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The map label for this gene is pycB

Identifier: 15669416

GI number: 15669416

Start: 1173482

End: 1175185

Strand: Reverse

Name: pycB

Synonym: MJ1231

Alternate gene names: 15669416

Gene position: 1175185-1173482 (Counterclockwise)

Preceding gene: 15669417

Following gene: 15669415

Centisome position: 70.58

GC content: 37.03

Gene sequence:

>1704_bases
ATGGTTAAGATTGTAGATACTACTTTTAGGGATGCTCAGCAGTCATTGATAGCTACAAGAATGAGAACTGAAGACATGCT
ACCAATAGCGGAAAAGATGGATGAGGTTGGATTCTACTCTATGGAGGTTTGGGGAGGAGCTACATTTGATGCATGTATAA
GATATCTAAATGAAGACCCATGGGAGAGGTTGAGGGCTTTAAAAAAGAGGATTCAAAACACTCCATTACAGATGCTCTTA
AGAGGGCAGAACTTAGTTGGTTATAGGCACTACCCAGATGATATCGTTGAAAAGTTTGTTATAAAAGCCCATGAGAATGG
AATTGATATTTTTAGGATTTTTGATGCTTTAAACGATGTAAGAAATATGGAAACTGCAATAAAAACAGCTAAAAAGGTTG
GGGCTGAAGTTCAGGGGGCTATATGTTACACTATAAGCCCAGTTCATACAATTGACCAATATGTGGAGTTAGCAAAAAAA
TTAGAAGAGATGGGGTGTGATTCAATCTGCATAAAAGATATGGCTGGGCTTTTAACCCCTTATGAAGGATATGAGTTAGT
TAAAAGATTAAAAGAAGAGATATCACTTCCTATTGACGTACATAGCCATTGCACAAGTGGTTTAGCTCCAATGACTTACC
TAAAAGTTATAGAAGCTGGAGCTGACATGGTAGATTGTGCTATCTCACCATTTGCCATGGGGACATCCCAACCACCAACA
GAGAGTATCGTTGTTGCGTTAAAAGGAACAAAATATGATACTGGCTTAGATTTAAAGCTCTTAAATGAGATTAGAGATTA
CTTCATGAAAGTTAGAGAAAAATATAAAATGCTATTCTCTCCAATATCCCAAATTGTCGATGCAAGGGTTTTGGTGTATC
AAGTTCCTGGAGGAATGCTATCTAACTTGGTCTCACAACTTAAAGAGCAGGGAGCTTTGGATAAATTTGAAGAAGTTCTA
CAGGAGATTCCAAGAGTAAGAAAGGATTTAGGATATCCTCCATTAGTTACACCAACCTCTCAAATTGTTGGAACTCAGGC
TGTTTTAAACGTTTTAACTGAAGAGAGATACAAGATTATAACAAACGAAGTAGTTAATTATGTAAAGGGCTTTTATGGAA
AGCCACCAGCTCCAATTAACCCAGAGTTGTTAAAGAGAGTATTGGATGAGGGAGAGAAACCAATTACCTGCAGACCAGCT
GATTTATTACCTCCAGAATGGGAGAAAGTTAAGAAAGAGGCAGAAGAGAAGGGAATTGTTAAGAAAGAAGAGGATATATT
AACCTACGCTTTATATCCACAGATAGCTGTTAAGTTCTTAAGAGGAGAGTTGAAAGCTGAGCCAATACCAAAAGAGAAGG
ATATAGGAAAGATTTTAGAGATTCCGACTGAATATATTGTAGAGGTTGATGGAGAGAAGTTTGAGGTTAAGATAGAGCCA
AAGATTGGAACAGAATTGAAGAGAAAGAAAGAAGTTATAACTGCAGAGATGGAGGGAGCTGTTACTTCACCATTTAGAGG
AATGGTAACTAAGATTAAAGTTAAAGAAGGAGATAAGGTTAAGAAGGGGGATGTTATTGTTGTATTAGAAGCTATGAAGA
TGGAGCATCCAATAGAAAGCCCAGTTGAGGGAACTGTAGAGAGAATATTAATTGATGAAGGAGATGCTGTGAATGTTGGA
GATGTAATTATGATTATTAAATAA

Upstream 100 bases:

>100_bases
GAAATTATAAGACTATGGCTAACCAAATAACGGAACTGCATTGCCGTTTTATTTGGCTGTGCCTTTTTTAAATCAAAATA
AATATTTGTGAGGGATAGCT

Downstream 100 bases:

>100_bases
ACTTCTCTTTTTTTGTGATATTTTGGTGATATTTATGGAAAAAAGATGGAAATGTCCAAAATGTGGAAATACAGAATTTT
TTGAAAAAGAAGTTGCAATG

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MVKIVDTTFRDAQQSLIATRMRTEDMLPIAEKMDEVGFYSMEVWGGATFDACIRYLNEDPWERLRALKKRIQNTPLQMLL
RGQNLVGYRHYPDDIVEKFVIKAHENGIDIFRIFDALNDVRNMETAIKTAKKVGAEVQGAICYTISPVHTIDQYVELAKK
LEEMGCDSICIKDMAGLLTPYEGYELVKRLKEEISLPIDVHSHCTSGLAPMTYLKVIEAGADMVDCAISPFAMGTSQPPT
ESIVVALKGTKYDTGLDLKLLNEIRDYFMKVREKYKMLFSPISQIVDARVLVYQVPGGMLSNLVSQLKEQGALDKFEEVL
QEIPRVRKDLGYPPLVTPTSQIVGTQAVLNVLTEERYKIITNEVVNYVKGFYGKPPAPINPELLKRVLDEGEKPITCRPA
DLLPPEWEKVKKEAEEKGIVKKEEDILTYALYPQIAVKFLRGELKAEPIPKEKDIGKILEIPTEYIVEVDGEKFEVKIEP
KIGTELKRKKEVITAEMEGAVTSPFRGMVTKIKVKEGDKVKKGDVIVVLEAMKMEHPIESPVEGTVERILIDEGDAVNVG
DVIMIIK

Sequences:

>Translated_567_residues
MVKIVDTTFRDAQQSLIATRMRTEDMLPIAEKMDEVGFYSMEVWGGATFDACIRYLNEDPWERLRALKKRIQNTPLQMLL
RGQNLVGYRHYPDDIVEKFVIKAHENGIDIFRIFDALNDVRNMETAIKTAKKVGAEVQGAICYTISPVHTIDQYVELAKK
LEEMGCDSICIKDMAGLLTPYEGYELVKRLKEEISLPIDVHSHCTSGLAPMTYLKVIEAGADMVDCAISPFAMGTSQPPT
ESIVVALKGTKYDTGLDLKLLNEIRDYFMKVREKYKMLFSPISQIVDARVLVYQVPGGMLSNLVSQLKEQGALDKFEEVL
QEIPRVRKDLGYPPLVTPTSQIVGTQAVLNVLTEERYKIITNEVVNYVKGFYGKPPAPINPELLKRVLDEGEKPITCRPA
DLLPPEWEKVKKEAEEKGIVKKEEDILTYALYPQIAVKFLRGELKAEPIPKEKDIGKILEIPTEYIVEVDGEKFEVKIEP
KIGTELKRKKEVITAEMEGAVTSPFRGMVTKIKVKEGDKVKKGDVIVVLEAMKMEHPIESPVEGTVERILIDEGDAVNVG
DVIMIIK
>Mature_567_residues
MVKIVDTTFRDAQQSLIATRMRTEDMLPIAEKMDEVGFYSMEVWGGATFDACIRYLNEDPWERLRALKKRIQNTPLQMLL
RGQNLVGYRHYPDDIVEKFVIKAHENGIDIFRIFDALNDVRNMETAIKTAKKVGAEVQGAICYTISPVHTIDQYVELAKK
LEEMGCDSICIKDMAGLLTPYEGYELVKRLKEEISLPIDVHSHCTSGLAPMTYLKVIEAGADMVDCAISPFAMGTSQPPT
ESIVVALKGTKYDTGLDLKLLNEIRDYFMKVREKYKMLFSPISQIVDARVLVYQVPGGMLSNLVSQLKEQGALDKFEEVL
QEIPRVRKDLGYPPLVTPTSQIVGTQAVLNVLTEERYKIITNEVVNYVKGFYGKPPAPINPELLKRVLDEGEKPITCRPA
DLLPPEWEKVKKEAEEKGIVKKEEDILTYALYPQIAVKFLRGELKAEPIPKEKDIGKILEIPTEYIVEVDGEKFEVKIEP
KIGTELKRKKEVITAEMEGAVTSPFRGMVTKIKVKEGDKVKKGDVIVVLEAMKMEHPIESPVEGTVERILIDEGDAVNVG
DVIMIIK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain

Homologues:

Organism=Homo sapiens, GI106049528, Length=621, Percent_Identity=34.7826086956522, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI106049295, Length=621, Percent_Identity=34.7826086956522, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI106049292, Length=621, Percent_Identity=34.7826086956522, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17562816, Length=612, Percent_Identity=35.7843137254902, Blast_Score=323, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6319695, Length=616, Percent_Identity=34.0909090909091, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6321376, Length=613, Percent_Identity=33.115823817292, Blast_Score=331, Evalue=2e-91,
Organism=Drosophila melanogaster, GI281363050, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652224, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652222, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652212, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652220, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652210, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652214, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI19921944, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652218, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24652216, Length=619, Percent_Identity=36.8336025848142, Blast_Score=334, Evalue=9e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYCB_METJA (Q58628)

Other databases:

- EMBL:   L77117
- PIR:   F64453
- RefSeq:   NP_248226.1
- ProteinModelPortal:   Q58628
- SMR:   Q58628
- GeneID:   1452127
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1231
- NMPDR:   fig|243232.1.peg.1263
- TIGR:   MJ1231
- HOGENOM:   HBG717218
- OMA:   ERYKSMT
- ProtClustDB:   PRK09282
- BioCyc:   MJAN243232:MJ_1231-MONOMER
- BRENDA:   6.4.1.1
- GO:   GO:0006094
- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR01108

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA; SSF51230 Hybrid_motif

EC number: =6.4.1.1

Molecular weight: Translated: 63908; Mature: 63908

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00188 BIOTIN; PS50968 BIOTINYL_LIPOYL; PS50991 PYR_CT

Important sites: BINDING 81-81 BINDING 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIVDTTFRDAQQSLIATRMRTEDMLPIAEKMDEVGFYSMEVWGGATFDACIRYLNEDP
CEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
WERLRALKKRIQNTPLQMLLRGQNLVGYRHYPDDIVEKFVIKAHENGIDIFRIFDALNDV
HHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RNMETAIKTAKKVGAEVQGAICYTISPVHTIDQYVELAKKLEEMGCDSICIKDMAGLLTP
HHHHHHHHHHHHHCCHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
YEGYELVKRLKEEISLPIDVHSHCTSGLAPMTYLKVIEAGADMVDCAISPFAMGTSQPPT
CCHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCC
ESIVVALKGTKYDTGLDLKLLNEIRDYFMKVREKYKMLFSPISQIVDARVLVYQVPGGML
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHH
SNLVSQLKEQGALDKFEEVLQEIPRVRKDLGYPPLVTPTSQIVGTQAVLNVLTEERYKII
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TNEVVNYVKGFYGKPPAPINPELLKRVLDEGEKPITCRPADLLPPEWEKVKKEAEEKGIV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCC
KKEEDILTYALYPQIAVKFLRGELKAEPIPKEKDIGKILEIPTEYIVEVDGEKFEVKIEP
CCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHEEEECCCEEEEEECC
KIGTELKRKKEVITAEMEGAVTSPFRGMVTKIKVKEGDKVKKGDVIVVLEAMKMEHPIES
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEHHHHCCCCCC
PVEGTVERILIDEGDAVNVGDVIMIIK
CHHHHHHHHEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MVKIVDTTFRDAQQSLIATRMRTEDMLPIAEKMDEVGFYSMEVWGGATFDACIRYLNEDP
CEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
WERLRALKKRIQNTPLQMLLRGQNLVGYRHYPDDIVEKFVIKAHENGIDIFRIFDALNDV
HHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RNMETAIKTAKKVGAEVQGAICYTISPVHTIDQYVELAKKLEEMGCDSICIKDMAGLLTP
HHHHHHHHHHHHHCCHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
YEGYELVKRLKEEISLPIDVHSHCTSGLAPMTYLKVIEAGADMVDCAISPFAMGTSQPPT
CCHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCC
ESIVVALKGTKYDTGLDLKLLNEIRDYFMKVREKYKMLFSPISQIVDARVLVYQVPGGML
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHH
SNLVSQLKEQGALDKFEEVLQEIPRVRKDLGYPPLVTPTSQIVGTQAVLNVLTEERYKII
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TNEVVNYVKGFYGKPPAPINPELLKRVLDEGEKPITCRPADLLPPEWEKVKKEAEEKGIV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCC
KKEEDILTYALYPQIAVKFLRGELKAEPIPKEKDIGKILEIPTEYIVEVDGEKFEVKIEP
CCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHEEEECCCEEEEEECC
KIGTELKRKKEVITAEMEGAVTSPFRGMVTKIKVKEGDKVKKGDVIVVLEAMKMEHPIES
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEHHHHCCCCCC
PVEGTVERILIDEGDAVNVGDVIMIIK
CHHHHHHHHEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096