Click here to switch to the map view.

The map label for this gene is ndk

Identifier: 15669451

GI number: 15669451

Start: 1209919

End: 1210341

Strand: Reverse

Name: ndk

Synonym: MJ1265

Alternate gene names: 15669451

Gene position: 1210341-1209919 (Counterclockwise)

Preceding gene: 15669460

Following gene: 15669450

Centisome position: 72.69

GC content: 30.73

Gene sequence:

>423_bases
ATGAAAGAAAGAACCTTTGTAGCTTTAAAACCAGATGCTGTAAAAAGAAAACTAATTGGAAAAATCATTGAAAGATTTGA
AAATAAAGGTTTTGAGATTGTGGCTATGAAGATGATTAAATTAGATAGAGAGATGGCAGAAAAATATTATGAAGAGCATA
AAGGGAAAGAATTTTATGAGAGACTAATAAACTTTATGACATCTGGAAGAATGATAGTTATGGTTGTTGAGGGAGAAAAT
GCCATATCTGTTGTAAGAAAGATGATTGGTAAAACAAATCCTGCTGAAGCAGAACCAGGAACTATAAGAGGAGATTTTGC
TTTAACAACCCCGGATAATATAATTCATGCATCAGATTCAAAGGAAAGTGCTGAAAGAGAGATAAAACTATTTTTTAAAG
AGGATGAGATATTTGATAAATAA

Upstream 100 bases:

>100_bases
CCAACAATATTATCATTATTTTTAATAACTTCCGTATCAAAAAGCTTTAAAGTATTAGTAATAATAATAGTAATTATATA
ATATCAAAAGCGGGATAGTT

Downstream 100 bases:

>100_bases
GCATAAAGGTTTAATAACACATCCTTCATTTTTTGTATATCCACAAATAATATTAATTAACTAATAAGGTGTAATTATGG
ATGAGAATGATTTAAAGTAT

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MKERTFVALKPDAVKRKLIGKIIERFENKGFEIVAMKMIKLDREMAEKYYEEHKGKEFYERLINFMTSGRMIVMVVEGEN
AISVVRKMIGKTNPAEAEPGTIRGDFALTTPDNIIHASDSKESAEREIKLFFKEDEIFDK

Sequences:

>Translated_140_residues
MKERTFVALKPDAVKRKLIGKIIERFENKGFEIVAMKMIKLDREMAEKYYEEHKGKEFYERLINFMTSGRMIVMVVEGEN
AISVVRKMIGKTNPAEAEPGTIRGDFALTTPDNIIHASDSKESAEREIKLFFKEDEIFDK
>Mature_140_residues
MKERTFVALKPDAVKRKLIGKIIERFENKGFEIVAMKMIKLDREMAEKYYEEHKGKEFYERLINFMTSGRMIVMVVEGEN
AISVVRKMIGKTNPAEAEPGTIRGDFALTTPDNIIHASDSKESAEREIKLFFKEDEIFDK

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family

Homologues:

Organism=Homo sapiens, GI37693993, Length=136, Percent_Identity=49.2647058823529, Blast_Score=157, Evalue=4e-39,
Organism=Homo sapiens, GI4826862, Length=136, Percent_Identity=49.2647058823529, Blast_Score=154, Evalue=2e-38,
Organism=Homo sapiens, GI66392203, Length=137, Percent_Identity=50.3649635036496, Blast_Score=152, Evalue=1e-37,
Organism=Homo sapiens, GI66392227, Length=137, Percent_Identity=50.3649635036496, Blast_Score=152, Evalue=1e-37,
Organism=Homo sapiens, GI66392205, Length=137, Percent_Identity=50.3649635036496, Blast_Score=152, Evalue=1e-37,
Organism=Homo sapiens, GI4505409, Length=137, Percent_Identity=50.3649635036496, Blast_Score=152, Evalue=1e-37,
Organism=Homo sapiens, GI66392192, Length=137, Percent_Identity=50.3649635036496, Blast_Score=152, Evalue=1e-37,
Organism=Homo sapiens, GI38045913, Length=137, Percent_Identity=48.1751824817518, Blast_Score=149, Evalue=9e-37,
Organism=Homo sapiens, GI4557797, Length=137, Percent_Identity=48.1751824817518, Blast_Score=148, Evalue=1e-36,
Organism=Homo sapiens, GI5031951, Length=135, Percent_Identity=38.5185185185185, Blast_Score=93, Evalue=8e-20,
Organism=Homo sapiens, GI7019465, Length=133, Percent_Identity=36.0902255639098, Blast_Score=86, Evalue=7e-18,
Organism=Homo sapiens, GI37574614, Length=133, Percent_Identity=36.0902255639098, Blast_Score=86, Evalue=8e-18,
Organism=Homo sapiens, GI4505413, Length=136, Percent_Identity=33.0882352941176, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI148839372, Length=135, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI30023828, Length=143, Percent_Identity=29.3706293706294, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1788866, Length=135, Percent_Identity=51.1111111111111, Blast_Score=144, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17506807, Length=137, Percent_Identity=47.4452554744526, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71995094, Length=130, Percent_Identity=36.9230769230769, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322783, Length=137, Percent_Identity=47.4452554744526, Blast_Score=152, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45549037, Length=136, Percent_Identity=52.2058823529412, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18860097, Length=135, Percent_Identity=32.5925925925926, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDK_METJA (Q58661)

Other databases:

- EMBL:   L77117
- PIR:   H64457
- RefSeq:   NP_248261.1
- ProteinModelPortal:   Q58661
- SMR:   Q58661
- GeneID:   1452163
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1265
- NMPDR:   fig|243232.1.peg.1298
- TIGR:   MJ1265
- HOGENOM:   HBG445152
- OMA:   HEMPIFF
- ProtClustDB:   PRK14540
- BioCyc:   MJAN243232:MJ_1265-MONOMER
- BRENDA:   2.7.4.6
- GO:   GO:0005737
- HAMAP:   MF_00451
- InterPro:   IPR001564
- InterPro:   IPR023005
- Gene3D:   G3DSA:3.30.70.141
- PANTHER:   PTHR11349
- PRINTS:   PR01243
- SMART:   SM00562

Pfam domain/function: PF00334 NDK; SSF54919 NDK

EC number: =2.7.4.6

Molecular weight: Translated: 16224; Mature: 16224

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00469 NDP_KINASES

Important sites: ACT_SITE 116-116 BINDING 10-10 BINDING 58-58 BINDING 86-86 BINDING 92-92 BINDING 103-103 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERTFVALKPDAVKRKLIGKIIERFENKGFEIVAMKMIKLDREMAEKYYEEHKGKEFYE
CCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RLINFMTSGRMIVMVVEGENAISVVRKMIGKTNPAEAEPGTIRGDFALTTPDNIIHASDS
HHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEECCCC
KESAEREIKLFFKEDEIFDK
HHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MKERTFVALKPDAVKRKLIGKIIERFENKGFEIVAMKMIKLDREMAEKYYEEHKGKEFYE
CCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RLINFMTSGRMIVMVVEGENAISVVRKMIGKTNPAEAEPGTIRGDFALTTPDNIIHASDS
HHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEECCCC
KESAEREIKLFFKEDEIFDK
HHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 11053861