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The map label for this gene is thrS [H]

Identifier: 15669383

GI number: 15669383

Start: 1140230

End: 1142104

Strand: Direct

Name: thrS [H]

Synonym: MJ1197

Alternate gene names: 15669383

Gene position: 1140230-1142104 (Clockwise)

Preceding gene: 15669380

Following gene: 15669384

Centisome position: 68.48

GC content: 34.56

Gene sequence:

>1875_bases
TTGAGGGATAATATGAAGATGCTACTAATCCATTCTGATTACTTAGAGTTTGAAGCTAAAGAAAAAACCAAGATTGCAGA
GGAAACAGAAAACTTAAAAGGTAAGTTGGATGAGTGTTTAGCCTGCTTTATTGCAGTTGAAAGAGAAGATGAAAACAATC
CAGAAGGAACTGCAATAGGAGCAGTTGAAGAGATTGAGAAAGTTGCTAATCAATTAAAGGTCAATAATATTGTTGTTTAT
CCTTATGCACATCTATCAAGTGATTTATCCTCACCAGAAACAGCAGTTAAGGTTTTAAAAGACATTGAGAGTATTTTAAA
AGAGAGAGGTTATAATGTCTTAAGAGCACCATTTGGATGGTATAAGGCATTTAAAATCAGCTGTAAAGGACATCCTTTGA
GTGAGTTGTCAAGAAAGATTGTAGCTAAGGAAGAAAAGAAAGAAGAAGGAGAAGAATCAAAATTCTACTTATTAAACCCA
GAAACAGAGGAAATTATTGAATTGAATGAAAACAACATAAATATAATTAAAGATGAAGAGTTGTTAGCTTTAGCTAAACA
TGAATTGGGAATTAGAGAGCATAAAGAACATGATGAACCTCCTCATGTAAAGTTTATTAAAGAAAAGGATATCTGCAGTT
ATGAGGAAGCATCAGACCCTGGACATTTCAGATGGTATCCAAAGGGCAAGTTGATGAGAGATTTGTTAGCTGATTATGTT
TATAACTTAGTTGTCAATATGGGAGCTATGCCAGTAGAAACACCAATTATGTATGATTTAGGAAACCCTGCTATTAGGGA
ACATGCTGATAAATTTGGAGAGAGGCAGTATAGGTTTAGACAAGGAAATAAAGAGCTAATGCTAAGATTTGCAGCATGCT
TTGGGCAGTTTATGATGAAAAAGGATATGTATCTATTGCCAAGATATTTGCCATTAAAACTCTATGAATTATCAACATAC
AGCTTTAGATATGAGCAGAGGGGGGAGTTGGTTGGTTTAAAGAGATTGAGATGCTTTACAATGCCTGATATGCATACTGT
CTGCTTAAACTTAGAGCAAGCAATGGAAGAGTTTGAAAAACAGTTCTGGGAATGTTTAAAAACTGGGGATGATTTAAATC
TAAGCTATTCAGTAATATTTAGATTCACAAAGGACTTCTTTGATGAGCATAGGGATTGGTTCTTTAAAATAGCAAAGGAA
TATAAGAACAAATATGGGAAAGATGTAATTTTAGAGATTTTACCAAAAAGAAAACACTATTGGGTTGGTAAGGTAGATAT
TGCTGTAATAGACAGCTTAGGAAGACCTATTGAGAACCCAACCGTGCAAATAGATGTTGAGAGTGCTAAGAGATTTGATA
TAAAAGTGCATACAAATGAAGGAGAGATTTATCCAATAATATTGCACTGCTCACCAACTGGTTCAATTGAGAGGGTTTTG
TGTGGTTTGTTGGAAAAAGCTGCTATAGAGGCTGAAAAAGGAAATGCTCCAATGTTGCCTGTTTGGCTCTCTCCGATACA
AGTTAGAGTTATTCCAGTGGCTGAAAGACATTATGACTATGCTTTAAAAGTAGCTGAGAAGTTGAGAGAAAACAACATTA
GGGCAGATTTTGATGATAGAGAAGAAAGTGTAAGCAAAAAGATTAGAAATGCAGGAAAAGAATGGGTTCCTTACGTTGTA
GTTATTGGAGATGAGGAAATGGAATCTGACAAATTGACAGTTACTATTAGAGAAAAATCAACCTTAAAGAAACCATATAA
GGAGAAGATGACATTAGATGAGTTGATTGAAAGGATTAAAAAAGAAACTGCTAACTACCCATATAGGCCTTTACCATTGC
CAATAAGATGCTCATTACAGCCAAAGTTCCATTAA

Upstream 100 bases:

>100_bases
TGTTAGATGCCCCATAACTACACCCTTATAAATTGATTAAACAAAGCAAAAATATTTAATATATCTTTTTCTTTTATAAT
TATCTTTATCTAAAATTTAA

Downstream 100 bases:

>100_bases
AAATTTTTTATAGCTATACTAAATATTCTTCTTATTGTTTTTATCACATGCATGAAAAACTATATAAATAACAATTGATA
TTTTATTTTTAAATAACTAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MRDNMKMLLIHSDYLEFEAKEKTKIAEETENLKGKLDECLACFIAVEREDENNPEGTAIGAVEEIEKVANQLKVNNIVVY
PYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGWYKAFKISCKGHPLSELSRKIVAKEEKKEEGEESKFYLLNP
ETEEIIELNENNINIIKDEELLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKLMRDLLADYV
YNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFAACFGQFMMKKDMYLLPRYLPLKLYELSTY
SFRYEQRGELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEKQFWECLKTGDDLNLSYSVIFRFTKDFFDEHRDWFFKIAKE
YKNKYGKDVILEILPKRKHYWVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEGEIYPIILHCSPTGSIERVL
CGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEKLRENNIRADFDDREESVSKKIRNAGKEWVPYVV
VIGDEEMESDKLTVTIREKSTLKKPYKEKMTLDELIERIKKETANYPYRPLPLPIRCSLQPKFH

Sequences:

>Translated_624_residues
MRDNMKMLLIHSDYLEFEAKEKTKIAEETENLKGKLDECLACFIAVEREDENNPEGTAIGAVEEIEKVANQLKVNNIVVY
PYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGWYKAFKISCKGHPLSELSRKIVAKEEKKEEGEESKFYLLNP
ETEEIIELNENNINIIKDEELLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKLMRDLLADYV
YNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFAACFGQFMMKKDMYLLPRYLPLKLYELSTY
SFRYEQRGELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEKQFWECLKTGDDLNLSYSVIFRFTKDFFDEHRDWFFKIAKE
YKNKYGKDVILEILPKRKHYWVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEGEIYPIILHCSPTGSIERVL
CGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEKLRENNIRADFDDREESVSKKIRNAGKEWVPYVV
VIGDEEMESDKLTVTIREKSTLKKPYKEKMTLDELIERIKKETANYPYRPLPLPIRCSLQPKFH
>Mature_624_residues
MRDNMKMLLIHSDYLEFEAKEKTKIAEETENLKGKLDECLACFIAVEREDENNPEGTAIGAVEEIEKVANQLKVNNIVVY
PYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGWYKAFKISCKGHPLSELSRKIVAKEEKKEEGEESKFYLLNP
ETEEIIELNENNINIIKDEELLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKLMRDLLADYV
YNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFAACFGQFMMKKDMYLLPRYLPLKLYELSTY
SFRYEQRGELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEKQFWECLKTGDDLNLSYSVIFRFTKDFFDEHRDWFFKIAKE
YKNKYGKDVILEILPKRKHYWVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEGEIYPIILHCSPTGSIERVL
CGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEKLRENNIRADFDDREESVSKKIRNAGKEWVPYVV
VIGDEEMESDKLTVTIREKSTLKKPYKEKMTLDELIERIKKETANYPYRPLPLPIRCSLQPKFH

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=413, Percent_Identity=27.6029055690073, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI38570111, Length=388, Percent_Identity=25.7731958762887, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI20070344, Length=407, Percent_Identity=26.044226044226, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1788013, Length=422, Percent_Identity=25.3554502369668, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71984192, Length=418, Percent_Identity=26.555023923445, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71984184, Length=418, Percent_Identity=26.555023923445, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322112, Length=414, Percent_Identity=27.2946859903382, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322654, Length=429, Percent_Identity=25.1748251748252, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24583839, Length=409, Percent_Identity=25.6723716381418, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24583842, Length=409, Percent_Identity=25.6723716381418, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI28574141, Length=409, Percent_Identity=25.6723716381418, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- ProDom:   PD016189 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72875; Mature: 72875

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDNMKMLLIHSDYLEFEAKEKTKIAEETENLKGKLDECLACFIAVEREDENNPEGTAIG
CCCCEEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEH
AVEEIEKVANQLKVNNIVVYPYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGW
HHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCC
YKAFKISCKGHPLSELSRKIVAKEEKKEEGEESKFYLLNPETEEIIELNENNINIIKDEE
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHEECCCCEEEEECHH
LLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKLMRDLLADYV
HHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
YNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFAACFGQFMMK
HHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KDMYLLPRYLPLKLYELSTYSFRYEQRGELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEK
CCHHHCCCCCCEEEEEEEEEEEEHHCCCCEECHHHHEEECCCCHHHHHHHHHHHHHHHHH
QFWECLKTGDDLNLSYSVIFRFTKDFFDEHRDWFFKIAKEYKNKYGKDVILEILPKRKHY
HHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCE
WVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEGEIYPIILHCSPTGSIERVL
EECEEHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHH
CGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEKLRENNIRADFDDR
HHHHHHHHHCCCCCCCCEEEEEECCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHH
EESVSKKIRNAGKEWVPYVVVIGDEEMESDKLTVTIREKSTLKKPYKEKMTLDELIERIK
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHCCCHHHCCCHHHHHHHHH
KETANYPYRPLPLPIRCSLQPKFH
HHHCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MRDNMKMLLIHSDYLEFEAKEKTKIAEETENLKGKLDECLACFIAVEREDENNPEGTAIG
CCCCEEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEH
AVEEIEKVANQLKVNNIVVYPYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGW
HHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCC
YKAFKISCKGHPLSELSRKIVAKEEKKEEGEESKFYLLNPETEEIIELNENNINIIKDEE
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHEECCCCEEEEECHH
LLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKLMRDLLADYV
HHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
YNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFAACFGQFMMK
HHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KDMYLLPRYLPLKLYELSTYSFRYEQRGELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEK
CCHHHCCCCCCEEEEEEEEEEEEHHCCCCEECHHHHEEECCCCHHHHHHHHHHHHHHHHH
QFWECLKTGDDLNLSYSVIFRFTKDFFDEHRDWFFKIAKEYKNKYGKDVILEILPKRKHY
HHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCE
WVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEGEIYPIILHCSPTGSIERVL
EECEEHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHH
CGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEKLRENNIRADFDDR
HHHHHHHHHCCCCCCCCEEEEEECCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHH
EESVSKKIRNAGKEWVPYVVVIGDEEMESDKLTVTIREKSTLKKPYKEKMTLDELIERIK
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHCCCHHHCCCHHHHHHHHH
KETANYPYRPLPLPIRCSLQPKFH
HHHCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]