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The map label for this gene is asnB [C]

Identifier: 15669245

GI number: 15669245

Start: 994642

End: 996192

Strand: Direct

Name: asnB [C]

Synonym: MJ1056

Alternate gene names: 15669245

Gene position: 994642-996192 (Clockwise)

Preceding gene: 15669244

Following gene: 15669246

Centisome position: 59.74

GC content: 30.11

Gene sequence:

>1551_bases
ATGATGTGTGGGATAAATGGGATTATTAGATTTGGTAAAGAAGTTATAAAAGAAGAAATTAATAAGATGAATAAAGCAAT
TAAACATAGAGGTCCTGATGATGAAGGAATCTTTATTTATAATTTTAAAAATTATTCTATTGGTTTGGGACATGTTAGAT
TAGCAATTTTAGATTTAAGTGAAAAGGGACATCAACCGATGGGGTATAATGTTGATGAGGATAAAATCATTTATAGAGAT
GATGAGTTGGATAGGGCGGATATAATCATTGTTTATAATGGAGAGATTTATAATTATTTGGAGTTAAAAGAAAAATTTAA
CTTAGAGACAGAAACAGGGACAGATACAGAGGTTATTTTAAAGCTTTATAATAAGTTGGGTTTTGATTGTGTTAAAGAGT
TTAATGGTATGTGGGCATTTTGTATTTTTGATAAAAAGAAGGGCTTAATCTTTTGTTCAAGGGATAGATTAGGAGTTAAG
CCATTTTATTATTATTGGGATGGAAATGAATTTATCTTTTCTTCTGAATTGAAGGGAATTTTAGCAGTTAAAGAGATAAA
TAAAAAAGAAAATATTAACAAAGATGCAGTTGAGTTATACTTTGCCTTGGGATTTATCCCATCTCCCTACTCTATTTATA
AAAACACTTTTAAATTAGAGGCAAGACAGAATTTAATCTTTGATTTGGATAAAAGAGAGATTAGAAAATATTATTACTGG
GAGTTGCCAGATTACAAACCTATTTATGATAAAAAGAAATTGATTGAAGAAGGTAAAAAGCTATTATATGATGCTGTTAA
GATAAGAATGAGGAGTGATGTGCCAGTTGGAGCGTTTTTGAGTGGTGGTTTAGATAGCTCTACAGTTGTTGGAGTTATGA
GGGAATTTACAGATTTAAGTAAATTGCATACTTTTTCTATAGGTTTTGAAGGAAAGTATGATGAAACTCCTTATATTAAG
ATAGTTGTTGATTACTTTAAAACTCAGCATCATCATTACTACTTTAAGGAGAGAGATTTTGAGGAATTGATTGATAAATA
CAGCTGGATTTACGATGAACCGTTTGGAGATTATAGTGGATTCCCTACTTATAAGGTCTCTGAAATGGCAAGGAAATTTG
TTACTGTTGTTTTGAGTGGAGATGGCGGGGATGAGGTTTTTGGAGGTTATATGACTCATTTAAACGGATATAGAATGGAT
TTCATTAGAAAATTACCTAAATTTTTGAGAGTTGTTGGTTCTAAATTACCTGTTAAGAAAGATTTAAATGGAATTGCTAA
TTTGTATTTATTAAAGGAGGCGTTTAGGTTATCCTTAATAAATCCAGAGGAATTCTATGCTGAATCAATAAAAGAAGATG
CGATAAGACCAGAAATTTATAAAAAATGGACTATTGAAAAATTAAGATATTGTTTGAATAAAGGAGATAATAAGTTGGGG
GAAGCCCTAAGGATTTTTGATTTATTGTTTAATACTTTGTGTGATAATTTCTTAGTTAAGGTTGATAGGGCATCTATGCT
AACGCTTTGGAAGTTAGAAGTCCATTTTTAG

Upstream 100 bases:

>100_bases
TATAAACCAAAAGTTACTATTGAGGAGGGTTTAAAGAGATTTTGTAACTGGTTTTTGGAAAATAAAGATTGGCTTTTAAG
ATTATAATTTTTTTGGTGGA

Downstream 100 bases:

>100_bases
ATTATAGGTTTGCAGAATTTAGCCAAAAAATTCCAACTGAATGGAAGGTAGATTTGTTCAAAACCAAGAAGTTGATGAGG
GAGATTATTAAAGATATTTT

Product: asparagine synthetase AsnB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MMCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLSEKGHQPMGYNVDEDKIIYRD
DELDRADIIIVYNGEIYNYLELKEKFNLETETGTDTEVILKLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVK
PFYYYWDGNEFIFSSELKGILAVKEINKKENINKDAVELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIRKYYYW
ELPDYKPIYDKKKLIEEGKKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGVMREFTDLSKLHTFSIGFEGKYDETPYIK
IVVDYFKTQHHHYYFKERDFEELIDKYSWIYDEPFGDYSGFPTYKVSEMARKFVTVVLSGDGGDEVFGGYMTHLNGYRMD
FIRKLPKFLRVVGSKLPVKKDLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYKKWTIEKLRYCLNKGDNKLG
EALRIFDLLFNTLCDNFLVKVDRASMLTLWKLEVHF

Sequences:

>Translated_516_residues
MMCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLSEKGHQPMGYNVDEDKIIYRD
DELDRADIIIVYNGEIYNYLELKEKFNLETETGTDTEVILKLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVK
PFYYYWDGNEFIFSSELKGILAVKEINKKENINKDAVELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIRKYYYW
ELPDYKPIYDKKKLIEEGKKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGVMREFTDLSKLHTFSIGFEGKYDETPYIK
IVVDYFKTQHHHYYFKERDFEELIDKYSWIYDEPFGDYSGFPTYKVSEMARKFVTVVLSGDGGDEVFGGYMTHLNGYRMD
FIRKLPKFLRVVGSKLPVKKDLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYKKWTIEKLRYCLNKGDNKLG
EALRIFDLLFNTLCDNFLVKVDRASMLTLWKLEVHF
>Mature_516_residues
MMCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLSEKGHQPMGYNVDEDKIIYRD
DELDRADIIIVYNGEIYNYLELKEKFNLETETGTDTEVILKLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVK
PFYYYWDGNEFIFSSELKGILAVKEINKKENINKDAVELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIRKYYYW
ELPDYKPIYDKKKLIEEGKKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGVMREFTDLSKLHTFSIGFEGKYDETPYIK
IVVDYFKTQHHHYYFKERDFEELIDKYSWIYDEPFGDYSGFPTYKVSEMARKFVTVVLSGDGGDEVFGGYMTHLNGYRMD
FIRKLPKFLRVVGSKLPVKKDLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYKKWTIEKLRYCLNKGDNKLG
EALRIFDLLFNTLCDNFLVKVDRASMLTLWKLEVHF

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=411, Percent_Identity=27.2506082725061, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI168229252, Length=411, Percent_Identity=27.2506082725061, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI168229250, Length=411, Percent_Identity=27.2506082725061, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI296010848, Length=386, Percent_Identity=27.720207253886, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI296010852, Length=310, Percent_Identity=26.4516129032258, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI296010850, Length=310, Percent_Identity=26.4516129032258, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1786889, Length=412, Percent_Identity=28.1553398058252, Blast_Score=145, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25147557, Length=412, Percent_Identity=31.3106796116505, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993933, Length=412, Percent_Identity=31.3106796116505, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=373, Percent_Identity=29.4906166219839, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560178, Length=403, Percent_Identity=27.0471464019851, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321563, Length=432, Percent_Identity=28.0092592592593, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=431, Percent_Identity=26.9141531322506, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45553209, Length=437, Percent_Identity=27.4599542334096, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 60605; Mature: 60605

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECCEEEEEEEECC
EKGHQPMGYNVDEDKIIYRDDELDRADIIIVYNGEIYNYLELKEKFNLETETGTDTEVIL
CCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCEEEHHHHHHHCCCCCCCCCCHHHHH
KLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVKPFYYYWDGNEFIFSSELKGI
HHHHHHCHHHHHHHCCEEEEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCC
LAVKEINKKENINKDAVELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIRKYYYW
HHHHHCCHHCCCCHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCCEEECCHHHHCCEEEE
ELPDYKPIYDKKKLIEEGKKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGVMREFTDLS
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHH
KLHTFSIGFEGKYDETPYIKIVVDYFKTQHHHYYFKERDFEELIDKYSWIYDEPFGDYSG
HHEEEEECCCCCCCCCCEEEEEEEHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCC
FPTYKVSEMARKFVTVVLSGDGGDEVFGGYMTHLNGYRMDFIRKLPKFLRVVGSKLPVKK
CCCCHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHH
DLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYKKWTIEKLRYCLNKGDNKLG
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHH
EALRIFDLLFNTLCDNFLVKVDRASMLTLWKLEVHF
HHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEC
>Mature Secondary Structure
MMCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECCEEEEEEEECC
EKGHQPMGYNVDEDKIIYRDDELDRADIIIVYNGEIYNYLELKEKFNLETETGTDTEVIL
CCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCEEEHHHHHHHCCCCCCCCCCHHHHH
KLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVKPFYYYWDGNEFIFSSELKGI
HHHHHHCHHHHHHHCCEEEEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCC
LAVKEINKKENINKDAVELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIRKYYYW
HHHHHCCHHCCCCHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCCEEECCHHHHCCEEEE
ELPDYKPIYDKKKLIEEGKKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGVMREFTDLS
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHH
KLHTFSIGFEGKYDETPYIKIVVDYFKTQHHHYYFKERDFEELIDKYSWIYDEPFGDYSG
HHEEEEECCCCCCCCCCEEEEEEEHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCC
FPTYKVSEMARKFVTVVLSGDGGDEVFGGYMTHLNGYRMDFIRKLPKFLRVVGSKLPVKK
CCCCHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHH
DLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYKKWTIEKLRYCLNKGDNKLG
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHH
EALRIFDLLFNTLCDNFLVKVDRASMLTLWKLEVHF
HHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]