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The map label for this gene is gatA

Identifier: 15669349

GI number: 15669349

Start: 1100667

End: 1101971

Strand: Direct

Name: gatA

Synonym: MJ1160

Alternate gene names: 15669349

Gene position: 1100667-1101971 (Clockwise)

Preceding gene: 15669348

Following gene: 15669350

Centisome position: 66.11

GC content: 34.79

Gene sequence:

>1305_bases
ATGATTGTTGAGAGAGTTGAAGAGTATTTGGACAGAATAGAAAAAATCAACAAGGATATTAATGCTCTAATTGAGGTAAA
ACCAGAAAAAGTTTTAGAAGAGGCAAAAAAATTAGAAAAAGATGAAAAAGCTAAGAAAAAGCCATTATATGGAAAGATTA
TTGTAGTTAAAGCAAACATAAACGTTGAGGGCTATACAATCTCATGTGCATCAAAGACTTTAGAAAACTACATAGCCCCT
TACGATGCCACTGTTATAGAGAAGATTAAAGAAAATGGTGGATTGATAATAGGAATAGCAAATATGGATGAGTTTGCATG
TGGTAGTAGTGGAGAAACCTCTTATTTTGGACCAACAAAAAACCCAAGGGCTAAAGATAGAATTCCTGGAGGAAGTTCTT
CAGGAAGTGCTGCTGCAGTATCTGCAGATTTATGTGATATGGCTTTAGGTAGTGACACAGGGGGAAGTATTAGGAACCCT
GCTTCACATTGCGGAGTTGTTGGATTTAAGCCAAGTTATGGAGTTGTTAGCAGATATGGCCTCTGTGATTTGGCAATGAG
TTTTGACCAAATAGGACCTTTAACAAAAACAGCTGAAGATGCATTATTATTAACAAATATCATTAAAGGTAAAGATTTAA
GGGACACAACAACGGTAGAGACAAAACCTTTTGAAAAGAAAGATATTAAAGGCTTTAAGGTTGGAGTTGTTAAGGAGTTT
ATGGATGTTGCCGATGAGAAGATAAGGGATAAGGTAGAAAAAGCCATTGAAGTCTTTAAAGATTTAGGTTGTGAGATTGT
TGAATTAAGCTATAAATATGTTGATTTAGCATTGCCAACTTACTATTTAATCAACTACGTTGAGTTCTTCTCATCCACAA
GAAGATACGATGGAAGAAGATATGGATATAAAATAGAGGAAGTTTGTGGAGAGGAAGTTTTAAGAAGAATTATGATTGGT
TCAATGATTAGTCAGAAAGAGTATAGTGGTAAATACTACAAAAACGCTTTAAAGGCAAGGAATTTAATGAGAAATGAGAT
GATTAAGATTATGAAGGATGTTGATATTATAGTGGGAGCAACAGTTCCTAAGTTACCACACAAATTAGGTGAAAAATTAA
CACCAATGGAGATGTATAGTTATGATGTTTTAACAGTCCCAGCTAATATCTGCGGTTTGTGTGCTGGAGTTGTTCCATGT
GGGGATATAAATGGAATTCCTGTTGGTTTGCAAATCCAAGGAAAGCCATTTGAAGATGAAAAAGTTCTAAGTGCAATGAT
TGCATTTGAGAAGGCAATGGAGTAA

Upstream 100 bases:

>100_bases
TAGATGTTGAGAAAGAAATAAGAATATTGGAGGAAATGGAATAACAAAAAATAAATAAATATAAGTCAATATTATAACCA
AAAAAATTTACGGTGATGAG

Downstream 100 bases:

>100_bases
TATTTCCATATTAATACTATTTTTAATTTTTTGATGAATTTTTATTATTTTAATATATTAAATTAAGTTCTAAATTCAAA
TGAAAACATTTTTAAATGAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIVERVEEYLDRIEKINKDINALIEVKPEKVLEEAKKLEKDEKAKKKPLYGKIIVVKANINVEGYTISCASKTLENYIAP
YDATVIEKIKENGGLIIGIANMDEFACGSSGETSYFGPTKNPRAKDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNP
ASHCGVVGFKPSYGVVSRYGLCDLAMSFDQIGPLTKTAEDALLLTNIIKGKDLRDTTTVETKPFEKKDIKGFKVGVVKEF
MDVADEKIRDKVEKAIEVFKDLGCEIVELSYKYVDLALPTYYLINYVEFFSSTRRYDGRRYGYKIEEVCGEEVLRRIMIG
SMISQKEYSGKYYKNALKARNLMRNEMIKIMKDVDIIVGATVPKLPHKLGEKLTPMEMYSYDVLTVPANICGLCAGVVPC
GDINGIPVGLQIQGKPFEDEKVLSAMIAFEKAME

Sequences:

>Translated_434_residues
MIVERVEEYLDRIEKINKDINALIEVKPEKVLEEAKKLEKDEKAKKKPLYGKIIVVKANINVEGYTISCASKTLENYIAP
YDATVIEKIKENGGLIIGIANMDEFACGSSGETSYFGPTKNPRAKDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNP
ASHCGVVGFKPSYGVVSRYGLCDLAMSFDQIGPLTKTAEDALLLTNIIKGKDLRDTTTVETKPFEKKDIKGFKVGVVKEF
MDVADEKIRDKVEKAIEVFKDLGCEIVELSYKYVDLALPTYYLINYVEFFSSTRRYDGRRYGYKIEEVCGEEVLRRIMIG
SMISQKEYSGKYYKNALKARNLMRNEMIKIMKDVDIIVGATVPKLPHKLGEKLTPMEMYSYDVLTVPANICGLCAGVVPC
GDINGIPVGLQIQGKPFEDEKVLSAMIAFEKAME
>Mature_434_residues
MIVERVEEYLDRIEKINKDINALIEVKPEKVLEEAKKLEKDEKAKKKPLYGKIIVVKANINVEGYTISCASKTLENYIAP
YDATVIEKIKENGGLIIGIANMDEFACGSSGETSYFGPTKNPRAKDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNP
ASHCGVVGFKPSYGVVSRYGLCDLAMSFDQIGPLTKTAEDALLLTNIIKGKDLRDTTTVETKPFEKKDIKGFKVGVVKEF
MDVADEKIRDKVEKAIEVFKDLGCEIVELSYKYVDLALPTYYLINYVEFFSSTRRYDGRRYGYKIEEVCGEEVLRRIMIG
SMISQKEYSGKYYKNALKARNLMRNEMIKIMKDVDIIVGATVPKLPHKLGEKLTPMEMYSYDVLTVPANICGLCAGVVPC
GDINGIPVGLQIQGKPFEDEKVLSAMIAFEKAME

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=487, Percent_Identity=32.8542094455852, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI195972892, Length=475, Percent_Identity=25.0526315789474, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=414, Percent_Identity=35.2657004830918, Blast_Score=207, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17537465, Length=471, Percent_Identity=25.0530785562633, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=290, Percent_Identity=27.2413793103448, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=252, Percent_Identity=25.3968253968254, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=284, Percent_Identity=24.6478873239437, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=284, Percent_Identity=24.6478873239437, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538252, Length=176, Percent_Identity=28.4090909090909, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538254, Length=160, Percent_Identity=31.875, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=465, Percent_Identity=32.9032258064516, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319685, Length=411, Percent_Identity=26.5206812652068, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=31.6593886462882, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24648435, Length=465, Percent_Identity=25.5913978494624, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=465, Percent_Identity=25.5913978494624, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550774, Length=465, Percent_Identity=25.5913978494624, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24644968, Length=466, Percent_Identity=27.2532188841202, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161078093, Length=473, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652985, Length=475, Percent_Identity=24.4210526315789, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922090, Length=475, Percent_Identity=24.4210526315789, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652981, Length=475, Percent_Identity=24.4210526315789, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652983, Length=475, Percent_Identity=24.4210526315789, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356731, Length=454, Percent_Identity=25.1101321585903, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=414, Percent_Identity=25.8454106280193, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648439, Length=414, Percent_Identity=25.8454106280193, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_METJA (Q58560)

Other databases:

- EMBL:   L77117
- PIR:   G64444
- RefSeq:   NP_248154.1
- ProteinModelPortal:   Q58560
- SMR:   Q58560
- GeneID:   1452058
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1160
- NMPDR:   fig|243232.1.peg.1191
- TIGR:   MJ1160
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   MJAN243232:MJ_1160-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 48008; Mature: 48008

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 57-57 ACT_SITE 132-132 ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVERVEEYLDRIEKINKDINALIEVKPEKVLEEAKKLEKDEKAKKKPLYGKIIVVKANI
CCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
NVEGYTISCASKTLENYIAPYDATVIEKIKENGGLIIGIANMDEFACGSSGETSYFGPTK
CCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCC
NPRAKDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPASHCGVVGFKPSYGVVSRYG
CCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHCCCEECCCCCCHHHHCC
LCDLAMSFDQIGPLTKTAEDALLLTNIIKGKDLRDTTTVETKPFEKKDIKGFKVGVVKEF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
MDVADEKIRDKVEKAIEVFKDLGCEIVELSYKYVDLALPTYYLINYVEFFSSTRRYDGRR
HHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YGYKIEEVCGEEVLRRIMIGSMISQKEYSGKYYKNALKARNLMRNEMIKIMKDVDIIVGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TVPKLPHKLGEKLTPMEMYSYDVLTVPANICGLCAGVVPCGDINGIPVGLQIQGKPFEDE
CCCHHHHHHCCCCCCHHHHCCEEEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
KVLSAMIAFEKAME
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIVERVEEYLDRIEKINKDINALIEVKPEKVLEEAKKLEKDEKAKKKPLYGKIIVVKANI
CCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
NVEGYTISCASKTLENYIAPYDATVIEKIKENGGLIIGIANMDEFACGSSGETSYFGPTK
CCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCC
NPRAKDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPASHCGVVGFKPSYGVVSRYG
CCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHCCCEECCCCCCHHHHCC
LCDLAMSFDQIGPLTKTAEDALLLTNIIKGKDLRDTTTVETKPFEKKDIKGFKVGVVKEF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
MDVADEKIRDKVEKAIEVFKDLGCEIVELSYKYVDLALPTYYLINYVEFFSSTRRYDGRR
HHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YGYKIEEVCGEEVLRRIMIGSMISQKEYSGKYYKNALKARNLMRNEMIKIMKDVDIIVGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TVPKLPHKLGEKLTPMEMYSYDVLTVPANICGLCAGVVPCGDINGIPVGLQIQGKPFEDE
CCCHHHHHHCCCCCCHHHHCCEEEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
KVLSAMIAFEKAME
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087