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The map label for this gene is livM

Identifier: 15669456

GI number: 15669456

Start: 1214390

End: 1215427

Strand: Direct

Name: livM

Synonym: MJ1270

Alternate gene names: 15669456

Gene position: 1214390-1215427 (Clockwise)

Preceding gene: 15669455

Following gene: 15669457

Centisome position: 72.94

GC content: 31.12

Gene sequence:

>1038_bases
ATGAGTATTGATTTAATATCCATGATTTTATTGTGGTTTGGGCTTTATTATATTGTTTCTCTTTCCTTAAATATGGAATT
TGGTTATGCAGGAATTCCAAACTTTGGTAAGGCATTATCAGTTTTGGTTGGAGCTATTGCAGTTGGGGGAATTTTAGATA
GGTTGTTAATGCTATATTTCGGTATTGGAGGAGATTTTATCACTGGAACTACTTATGCAACATCTGCTATAAACAATTTA
ATTGCCTCAAATCCAATAGTTGGGATTGGAATATTAATATTAGCAATAATATTGGCTTCAATTCTTGGTTTTGTAGTTGG
GGCAATCTTTATCTTACCAAGTGCTAAATTAAAGGAGGACTATTTAGGAATTACCTTATTAGCTATAAGTGAAGCAGTTC
TTTTAATCTGCACATATAACTTAAATATAATTGGTGGTTATTATGGAATTTCAACCCCAGATATATTAGCATTTGTCTCT
GGAGAATATAGAGGTTGGGTATTTGCATGGATTGTATTATTTATTGCTTTCTTAGTTTATTTATTCTTTGAAAGATTGTT
GAATACTCCATTTGGTAGAGTATTAAGGGCTATGAGAGAAAATGAAAATACAGTTAAAGCATTTGGTAGAGATATAATGA
AATTAAGAATAAAAACAATGGCAATTGGTTCTGCAATTGGAGCAATTGCAGGGGTTTTGTATTCATTATATACTGTAAAT
ATCATTGCTAATGCATTTACAAGAGTAGATTGGACTTTCTTCCCATTTTTAATGGTATTATTGGGAGGAAAGGGAAATAA
CAAAGGAGTTGCTTTAGGGGTTTTATGTTATGTGATAGTTAAGGTCTTATTAGATATATACAAATATAATATAAAATATG
CATTAGGTATTCCATTTGAGCCCGTCTGGCTTTCATATATGTTATTTGGAGTTTTAATGCTTCTTATCCTCTACTACAAG
CCATCTGGCTTAATTCCAGAGAAACCAATTATAACTCCACCAATGAAAAAGAAAATCATGGAAATTAGTGGCAAATAA

Upstream 100 bases:

>100_bases
AGCAACATTATTAATAGCACCTTATGGAATTACTGGAGTCGATTGGAAAAAGTTAAAAAGATTATTATCTACTTCATAAT
TTAACTTATGGGGTGAAAAT

Downstream 100 bases:

>100_bases
CTAAATATAATAGAATTCTCCCGAGAATAAATGTTTTTATCATTCTGAAGTAAATATATAGAAACTCCTATAATACCCAT
TAATAAATTTTATTAATTTC

Product: high-affinity branched-chain amino acid transport protein BraE

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSIDLISMILLWFGLYYIVSLSLNMEFGYAGIPNFGKALSVLVGAIAVGGILDRLLMLYFGIGGDFITGTTYATSAINNL
IASNPIVGIGILILAIILASILGFVVGAIFILPSAKLKEDYLGITLLAISEAVLLICTYNLNIIGGYYGISTPDILAFVS
GEYRGWVFAWIVLFIAFLVYLFFERLLNTPFGRVLRAMRENENTVKAFGRDIMKLRIKTMAIGSAIGAIAGVLYSLYTVN
IIANAFTRVDWTFFPFLMVLLGGKGNNKGVALGVLCYVIVKVLLDIYKYNIKYALGIPFEPVWLSYMLFGVLMLLILYYK
PSGLIPEKPIITPPMKKKIMEISGK

Sequences:

>Translated_345_residues
MSIDLISMILLWFGLYYIVSLSLNMEFGYAGIPNFGKALSVLVGAIAVGGILDRLLMLYFGIGGDFITGTTYATSAINNL
IASNPIVGIGILILAIILASILGFVVGAIFILPSAKLKEDYLGITLLAISEAVLLICTYNLNIIGGYYGISTPDILAFVS
GEYRGWVFAWIVLFIAFLVYLFFERLLNTPFGRVLRAMRENENTVKAFGRDIMKLRIKTMAIGSAIGAIAGVLYSLYTVN
IIANAFTRVDWTFFPFLMVLLGGKGNNKGVALGVLCYVIVKVLLDIYKYNIKYALGIPFEPVWLSYMLFGVLMLLILYYK
PSGLIPEKPIITPPMKKKIMEISGK
>Mature_344_residues
SIDLISMILLWFGLYYIVSLSLNMEFGYAGIPNFGKALSVLVGAIAVGGILDRLLMLYFGIGGDFITGTTYATSAINNLI
ASNPIVGIGILILAIILASILGFVVGAIFILPSAKLKEDYLGITLLAISEAVLLICTYNLNIIGGYYGISTPDILAFVSG
EYRGWVFAWIVLFIAFLVYLFFERLLNTPFGRVLRAMRENENTVKAFGRDIMKLRIKTMAIGSAIGAIAGVLYSLYTVNI
IANAFTRVDWTFFPFLMVLLGGKGNNKGVALGVLCYVIVKVLLDIYKYNIKYALGIPFEPVWLSYMLFGVLMLLILYYKP
SGLIPEKPIITPPMKKKIMEISGK

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily

Homologues:

Organism=Escherichia coli, GI1789865, Length=362, Percent_Identity=25.1381215469613, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIVM_METJA (Q58666)

Other databases:

- EMBL:   L77117
- PIR:   E64458
- RefSeq:   NP_248266.1
- ProteinModelPortal:   Q58666
- GeneID:   1452168
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1270
- NMPDR:   fig|243232.1.peg.1303
- TIGR:   MJ1270
- HOGENOM:   HBG655109
- OMA:   DYLAITL
- ProtClustDB:   CLSK541725
- BioCyc:   MJAN243232:MJ_1270-MONOMER
- InterPro:   IPR001851

Pfam domain/function: PF02653 BPD_transp_2

EC number: NA

Molecular weight: Translated: 37937; Mature: 37806

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe3379a4)-; HASH(0xe6a9490)-; HASH(0xdf64698)-; HASH(0xdbb589c)-; HASH(0xb362ebc)-; HASH(0xe8ed5dc)-; HASH(0xe709014)-; HASH(0xe4c0e40)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDLISMILLWFGLYYIVSLSLNMEFGYAGIPNFGKALSVLVGAIAVGGILDRLLMLYF
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GIGGDFITGTTYATSAINNLIASNPIVGIGILILAIILASILGFVVGAIFILPSAKLKED
CCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YLGITLLAISEAVLLICTYNLNIIGGYYGISTPDILAFVSGEYRGWVFAWIVLFIAFLVY
HHHHHHHHHHHHHHHHEEECCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
LFFERLLNTPFGRVLRAMRENENTVKAFGRDIMKLRIKTMAIGSAIGAIAGVLYSLYTVN
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIANAFTRVDWTFFPFLMVLLGGKGNNKGVALGVLCYVIVKVLLDIYKYNIKYALGIPFE
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
PVWLSYMLFGVLMLLILYYKPSGLIPEKPIITPPMKKKIMEISGK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCH
>Mature Secondary Structure 
SIDLISMILLWFGLYYIVSLSLNMEFGYAGIPNFGKALSVLVGAIAVGGILDRLLMLYF
CHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GIGGDFITGTTYATSAINNLIASNPIVGIGILILAIILASILGFVVGAIFILPSAKLKED
CCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YLGITLLAISEAVLLICTYNLNIIGGYYGISTPDILAFVSGEYRGWVFAWIVLFIAFLVY
HHHHHHHHHHHHHHHHEEECCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
LFFERLLNTPFGRVLRAMRENENTVKAFGRDIMKLRIKTMAIGSAIGAIAGVLYSLYTVN
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIANAFTRVDWTFFPFLMVLLGGKGNNKGVALGVLCYVIVKVLLDIYKYNIKYALGIPFE
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
PVWLSYMLFGVLMLLILYYKPSGLIPEKPIITPPMKKKIMEISGK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087