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The map label for this gene is hacB

Identifier: 15669457

GI number: 15669457

Start: 1215633

End: 1216145

Strand: Direct

Name: hacB

Synonym: MJ1271

Alternate gene names: 15669457

Gene position: 1215633-1216145 (Clockwise)

Preceding gene: 15669456

Following gene: 15669458

Centisome position: 73.01

GC content: 34.7

Gene sequence:

>513_bases
ATGATTATTAAGGGAAGAGCTCACAAATTTGGGGATGATGTAGATACAGACGCAATAATTCCAGGACCTTACTTAAGGAC
TACAGACCCTTACGAGTTAGCTTCACACTGCATGGCAGGGATAGATGAAAACTTCCCGAAAAAGGTTAAGGAGGGGGATG
TGATAGTTGCTGGAGAGAATTTTGGTTGTGGTTCAAGTAGGGAGCAGGCTGTAATAGCAATAAAATACTGTGGTATTAAG
GCTGTGATAGCAAAAAGCTTTGCAAGAATATTCTATAGAAATGCAATAAACGTTGGATTAATACCAATAATAGCAAATAC
AGATGAAATTAAAGACGGAGACATAGTAGAGATTGATTTAGATAAAGAAGAGATTGTAATAACCAATAAAAACAAAACAA
TAAAGTGTGAAACACCAAAAGGTTTAGAAAGAGAAATATTGGCTGCTGGTGGCTTAGTCAATTATTTAAAAAAGAGAAAA
CTAATACAATCAAAAAAAGGTGTAAAAACATGA

Upstream 100 bases:

>100_bases
GATACCATATTTCCTAAAGAATATTTTTATTTGAACATTTTCAGCTCTTTTGAAATTTAATTTTTAAAATTAAGATAAAA
TATTTTTGTGGTGATAAGAA

Downstream 100 bases:

>100_bases
TGTTGGAGTTAAATGGACTACATGCAGGTTTAAGGTTTTCATCAGCCCATATTGTATTTGGACATCCAACTTGTGGGGTT
ATACATGGACATTCTTATTA

Product: 3-isopropylmalate dehydratase LeuD

Products: NA

Alternate protein names: HACN; (R)-homocitrate dehydratase; Homoaconitate hydratase

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGENFGCGSSREQAVIAIKYCGIK
AVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDLDKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKRK
LIQSKKGVKT

Sequences:

>Translated_170_residues
MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGENFGCGSSREQAVIAIKYCGIK
AVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDLDKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKRK
LIQSKKGVKT
>Mature_170_residues
MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGENFGCGSSREQAVIAIKYCGIK
AVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDLDKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKRK
LIQSKKGVKT

Specific function: Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxyla

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=136, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=95, Percent_Identity=46.3157894736842, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=108, Percent_Identity=40.7407407407407, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HACB_METJA (Q58667)

Other databases:

- EMBL:   L77117
- PIR:   F64458
- RefSeq:   NP_248267.1
- PDB:   2PKP
- PDBsum:   2PKP
- ProteinModelPortal:   Q58667
- SMR:   Q58667
- GeneID:   1452169
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1271
- NMPDR:   fig|243232.1.peg.1304
- TIGR:   MJ1271
- HOGENOM:   HBG304838
- OMA:   LASHCMA
- ProtClustDB:   CLSK2749971
- BioCyc:   MJAN243232:MJ_1271-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.36

Molecular weight: Translated: 18665; Mature: 18665

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGEN
CEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
FGCGSSREQAVIAIKYCGIKAVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDL
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEC
DKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKRKLIQSKKGVKT
CCCEEEEECCCCEEEECCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGEN
CEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
FGCGSSREQAVIAIKYCGIKAVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDL
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEC
DKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKRKLIQSKKGVKT
CCCEEEEECCCCEEEECCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087