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The map label for this gene is carA

Identifier: 15669208

GI number: 15669208

Start: 949258

End: 950322

Strand: Direct

Name: carA

Synonym: MJ1019

Alternate gene names: 15669208

Gene position: 949258-950322 (Clockwise)

Preceding gene: 15669206

Following gene: 15669209

Centisome position: 57.01

GC content: 33.05

Gene sequence:

>1065_bases
ATGGAGGCAGTGTTAATCTTAGAGGACGGAACAATTTTAAAAGGAAAAGGTTTTGGAGCAGAGAAAGAGGTTTTTGGAGA
ATTAGTTTTTACAACAGTTATGACTGGTTATGTTGAAGTTTTAACAGACCCTTCATATAAAGGGCAAATAGTTATGATGA
CTTATCCATTAGAAGGGAATTATGGAGTTAAAAAGGATTGGTTTGAGTCAGATGGGATAAAGGCAGAGGGTTTTGTTGTT
AGAGAGGTAACAAGTAAAGCTTTAGATGACTTTTTAAAGGAGTATGATATCCCAGGAATTCAAGATATTGATACAAGATT
CTTAACAAGAAAGATTAGGGATAAAGGAGTTGTTAAAAGCTGTTTAAAGGTTGCTGAAGAAATAAGTGATGATGAAATAT
CTGAATTATTGGAGAGAGTTAAAAGATACAGTGATATATCAGATATTGATTTAGTTCCTTTAGTTTCAACAAAAGAACCA
AAAATTCATAAAACAGCTAACCCAAAGGCAAGATGTGTTTTAATTGATTGTGGAGTTAAATTGAATATTATAAGAAGTTT
AGTTCAAAGAAACTGTGAAGTTATTCAAGTCCCATACAACACAAAGTATGATGAAATCTTAGAATACAAACCAGATTTTG
TTTTAATCTCCAATGGCCCAGGAGACCCAGCAAGATTAAAAGAAGTTATTAAAAATATTAAAAACTTAATTGGTGTTGTC
CCAATAACAGGAATTTGTTTAGGTAATCAACTTTTATCCTTAGCATTTGGTGGAGAAACATACAAGATGAAGTTTGGGCA
TAGGGGAGGAAACCAGCCAGTTAAAGATTTAAAAACACAAAAAGTTTATATAACCTCCCAAAACCATGGATTTGCTGTTA
GAAAGGAGAGCTTACCAGATGATGTAGAAGTTAGCTTTATAAATCTAAACGATATGACTGTTGAAGGTATTAGGCATAAG
GATTTACCAATATTCTCAGTTCAATTCCACCCAGAGGCAAGACCAGGACCTCATGACACAATGTTTTTATTCGATGAGAT
GATTAAGCTGAAGGATAGGAAATAA

Upstream 100 bases:

>100_bases
CATAAATTATAAAATGCATAATGTCCTCTAAAAGCAGTAGATAGATGATTATCCCAATCATATATATATGTGATAGTTTA
TAGGTATTTTGGTGAAATAT

Downstream 100 bases:

>100_bases
ACTTCTAAAAAATATTTTTATTTTATTGCCTTGAAGGTTAATTTAATTTTAAATTTAAATTATTTTAATTAAATTAAAAA
CAAGTTTTATCTAAATGGGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEAVLILEDGTILKGKGFGAEKEVFGELVFTTVMTGYVEVLTDPSYKGQIVMMTYPLEGNYGVKKDWFESDGIKAEGFVV
REVTSKALDDFLKEYDIPGIQDIDTRFLTRKIRDKGVVKSCLKVAEEISDDEISELLERVKRYSDISDIDLVPLVSTKEP
KIHKTANPKARCVLIDCGVKLNIIRSLVQRNCEVIQVPYNTKYDEILEYKPDFVLISNGPGDPARLKEVIKNIKNLIGVV
PITGICLGNQLLSLAFGGETYKMKFGHRGGNQPVKDLKTQKVYITSQNHGFAVRKESLPDDVEVSFINLNDMTVEGIRHK
DLPIFSVQFHPEARPGPHDTMFLFDEMIKLKDRK

Sequences:

>Translated_354_residues
MEAVLILEDGTILKGKGFGAEKEVFGELVFTTVMTGYVEVLTDPSYKGQIVMMTYPLEGNYGVKKDWFESDGIKAEGFVV
REVTSKALDDFLKEYDIPGIQDIDTRFLTRKIRDKGVVKSCLKVAEEISDDEISELLERVKRYSDISDIDLVPLVSTKEP
KIHKTANPKARCVLIDCGVKLNIIRSLVQRNCEVIQVPYNTKYDEILEYKPDFVLISNGPGDPARLKEVIKNIKNLIGVV
PITGICLGNQLLSLAFGGETYKMKFGHRGGNQPVKDLKTQKVYITSQNHGFAVRKESLPDDVEVSFINLNDMTVEGIRHK
DLPIFSVQFHPEARPGPHDTMFLFDEMIKLKDRK
>Mature_354_residues
MEAVLILEDGTILKGKGFGAEKEVFGELVFTTVMTGYVEVLTDPSYKGQIVMMTYPLEGNYGVKKDWFESDGIKAEGFVV
REVTSKALDDFLKEYDIPGIQDIDTRFLTRKIRDKGVVKSCLKVAEEISDDEISELLERVKRYSDISDIDLVPLVSTKEP
KIHKTANPKARCVLIDCGVKLNIIRSLVQRNCEVIQVPYNTKYDEILEYKPDFVLISNGPGDPARLKEVIKNIKNLIGVV
PITGICLGNQLLSLAFGGETYKMKFGHRGGNQPVKDLKTQKVYITSQNHGFAVRKESLPDDVEVSFINLNDMTVEGIRHK
DLPIFSVQFHPEARPGPHDTMFLFDEMIKLKDRK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=368, Percent_Identity=43.2065217391304, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI169790915, Length=369, Percent_Identity=39.5663956639566, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI21361331, Length=369, Percent_Identity=39.5663956639566, Blast_Score=259, Evalue=2e-69,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=42.627345844504, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI193204318, Length=367, Percent_Identity=41.9618528610354, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6322331, Length=385, Percent_Identity=41.8181818181818, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324878, Length=374, Percent_Identity=40.3743315508021, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6322638, Length=191, Percent_Identity=31.413612565445, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45555749, Length=384, Percent_Identity=41.1458333333333, Blast_Score=264, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24642586, Length=384, Percent_Identity=41.1458333333333, Blast_Score=264, Evalue=6e-71,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_METJA (Q58425)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_248013.1
- ProteinModelPortal:   Q58425
- SMR:   Q58425
- GeneID:   1451916
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1019
- NMPDR:   fig|243232.1.peg.1050
- TIGR:   MJ1019
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   MJAN243232:MJ_1019-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_A
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39921; Mature: 39921

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 246-246 ACT_SITE 330-330 ACT_SITE 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVLILEDGTILKGKGFGAEKEVFGELVFTTVMTGYVEVLTDPSYKGQIVMMTYPLEGN
CCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCC
YGVKKDWFESDGIKAEGFVVREVTSKALDDFLKEYDIPGIQDIDTRFLTRKIRDKGVVKS
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
CLKVAEEISDDEISELLERVKRYSDISDIDLVPLVSTKEPKIHKTANPKARCVLIDCGVK
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCEEEEEEECCCC
LNIIRSLVQRNCEVIQVPYNTKYDEILEYKPDFVLISNGPGDPARLKEVIKNIKNLIGVV
HHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
PITGICLGNQLLSLAFGGETYKMKFGHRGGNQPVKDLKTQKVYITSQNHGFAVRKESLPD
HHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCHHHCCCEEEEEEECCCCEEEECCCCCC
DVEVSFINLNDMTVEGIRHKDLPIFSVQFHPEARPGPHDTMFLFDEMIKLKDRK
CCEEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEAVLILEDGTILKGKGFGAEKEVFGELVFTTVMTGYVEVLTDPSYKGQIVMMTYPLEGN
CCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCC
YGVKKDWFESDGIKAEGFVVREVTSKALDDFLKEYDIPGIQDIDTRFLTRKIRDKGVVKS
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
CLKVAEEISDDEISELLERVKRYSDISDIDLVPLVSTKEPKIHKTANPKARCVLIDCGVK
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCEEEEEEECCCC
LNIIRSLVQRNCEVIQVPYNTKYDEILEYKPDFVLISNGPGDPARLKEVIKNIKNLIGVV
HHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
PITGICLGNQLLSLAFGGETYKMKFGHRGGNQPVKDLKTQKVYITSQNHGFAVRKESLPD
HHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCHHHCCCEEEEEEECCCCEEEECCCCCC
DVEVSFINLNDMTVEGIRHKDLPIFSVQFHPEARPGPHDTMFLFDEMIKLKDRK
CCEEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087