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The map label for this gene is metG

Identifier: 15669449

GI number: 15669449

Start: 1205661

End: 1207616

Strand: Direct

Name: metG

Synonym: MJ1263

Alternate gene names: 15669449

Gene position: 1205661-1207616 (Clockwise)

Preceding gene: 15669448

Following gene: 15669452

Centisome position: 72.41

GC content: 33.38

Gene sequence:

>1956_bases
ATGAGATATCTAATAACAACTGCCTTAGCTTATACAAACGGCCCTCTACATTTAGGGCATGCAAGAAGCACTTACATCCC
AGCAGATATTATATATAAATACTTAAAGTTGAGAGGGGAAGATGTTATCCACGTTGGAGGAACTGATAACCACGGAGTTC
CTATAACATTAACTGCTGAAAAAGAGGGAAAAAGCCCAGAGGAGATTGTTGAAAAATACCATAATGAGATTAAAGAAGAT
TTAGATTTGTTAGGAGTAGAGTTTGATGCATTTGGCAAAACTCACAGCCAAATACATATAGAAACAGCTCAAGAGTTTTA
TCTAAAGCTAAAAGAGAATGGCTATATCTATGAGAAGGAAATAGAGCAATTTTACTGTCCAAACTGTAAAAAATTCTTAC
CAGATAGATATGTTGAGGGAATCTGCCCATACTGTGGAGGAGAGGCAAGAGGAGACCACTGTGAAGTTTGTGGAAGACAC
TTAGAGCCGTTTGAGTTAAAAGACCCATATTGTGTAATTTGCAAAGGAAAGCCAGAGATTAGAAAGACAAAACATCACTT
CTTTAAGTTAAGTGCTTTAAAAAAGGAGTTAGAGGAGTATATAAAAAATGCAAAAGAAATGCCAGAGCATGTTAAAAATA
TGGCATTGAATTGGATTAAAGAGTTACATGATTGGGATATTTCAAGAGATATAAGCTGGGGAGTTCCAATTCCAGGGACT
AATCAAGTAATGTATGTTTGGTTAGAAGCCCCTATTGGCTATATCTCATTTACAAAGATGTTGGGAGAGATTTGGAAGAA
ATATTGGTTAGAGAAAGATACAAAGATTTATCACTTCATTGGAAAGGATATAACTGTTCATCATGCCGTTTTCTGGCCAG
GAATGTTGATTGCTCATGGTTCCTTTAACTTACCAACTGCAGTAGTTAGTGGAGGTTATCTAACCTTAGAAGGAAGAAAG
ATGAGCACAAGTAAAAGATGGGTTGTTTGGGTTAAAGATTTTGTTAAAAACTTTGATGCTGACTATTTAAGATACTACTT
AATCATGTCAGCCCCTCTCTTTAAAGATTGTGATTTCTCATTTGATGATTTCAAAAATAAGATAAACAATGAACTCATCA
ACATAATTGGAAACTTCACCCACAGAGTTTTAACCTTTACCCATAGAAAGTTTAAGAAAGTCCCAATAGTTGATGAAGAT
AGATTAAAAGAGGAAGATAAAGAGCTATTGAAAAAATGTGAAGAGACACTTGAGGCTGTGGATAAAAACATAAGGAGCTT
TAAGTTTAGAGATGCTTTAGTTAATATATTACATCTTGCCATTGAAGGAAACAGTTACTTCCAAAAGATGGAGCCTTGGG
CTGTTGATGATGAAGAAAGATTGAAGGAAATATTATATACTTGCTGCAAGACAGTAAAAACTCTTGTCTATCTGCTATAC
CCATACATGCCTAAAAAATCTCTTGCACTATTGGAATTAATGAATGAAGAACTTGATTTAGAGCTTAGAGGGAATGAATT
AAAGAAACCAAAGATTATATTCAAAAAGATAGATAATAAGAAGATAGAAGAGATGAAGAAAAAGCTTTATGAAAATAAAA
AAGAAGAAACAAAAGGAGGAGAAAAAATGGAGCAAATAGATATAAGTTATTTAGAAAAGATTGATTTAAGAGTTGGAGAA
GTTGTAGAGGCAGAAGACATACCAAAATCAAAGAAACTTTTAAAACTAATGGTTGATTTGGGAGATGAGAAGAGACAGAT
TGTTTCAGGAATTAAAGGATATTATAAACCTGAAGATTTAGTTGGAAAAAAGGTTATTGTTATTTGCAATTTAAAACCAG
CTAAATTATGTGGTGTTTTATCTGAAGGAATGATTTTAGCAGCCGAAGATGATGAAGGAAATGTTAGCTTATTAACTGTT
GATAAGGATATAAAAGCAGGTAGTAAAGTTAGATAA

Upstream 100 bases:

>100_bases
AAGGAAATTTGAATGCCTTCTATAAGAAGGCGTTCATTAATACATTAATAGTTATTACAAAATATTTTGAAAGACACTAT
TAGTCAAAAGGAGGGAGAGG

Downstream 100 bases:

>100_bases
ACTAAAAAAATAAGAATATTTAATCTAAAGCTCTCCCATCATCTTTGGAAATGCTTCATAATACCTCTTTTTCATCTCTT
CCACATCCAAATTAACAACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MRYLITTALAYTNGPLHLGHARSTYIPADIIYKYLKLRGEDVIHVGGTDNHGVPITLTAEKEGKSPEEIVEKYHNEIKED
LDLLGVEFDAFGKTHSQIHIETAQEFYLKLKENGYIYEKEIEQFYCPNCKKFLPDRYVEGICPYCGGEARGDHCEVCGRH
LEPFELKDPYCVICKGKPEIRKTKHHFFKLSALKKELEEYIKNAKEMPEHVKNMALNWIKELHDWDISRDISWGVPIPGT
NQVMYVWLEAPIGYISFTKMLGEIWKKYWLEKDTKIYHFIGKDITVHHAVFWPGMLIAHGSFNLPTAVVSGGYLTLEGRK
MSTSKRWVVWVKDFVKNFDADYLRYYLIMSAPLFKDCDFSFDDFKNKINNELINIIGNFTHRVLTFTHRKFKKVPIVDED
RLKEEDKELLKKCEETLEAVDKNIRSFKFRDALVNILHLAIEGNSYFQKMEPWAVDDEERLKEILYTCCKTVKTLVYLLY
PYMPKKSLALLELMNEELDLELRGNELKKPKIIFKKIDNKKIEEMKKKLYENKKEETKGGEKMEQIDISYLEKIDLRVGE
VVEAEDIPKSKKLLKLMVDLGDEKRQIVSGIKGYYKPEDLVGKKVIVICNLKPAKLCGVLSEGMILAAEDDEGNVSLLTV
DKDIKAGSKVR

Sequences:

>Translated_651_residues
MRYLITTALAYTNGPLHLGHARSTYIPADIIYKYLKLRGEDVIHVGGTDNHGVPITLTAEKEGKSPEEIVEKYHNEIKED
LDLLGVEFDAFGKTHSQIHIETAQEFYLKLKENGYIYEKEIEQFYCPNCKKFLPDRYVEGICPYCGGEARGDHCEVCGRH
LEPFELKDPYCVICKGKPEIRKTKHHFFKLSALKKELEEYIKNAKEMPEHVKNMALNWIKELHDWDISRDISWGVPIPGT
NQVMYVWLEAPIGYISFTKMLGEIWKKYWLEKDTKIYHFIGKDITVHHAVFWPGMLIAHGSFNLPTAVVSGGYLTLEGRK
MSTSKRWVVWVKDFVKNFDADYLRYYLIMSAPLFKDCDFSFDDFKNKINNELINIIGNFTHRVLTFTHRKFKKVPIVDED
RLKEEDKELLKKCEETLEAVDKNIRSFKFRDALVNILHLAIEGNSYFQKMEPWAVDDEERLKEILYTCCKTVKTLVYLLY
PYMPKKSLALLELMNEELDLELRGNELKKPKIIFKKIDNKKIEEMKKKLYENKKEETKGGEKMEQIDISYLEKIDLRVGE
VVEAEDIPKSKKLLKLMVDLGDEKRQIVSGIKGYYKPEDLVGKKVIVICNLKPAKLCGVLSEGMILAAEDDEGNVSLLTV
DKDIKAGSKVR
>Mature_651_residues
MRYLITTALAYTNGPLHLGHARSTYIPADIIYKYLKLRGEDVIHVGGTDNHGVPITLTAEKEGKSPEEIVEKYHNEIKED
LDLLGVEFDAFGKTHSQIHIETAQEFYLKLKENGYIYEKEIEQFYCPNCKKFLPDRYVEGICPYCGGEARGDHCEVCGRH
LEPFELKDPYCVICKGKPEIRKTKHHFFKLSALKKELEEYIKNAKEMPEHVKNMALNWIKELHDWDISRDISWGVPIPGT
NQVMYVWLEAPIGYISFTKMLGEIWKKYWLEKDTKIYHFIGKDITVHHAVFWPGMLIAHGSFNLPTAVVSGGYLTLEGRK
MSTSKRWVVWVKDFVKNFDADYLRYYLIMSAPLFKDCDFSFDDFKNKINNELINIIGNFTHRVLTFTHRKFKKVPIVDED
RLKEEDKELLKKCEETLEAVDKNIRSFKFRDALVNILHLAIEGNSYFQKMEPWAVDDEERLKEILYTCCKTVKTLVYLLY
PYMPKKSLALLELMNEELDLELRGNELKKPKIIFKKIDNKKIEEMKKKLYENKKEETKGGEKMEQIDISYLEKIDLRVGE
VVEAEDIPKSKKLLKLMVDLGDEKRQIVSGIKGYYKPEDLVGKKVIVICNLKPAKLCGVLSEGMILAAEDDEGNVSLLTV
DKDIKAGSKVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=600, Percent_Identity=30.1666666666667, Blast_Score=296, Evalue=5e-80,
Organism=Homo sapiens, GI24308436, Length=525, Percent_Identity=25.1428571428571, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1788432, Length=685, Percent_Identity=33.4306569343066, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=39.4495412844037, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786861, Length=233, Percent_Identity=24.4635193133047, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=571, Percent_Identity=33.9754816112084, Blast_Score=319, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI212645096, Length=495, Percent_Identity=27.4747474747475, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71992781, Length=391, Percent_Identity=28.9002557544757, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321704, Length=548, Percent_Identity=31.3868613138686, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=25.0460405156538, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24655311, Length=621, Percent_Identity=31.7230273752013, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=24.1842610364683, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21357223, Length=100, Percent_Identity=38, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_METJA (Q58659)

Other databases:

- EMBL:   L77117
- PIR:   F64457
- RefSeq:   NP_248259.1
- ProteinModelPortal:   Q58659
- SMR:   Q58659
- GeneID:   1452161
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1263
- NMPDR:   fig|243232.1.peg.1296
- TIGR:   MJ1263
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- ProtClustDB:   PRK00133
- BioCyc:   MJAN243232:MJ_1263-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75560; Mature: 75560

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLITTALAYTNGPLHLGHARSTYIPADIIYKYLKLRGEDVIHVGGTDNHGVPITLTAE
CCEEEEEHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEC
KEGKSPEEIVEKYHNEIKEDLDLLGVEFDAFGKTHSQIHIETAQEFYLKLKENGYIYEKE
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEEHHHHHHEECCCCEEEHHH
IEQFYCPNCKKFLPDRYVEGICPYCGGEARGDHCEVCGRHLEPFELKDPYCVICKGKPEI
HHHHHCCCHHHHCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCHH
RKTKHHFFKLSALKKELEEYIKNAKEMPEHVKNMALNWIKELHDWDISRDISWGVPIPGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NQVMYVWLEAPIGYISFTKMLGEIWKKYWLEKDTKIYHFIGKDITVHHAVFWPGMLIAHG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHHHCCCEEEECC
SFNLPTAVVSGGYLTLEGRKMSTSKRWVVWVKDFVKNFDADYLRYYLIMSAPLFKDCDFS
CCCCCHHHHCCCEEEEECCEECCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
FDDFKNKINNELINIIGNFTHRVLTFTHRKFKKVPIVDEDRLKEEDKELLKKCEETLEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKNIRSFKFRDALVNILHLAIEGNSYFQKMEPWAVDDEERLKEILYTCCKTVKTLVYLLY
HHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PYMPKKSLALLELMNEELDLELRGNELKKPKIIFKKIDNKKIEEMKKKLYENKKEETKGG
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCHHHHCCC
EKMEQIDISYLEKIDLRVGEVVEAEDIPKSKKLLKLMVDLGDEKRQIVSGIKGYYKPEDL
CHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
VGKKVIVICNLKPAKLCGVLSEGMILAAEDDEGNVSLLTVDKDIKAGSKVR
CCCEEEEEECCCHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MRYLITTALAYTNGPLHLGHARSTYIPADIIYKYLKLRGEDVIHVGGTDNHGVPITLTAE
CCEEEEEHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEC
KEGKSPEEIVEKYHNEIKEDLDLLGVEFDAFGKTHSQIHIETAQEFYLKLKENGYIYEKE
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEEHHHHHHEECCCCEEEHHH
IEQFYCPNCKKFLPDRYVEGICPYCGGEARGDHCEVCGRHLEPFELKDPYCVICKGKPEI
HHHHHCCCHHHHCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCHH
RKTKHHFFKLSALKKELEEYIKNAKEMPEHVKNMALNWIKELHDWDISRDISWGVPIPGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NQVMYVWLEAPIGYISFTKMLGEIWKKYWLEKDTKIYHFIGKDITVHHAVFWPGMLIAHG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHHHCCCEEEECC
SFNLPTAVVSGGYLTLEGRKMSTSKRWVVWVKDFVKNFDADYLRYYLIMSAPLFKDCDFS
CCCCCHHHHCCCEEEEECCEECCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
FDDFKNKINNELINIIGNFTHRVLTFTHRKFKKVPIVDEDRLKEEDKELLKKCEETLEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKNIRSFKFRDALVNILHLAIEGNSYFQKMEPWAVDDEERLKEILYTCCKTVKTLVYLLY
HHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PYMPKKSLALLELMNEELDLELRGNELKKPKIIFKKIDNKKIEEMKKKLYENKKEETKGG
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCHHHHCCC
EKMEQIDISYLEKIDLRVGEVVEAEDIPKSKKLLKLMVDLGDEKRQIVSGIKGYYKPEDL
CHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
VGKKVIVICNLKPAKLCGVLSEGMILAAEDDEGNVSLLTVDKDIKAGSKVR
CCCEEEEEECCCHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087