene position: 1222330-1220645 (Counterclockwise)

Preceding gene: 15669463

Following gene: 15669460

Centisome position: 73.41

GC content: 38.14

Gene sequence:

>1686_bases
TTGAATTTTATGAAGAGGGAGAAGATGATAAGTGATAGAGTAAAAAAAGGATTAAAAAGAGCTCCAAATAGAAGTTTATT
AAAGGCTTGTGGATATACAGATGAGGAATTGGAGAGACCATTTATTGGAGTTGTTAATAGCTTTACCGAAGTTGTTCCTG
GGCATATTCATTTAAGAGATATTGCTGAGGCAGTTAAAAAAGGAATTTACGCAAATGGAGGAACTGCCTTTGAATTCAAC
ACAATGGCAATATGTGATGGAATAGCAATGGGACATGAGGGGATGAAATATTCCTTACCTTCAAGGGAAATTATAGCAGA
TACTGTAGAGAGTATGGCAAAAGCTCATGGATTTGATGGATTAGTTTTAATTCCTTCATGCGACAAAATAGTTCCTGGAA
TGATAATGGGAGCTATAAGAACTGGATTACCATTTATAGTTGTTACTGGGGGGCCGATGTTTCCTGGAGAGTTGAGAGGG
AAAAAGTATGATTTAATTAGTGTATTTGAGGGAGTTGGAGCTTGTGCAGCTGGAAAAATTACAGAGGAAGAACTTAAAGA
GATTGAAGATATTGCCTGCCCAGGAGCTGGTAGTTGTGCTGGACTATTTACAGCAAATACCATGGCTTGCTTAACAGAGG
CTATGGGCCTCTCTTTGCCATATTGTGCAACATCACATGCAACAACAGCAGAGAAGATAAGAATAGCTAAAAGAAGTGGG
ATGAGAATAGTTGATTTAGTTAGAAACAACATAACTCCAGATAAGATTTTAACTAAGGAGGCATTTGAAAATGCCATTTT
GGTAGATTTAGCTTTGGGTGGTTCAACAAATACAACTCTACATATTCCGGCAATAGCAAATGAGGTAAAGCCAAAGTTCA
TAACATTGGATGACTTTGATAGATTATCTGGTGAAGTTCCTCACATAGCTTCTTTAAGACCTGGTGGAGAGCACTTTATA
ATTGACTTGCACAGAGCTGGAGGAATTCCAGCTGTTTTAAAGGTTTTAGAGGAAAAAATAAGAAAAGAATGCTTAACAGT
TAGTGGAAAAACCATTGGAGAAATAATTAAAGAGGTTAAATACATTGATTATAGTGTAATAAGACCTGTAGATAATCCAG
TTCATGAAACAGCTGGTTTGAGAATATTGAAAGGAAGCTTAGCTCCTAACGGAGCAGTTGTTAAAATCGGAGCTGTAAAT
CCAAAAATGTATAAGCATGAAGGGCCTGCAAGAGTCTTTGATAGTGAGGAAGAGGCAGTTGATGCTATATTGGGGGGAGA
TATTGAGAGAGGAGATGTTGTGGTTATCAGATATGAGGGGCCTGCAGGAGGGCCAGGAATGAGGGAAATGTTGGCTCCAA
CTTCAGCAATATGTGGAATGGGGTTGGATGATTCTGTCGCTTTAATTACAGATGGAAGATTCAGCGGAGGAAGTAGAGGA
CCGTGTATTGGGCACGTTTCTCCAGAGGCAATGGCTGGAGGTCCGATAGCGATAGTTGAAGATGGAGATATTATAAAAAT
AGACATGATAAACAAGAAGTTGGATTTAGCTTTAGATGAAGAAGAGATTAAAGAGAGATTAGCCAAATGGAAAAAACCTG
AACCTAAGGTTAAAAAAGGTTATTTAGCAAGATATGCTAAGCTTGTAAGTTCAGCTGATGAGGGAGCTGTATTAAGATAT
GATTAA

Upstream 100 bases:

>100_bases
ATCTTGGGAGAGAAATTCTATAAACTTATGGAAAAGGTGTAGATGTTGAAAAAATTGAAAAAGAATGTGAAGAATTTAGA
AAAAAATTCAAATTCAGAAA

Downstream 100 bases:

>100_bases
TAGAGATTTCTTTATGCTTATTGTATTTTTTACATAATATTTTTATTACCAATTTATAATTTTGTCGTAATACACTAGGA
CTAGGATTTTTAATTTTATA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MNFMKREKMISDRVKKGLKRAPNRSLLKACGYTDEELERPFIGVVNSFTEVVPGHIHLRDIAEAVKKGIYANGGTAFEFN
TMAICDGIAMGHEGMKYSLPSREIIADTVESMAKAHGFDGLVLIPSCDKIVPGMIMGAIRTGLPFIVVTGGPMFPGELRG
KKYDLISVFEGVGACAAGKITEEELKEIEDIACPGAGSCAGLFTANTMACLTEAMGLSLPYCATSHATTAEKIRIAKRSG
MRIVDLVRNNITPDKILTKEAFENAILVDLALGGSTNTTLHIPAIANEVKPKFITLDDFDRLSGEVPHIASLRPGGEHFI
IDLHRAGGIPAVLKVLEEKIRKECLTVSGKTIGEIIKEVKYIDYSVIRPVDNPVHETAGLRILKGSLAPNGAVVKIGAVN
PKMYKHEGPARVFDSEEEAVDAILGGDIERGDVVVIRYEGPAGGPGMREMLAPTSAICGMGLDDSVALITDGRFSGGSRG
PCIGHVSPEAMAGGPIAIVEDGDIIKIDMINKKLDLALDEEEIKERLAKWKKPEPKVKKGYLARYAKLVSSADEGAVLRY
D

Sequences:

>Translated_561_residues
MNFMKREKMISDRVKKGLKRAPNRSLLKACGYTDEELERPFIGVVNSFTEVVPGHIHLRDIAEAVKKGIYANGGTAFEFN
TMAICDGIAMGHEGMKYSLPSREIIADTVESMAKAHGFDGLVLIPSCDKIVPGMIMGAIRTGLPFIVVTGGPMFPGELRG
KKYDLISVFEGVGACAAGKITEEELKEIEDIACPGAGSCAGLFTANTMACLTEAMGLSLPYCATSHATTAEKIRIAKRSG
MRIVDLVRNNITPDKILTKEAFENAILVDLALGGSTNTTLHIPAIANEVKPKFITLDDFDRLSGEVPHIASLRPGGEHFI
IDLHRAGGIPAVLKVLEEKIRKECLTVSGKTIGEIIKEVKYIDYSVIRPVDNPVHETAGLRILKGSLAPNGAVVKIGAVN
PKMYKHEGPARVFDSEEEAVDAILGGDIERGDVVVIRYEGPAGGPGMREMLAPTSAICGMGLDDSVALITDGRFSGGSRG
PCIGHVSPEAMAGGPIAIVEDGDIIKIDMINKKLDLALDEEEIKERLAKWKKPEPKVKKGYLARYAKLVSSADEGAVLRY
D
>Mature_561_residues
MNFMKREKMISDRVKKGLKRAPNRSLLKACGYTDEELERPFIGVVNSFTEVVPGHIHLRDIAEAVKKGIYANGGTAFEFN
TMAICDGIAMGHEGMKYSLPSREIIADTVESMAKAHGFDGLVLIPSCDKIVPGMIMGAIRTGLPFIVVTGGPMFPGELRG
KKYDLISVFEGVGACAAGKITEEELKEIEDIACPGAGSCAGLFTANTMACLTEAMGLSLPYCATSHATTAEKIRIAKRSG
MRIVDLVRNNITPDKILTKEAFENAILVDLALGGSTNTTLHIPAIANEVKPKFITLDDFDRLSGEVPHIASLRPGGEHFI
IDLHRAGGIPAVLKVLEEKIRKECLTVSGKTIGEIIKEVKYIDYSVIRPVDNPVHETAGLRILKGSLAPNGAVVKIGAVN
PKMYKHEGPARVFDSEEEAVDAILGGDIERGDVVVIRYEGPAGGPGMREMLAPTSAICGMGLDDSVALITDGRFSGGSRG
PCIGHVSPEAMAGGPIAIVEDGDIIKIDMINKKLDLALDEEEIKERLAKWKKPEPKVKKGYLARYAKLVSSADEGAVLRY
D

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=609, Percent_Identity=42.0361247947455, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1786464, Length=490, Percent_Identity=34.6938775510204, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788157, Length=514, Percent_Identity=31.7120622568093, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI2367371, Length=534, Percent_Identity=31.6479400749064, Blast_Score=212, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6322476, Length=551, Percent_Identity=42.2867513611615, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60264; Mature: 60264

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFMKREKMISDRVKKGLKRAPNRSLLKACGYTDEELERPFIGVVNSFTEVVPGHIHLRD
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCEEHHH
IAEAVKKGIYANGGTAFEFNTMAICDGIAMGHEGMKYSLPSREIIADTVESMAKAHGFDG
HHHHHHCCCCCCCCCEEEECHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCC
LVLIPSCDKIVPGMIMGAIRTGLPFIVVTGGPMFPGELRGKKYDLISVFEGVGACAAGKI
EEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCEEHHHHHHHHCCCHHCCCC
TEEELKEIEDIACPGAGSCAGLFTANTMACLTEAMGLSLPYCATSHATTAEKIRIAKRSG
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
MRIVDLVRNNITPDKILTKEAFENAILVDLALGGSTNTTLHIPAIANEVKPKFITLDDFD
CEEHHHHHCCCCHHHHHHHHHHCCEEEEEEEECCCCCCEEECCHHHCCCCCCEEEECCHH
RLSGEVPHIASLRPGGEHFIIDLHRAGGIPAVLKVLEEKIRKECLTVSGKTIGEIIKEVK
HHCCCCCCEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YIDYSVIRPVDNPVHETAGLRILKGSLAPNGAVVKIGAVNPKMYKHEGPARVFDSEEEAV
HCCCEEECCCCCCHHHHCCCEEEECCCCCCCCEEEEECCCCHHCCCCCCCCCCCCHHHHH
DAILGGDIERGDVVVIRYEGPAGGPGMREMLAPTSAICGMGLDDSVALITDGRFSGGSRG
HHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCC
PCIGHVSPEAMAGGPIAIVEDGDIIKIDMINKKLDLALDEEEIKERLAKWKKPEPKVKKG
CEEECCCCHHHCCCCEEEEECCCEEEEEEECCCCCCEECHHHHHHHHHHCCCCCCHHHHH
YLARYAKLVSSADEGAVLRYD
HHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MNFMKREKMISDRVKKGLKRAPNRSLLKACGYTDEELERPFIGVVNSFTEVVPGHIHLRD
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCEEHHH
IAEAVKKGIYANGGTAFEFNTMAICDGIAMGHEGMKYSLPSREIIADTVESMAKAHGFDG
HHHHHHCCCCCCCCCEEEECHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCC
LVLIPSCDKIVPGMIMGAIRTGLPFIVVTGGPMFPGELRGKKYDLISVFEGVGACAAGKI
EEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCEEHHHHHHHHCCCHHCCCC
TEEELKEIEDIACPGAGSCAGLFTANTMACLTEAMGLSLPYCATSHATTAEKIRIAKRSG
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
MRIVDLVRNNITPDKILTKEAFENAILVDLALGGSTNTTLHIPAIANEVKPKFITLDDFD
CEEHHHHHCCCCHHHHHHHHHHCCEEEEEEEECCCCCCEEECCHHHCCCCCCEEEECCHH
RLSGEVPHIASLRPGGEHFIIDLHRAGGIPAVLKVLEEKIRKECLTVSGKTIGEIIKEVK
HHCCCCCCEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YIDYSVIRPVDNPVHETAGLRILKGSLAPNGAVVKIGAVNPKMYKHEGPARVFDSEEEAV
HCCCEEECCCCCCHHHHCCCEEEECCCCCCCCEEEEECCCCHHCCCCCCCCCCCCHHHHH
DAILGGDIERGDVVVIRYEGPAGGPGMREMLAPTSAICGMGLDDSVALITDGRFSGGSRG
HHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCC
PCIGHVSPEAMAGGPIAIVEDGDIIKIDMINKKLDLALDEEEIKERLAKWKKPEPKVKKG
CEEECCCCHHHCCCCEEEEECCCEEEEEEECCCCCCEECHHHHHHHHHHCCCCCCHHHHH
YLARYAKLVSSADEGAVLRYD
HHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]