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The map label for this gene is cofF [H]

Identifier: 15669190

GI number: 15669190

Start: 930004

End: 930876

Strand: Direct

Name: cofF [H]

Synonym: MJ1001

Alternate gene names: 15669190

Gene position: 930004-930876 (Clockwise)

Preceding gene: 15669189

Following gene: 15669191

Centisome position: 55.86

GC content: 30.93

Gene sequence:

>873_bases
ATGTTTATGGTAAAAATAACCATACTATCACCAGAAGGAAGGAGCTGTAGTGTATGGAGTTTAAAGAATGAGATTGAAAA
ATTAGGAGCTAAATGCGATATATTTTTATTATCAAGCCCAGAAAATTTGATGAGCCATGATTTTAAATTAGAAACTGACT
TAATTCATTCGAGATGTGGAATAGGAGATTATTTTGATAGATTAACCCTCTACTCTTGGCAATTCATAAATGCATTGGAA
GTTGAGGGCTGTAGATTTATAAATCCAATTAAAACTCTCTACCTTACATCAGACAAGTTTAAATGTATAAAGTTACTTGC
AAAGAATAAAATAAAGACACCAAAAACAGCTTTAATTAGAGATTATGAGGATGCAGTTAAATTTATTGAGAAATACAATT
TAAGATTTCCAGTGGTTATAAAAAATTCTTTCTCAAAGTGTGGTTTAAAGGTATTTATGGCAAGAAATTATGATGAGTTA
AAACAATTAACAAAAAATGCCATCTGGGAAGGAAAGCTTATACAGGAGTTTATTGATTTCAAAGAAAATGACTTATATAG
GGATATGAGAATATTAGTTGTTGATGGAGAAGTTGTTGGTGGATACAGAAGAGTTAGTAGAGATTTTAGAACAAACCTTT
ATTTAGGAAATGTTGTTGAGAAACTAAATATTGATGAGGAACTTGAAGAATTAGCCTTAAAATGTGCTGATTTATCTGAG
GCTGTAATCTTAGGGGTTGATATACTACCAACAAAAGACAACTATTATGTTATTGAGTTAAATTCATCCCCGGGAACTAA
GGGCTTTAGAGATATAGGAATAAATGCTGATAAGAAGATAGCTGAAGCTTTAGTTAGGTATGCAAAGTCCTGA

Upstream 100 bases:

>100_bases
TGTAAAAGATATGATAACTGGAGAGCAGAAATTAATTGGTATAGATGAATTGACAAATTTCTAACCCCAATTTAAATCTT
TATAAATTTTTATTTTTGGG

Downstream 100 bases:

>100_bases
AGAGACATTAAGGGGATGAAAGCTCCTTTTAATATATCCGTTTTGGTTATACCGAATTGAATTATGTAAAGTTGTAGTGG
TGGAGGTATGATAGCTAAAT

Product: ribosomal protein S6 modification protein 2 RimK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MFMVKITILSPEGRSCSVWSLKNEIEKLGAKCDIFLLSSPENLMSHDFKLETDLIHSRCGIGDYFDRLTLYSWQFINALE
VEGCRFINPIKTLYLTSDKFKCIKLLAKNKIKTPKTALIRDYEDAVKFIEKYNLRFPVVIKNSFSKCGLKVFMARNYDEL
KQLTKNAIWEGKLIQEFIDFKENDLYRDMRILVVDGEVVGGYRRVSRDFRTNLYLGNVVEKLNIDEELEELALKCADLSE
AVILGVDILPTKDNYYVIELNSSPGTKGFRDIGINADKKIAEALVRYAKS

Sequences:

>Translated_290_residues
MFMVKITILSPEGRSCSVWSLKNEIEKLGAKCDIFLLSSPENLMSHDFKLETDLIHSRCGIGDYFDRLTLYSWQFINALE
VEGCRFINPIKTLYLTSDKFKCIKLLAKNKIKTPKTALIRDYEDAVKFIEKYNLRFPVVIKNSFSKCGLKVFMARNYDEL
KQLTKNAIWEGKLIQEFIDFKENDLYRDMRILVVDGEVVGGYRRVSRDFRTNLYLGNVVEKLNIDEELEELALKCADLSE
AVILGVDILPTKDNYYVIELNSSPGTKGFRDIGINADKKIAEALVRYAKS
>Mature_290_residues
MFMVKITILSPEGRSCSVWSLKNEIEKLGAKCDIFLLSSPENLMSHDFKLETDLIHSRCGIGDYFDRLTLYSWQFINALE
VEGCRFINPIKTLYLTSDKFKCIKLLAKNKIKTPKTALIRDYEDAVKFIEKYNLRFPVVIKNSFSKCGLKVFMARNYDEL
KQLTKNAIWEGKLIQEFIDFKENDLYRDMRILVVDGEVVGGYRRVSRDFRTNLYLGNVVEKLNIDEELEELALKCADLSE
AVILGVDILPTKDNYYVIELNSSPGTKGFRDIGINADKKIAEALVRYAKS

Specific function: Catalyzes the ATP-dependent addition of one L-glutamate molecule to gamma-F420-2, producing the mature coenzyme alpha- F420-3 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=232, Percent_Identity=28.448275862069, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33391; Mature: 33391

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMVKITILSPEGRSCSVWSLKNEIEKLGAKCDIFLLSSPENLMSHDFKLETDLIHSRCG
CEEEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEEEECCHHHHHCCCEEHHHHHHHHCC
IGDYFDRLTLYSWQFINALEVEGCRFINPIKTLYLTSDKFKCIKLLAKNKIKTPKTALIR
CCHHHHHHHHHHHHHHCEEECCCCEEECCHHEEEECCCHHHHHHHHHHCCCCCCHHHHHH
DYEDAVKFIEKYNLRFPVVIKNSFSKCGLKVFMARNYDELKQLTKNAIWEGKLIQEFIDF
HHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHCC
KENDLYRDMRILVVDGEVVGGYRRVSRDFRTNLYLGNVVEKLNIDEELEELALKCADLSE
CCCCCCCCEEEEEECCHHHCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCC
AVILGVDILPTKDNYYVIELNSSPGTKGFRDIGINADKKIAEALVRYAKS
EEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFMVKITILSPEGRSCSVWSLKNEIEKLGAKCDIFLLSSPENLMSHDFKLETDLIHSRCG
CEEEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEEEECCHHHHHCCCEEHHHHHHHHCC
IGDYFDRLTLYSWQFINALEVEGCRFINPIKTLYLTSDKFKCIKLLAKNKIKTPKTALIR
CCHHHHHHHHHHHHHHCEEECCCCEEECCHHEEEECCCHHHHHHHHHHCCCCCCHHHHHH
DYEDAVKFIEKYNLRFPVVIKNSFSKCGLKVFMARNYDELKQLTKNAIWEGKLIQEFIDF
HHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHCC
KENDLYRDMRILVVDGEVVGGYRRVSRDFRTNLYLGNVVEKLNIDEELEELALKCADLSE
CCCCCCCCEEEEEECCHHHCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCC
AVILGVDILPTKDNYYVIELNSSPGTKGFRDIGINADKKIAEALVRYAKS
EEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]