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The map label for this gene is leuA2

Identifier: 15669381

GI number: 15669381

Start: 1137352

End: 1138908

Strand: Reverse

Name: leuA2

Synonym: MJ1195

Alternate gene names: 15669381

Gene position: 1138908-1137352 (Counterclockwise)

Preceding gene: 15669382

Following gene: 15669378

Centisome position: 68.4

GC content: 36.35

Gene sequence:

>1557_bases
ATGATAATTTATAGGGAAGAGAATGAAATTATAAAAAAGGCACTTGAGAATTTAAACATTCCAGATAGGGTTTATATCTT
TGACACAACACTCAGAGATGGAGAGCAAACTCCAGGTGTCTCTTTAACTCCAGAGGAGAAAATAGACATAGCCATAAAAT
TAGATGATTTAGGAGTTGATGTTATTGAGGCTGGTTTTCCAGTATCATCATTAGGAGAGCAGGAGGCTATTAAAAAAATC
TGCTCATTAAACTTAGATGCTGAAATCTGCGGATTGGCAAGGGCTGTAAAAAAGGATATAGATGTAGCTATAGATTGCGG
AGTTGATAGAATCCATACATTTATAGCAACCTCTCCATTGCATAGAAAATATAAATTAAAAAAATCAAAGGAAGAGATTA
TTGATATTGCAGTTGATGCCATAGAGTACATAAAAGAACATGGGATTAGAGTTGAGTTTTCAGCAGAAGATGCAACAAGA
ACAGAGATTGACTATTTAATAGAAGTTTATAAAAAGGCAGTAGATGCTGGAGCAGATATAATCAACGTTCCAGATACCGT
TGGAGTTATGATTCCAAGGGCTATGTATTATCTAATAAATGAGCTAAAGAAGGAAATAAAAGTCCCTATATCTGTGCATT
GCCACAACGACTTTGGTTTAGCTGTTGCAAACTCATTGGCAGCAGTTGAAGCAGGAGCAGAGCAAGTACATTGTACAATA
AACGGCTTAGGAGAGAGAGGAGGAAATGCAGCATTGGAAGAGGTAGTTATGAGCTTAATGTCAATCTATGGAGTTAAAAC
TAATATAAAAACACAAAAACTTTATGAGATATCTCAGCTTGTATCAAAATACACTGAAATTAAAGTCCAACCAAACAAGG
CAATTGTTGGAGAGAACGCTTTCGCTCATGAAAGTGGAATACATGCACATGGAGTTTTAGCTCATGCTTTAACCTATGAA
CCTATACCCCCAGAGTTAGTTGGGCAGAAGAGAAAAATAATCTTAGGTAAGCACACAGGAACACATGCAATTGAGGCAAA
GTTAAAAGAATTAGGAATTGAGGTTGGTAAGGATATAAATAAAGATCAATTTGATGAGATAGTTAAGAGAATTAAAGCTC
TTGGAGATAAAGGAAAGAGAGTCACTGACAGAGATGTTGAGGCAATAGTTGAGGATGTTGTTGGTAAGTTGGCTAAAAAA
GATAGAGTTGTTGAGTTGGAGCAAATAGCGGTTATGACAGGTAATAGAGTTATTCCAACTGCATCAGTTGCTTTAAAGAT
TGAAGAAGAGATTAAGAAGAGCTCAGCTATTGGTGTTGGACCAGTAGATGCGGCAGTTAAGGCAATACAAAAAGCCATTG
GAGAGAAGATTAAACTTAAAGAGTATCATATAAATGCCATAACTGGAGGAACTGATGCATTGGCGGAGGTTATTGTAACC
TTAGAAGGATATGGAAGGGAGATAACAACAAAGGCAGCAAGTGAAGATATAGTTAGGGCTTCAGTTGAGGCAGTTATAGA
TGGAATCAACAAAATCTTGGCAAAAAGAGAAAAATGA

Upstream 100 bases:

>100_bases
AAAGGTTAAGTTATAGCGTTTTAATGATATATCTGATTAGGTAAAATTTTTATAACATCCCATCATAAATAACAGAAAAT
TAAAAAGTTTTGGTGGGATT

Downstream 100 bases:

>100_bases
TTTCATCTCCTTATAAATTAATATAAAAATAAAATTATTTTTTATTTTATAAAATTTAATTTATGAATTCAAATAAACTA
ATTAAAATCTCCAAAATAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MIIYREENEIIKKALENLNIPDRVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVDVIEAGFPVSSLGEQEAIKKI
CSLNLDAEICGLARAVKKDIDVAIDCGVDRIHTFIATSPLHRKYKLKKSKEEIIDIAVDAIEYIKEHGIRVEFSAEDATR
TEIDYLIEVYKKAVDAGADIINVPDTVGVMIPRAMYYLINELKKEIKVPISVHCHNDFGLAVANSLAAVEAGAEQVHCTI
NGLGERGGNAALEEVVMSLMSIYGVKTNIKTQKLYEISQLVSKYTEIKVQPNKAIVGENAFAHESGIHAHGVLAHALTYE
PIPPELVGQKRKIILGKHTGTHAIEAKLKELGIEVGKDINKDQFDEIVKRIKALGDKGKRVTDRDVEAIVEDVVGKLAKK
DRVVELEQIAVMTGNRVIPTASVALKIEEEIKKSSAIGVGPVDAAVKAIQKAIGEKIKLKEYHINAITGGTDALAEVIVT
LEGYGREITTKAASEDIVRASVEAVIDGINKILAKREK

Sequences:

>Translated_518_residues
MIIYREENEIIKKALENLNIPDRVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVDVIEAGFPVSSLGEQEAIKKI
CSLNLDAEICGLARAVKKDIDVAIDCGVDRIHTFIATSPLHRKYKLKKSKEEIIDIAVDAIEYIKEHGIRVEFSAEDATR
TEIDYLIEVYKKAVDAGADIINVPDTVGVMIPRAMYYLINELKKEIKVPISVHCHNDFGLAVANSLAAVEAGAEQVHCTI
NGLGERGGNAALEEVVMSLMSIYGVKTNIKTQKLYEISQLVSKYTEIKVQPNKAIVGENAFAHESGIHAHGVLAHALTYE
PIPPELVGQKRKIILGKHTGTHAIEAKLKELGIEVGKDINKDQFDEIVKRIKALGDKGKRVTDRDVEAIVEDVVGKLAKK
DRVVELEQIAVMTGNRVIPTASVALKIEEEIKKSSAIGVGPVDAAVKAIQKAIGEKIKLKEYHINAITGGTDALAEVIVT
LEGYGREITTKAASEDIVRASVEAVIDGINKILAKREK
>Mature_518_residues
MIIYREENEIIKKALENLNIPDRVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVDVIEAGFPVSSLGEQEAIKKI
CSLNLDAEICGLARAVKKDIDVAIDCGVDRIHTFIATSPLHRKYKLKKSKEEIIDIAVDAIEYIKEHGIRVEFSAEDATR
TEIDYLIEVYKKAVDAGADIINVPDTVGVMIPRAMYYLINELKKEIKVPISVHCHNDFGLAVANSLAAVEAGAEQVHCTI
NGLGERGGNAALEEVVMSLMSIYGVKTNIKTQKLYEISQLVSKYTEIKVQPNKAIVGENAFAHESGIHAHGVLAHALTYE
PIPPELVGQKRKIILGKHTGTHAIEAKLKELGIEVGKDINKDQFDEIVKRIKALGDKGKRVTDRDVEAIVEDVVGKLAKK
DRVVELEQIAVMTGNRVIPTASVALKIEEEIKKSSAIGVGPVDAAVKAIQKAIGEKIKLKEYHINAITGGTDALAEVIVT
LEGYGREITTKAASEDIVRASVEAVIDGINKILAKREK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=305, Percent_Identity=27.5409836065574, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786261, Length=510, Percent_Identity=40.5882352941176, Blast_Score=344, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=36.3874345549738, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6320071, Length=376, Percent_Identity=36.1702127659575, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=24.5080500894454, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=571, Percent_Identity=24.8686514886165, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24582381, Length=305, Percent_Identity=24.9180327868852, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_METJA (Q58595)

Other databases:

- EMBL:   L77117
- PIR:   B64449
- RefSeq:   NP_248190.1
- ProteinModelPortal:   Q58595
- SMR:   Q58595
- GeneID:   1452090
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1195
- NMPDR:   fig|243232.1.peg.1227
- TIGR:   MJ1195
- HOGENOM:   HBG701974
- OMA:   CAHVTIN
- ProtClustDB:   PRK00915
- BioCyc:   MJAN243232:MJ_1195-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56621; Mature: 56621

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIYREENEIIKKALENLNIPDRVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVD
CEEEECHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCH
VIEAGFPVSSLGEQEAIKKICSLNLDAEICGLARAVKKDIDVAIDCGVDRIHTFIATSPL
HHHCCCCHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCH
HRKYKLKKSKEEIIDIAVDAIEYIKEHGIRVEFSAEDATRTEIDYLIEVYKKAVDAGADI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCE
INVPDTVGVMIPRAMYYLINELKKEIKVPISVHCHNDFGLAVANSLAAVEAGAEQVHCTI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
NGLGERGGNAALEEVVMSLMSIYGVKTNIKTQKLYEISQLVSKYTEIKVQPNKAIVGENA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCCCEEECCCC
FAHESGIHAHGVLAHALTYEPIPPELVGQKRKIILGKHTGTHAIEAKLKELGIEVGKDIN
CCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCC
KDQFDEIVKRIKALGDKGKRVTDRDVEAIVEDVVGKLAKKDRVVELEQIAVMTGNRVIPT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCEECCC
ASVALKIEEEIKKSSAIGVGPVDAAVKAIQKAIGEKIKLKEYHINAITGGTDALAEVIVT
HHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHH
LEGYGREITTKAASEDIVRASVEAVIDGINKILAKREK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIIYREENEIIKKALENLNIPDRVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVD
CEEEECHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCH
VIEAGFPVSSLGEQEAIKKICSLNLDAEICGLARAVKKDIDVAIDCGVDRIHTFIATSPL
HHHCCCCHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCH
HRKYKLKKSKEEIIDIAVDAIEYIKEHGIRVEFSAEDATRTEIDYLIEVYKKAVDAGADI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCE
INVPDTVGVMIPRAMYYLINELKKEIKVPISVHCHNDFGLAVANSLAAVEAGAEQVHCTI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
NGLGERGGNAALEEVVMSLMSIYGVKTNIKTQKLYEISQLVSKYTEIKVQPNKAIVGENA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCCCEEECCCC
FAHESGIHAHGVLAHALTYEPIPPELVGQKRKIILGKHTGTHAIEAKLKELGIEVGKDIN
CCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCC
KDQFDEIVKRIKALGDKGKRVTDRDVEAIVEDVVGKLAKKDRVVELEQIAVMTGNRVIPT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCEECCC
ASVALKIEEEIKKSSAIGVGPVDAAVKAIQKAIGEKIKLKEYHINAITGGTDALAEVIVT
HHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHH
LEGYGREITTKAASEDIVRASVEAVIDGINKILAKREK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087