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The map label for this gene is hisS

Identifier: 15669189

GI number: 15669189

Start: 928717

End: 929967

Strand: Direct

Name: hisS

Synonym: MJ1000

Alternate gene names: 15669189

Gene position: 928717-929967 (Clockwise)

Preceding gene: 15669186

Following gene: 15669190

Centisome position: 55.78

GC content: 33.65

Gene sequence:

>1251_bases
ATGATAGTGATGTTCCAAAAACCAAGAGGGACGAGAGATTTTTTACCAGAGGAGATGAAAAAAAGAAGATTTGTTGAAAA
TAAGCTAAGAGAGGTTTTTGAGAGGTATGGGTATAAGGAGATATTAACCCCAACCTTTGAAAGCTTTGAGTTAATAGCTA
AAAAAACAGGAGAAGAGATTAGAAAGCAGTTGTATGTGTTTAAGGACCATGGTGGGAGAGAAATGGCTTTAAGACCAGAG
ATGACATCCCCGGTTGTTAGATTCTATTTAAATGAATTGAAGAACCTACAAAAACCTTTAAGGCTTTATTATTTCGCTAA
TTGTTTTAGATATGAGAGACCTCAGGCAGGGAGGTTTAGAGAGTTTTGGCAGATGGGTTGTGAGTTAATAGGATGCAAAG
AACCATTGGCAGATGCTGAGGTTTTGAATTTAGCAATGGATGGATTGATAAATATTGGTTTGGATTTTGATGTTCATATA
GGGCATTTGGGAGTTTTGAAGGGAGTTTTAGAGAAATTTAATGTTAGTGAGGAAGAGGAGGTTAAAATAAGAAGATTAAT
TGATAAAGAGGATTATGATAATTTGAAGATTTATCTAACTCAAATCTTAGGAGAGGAGAAGAAAGAGCTAATATTTGAGA
TATTGAAGTTTAAAGGAAGTAGAGAGGTTTTGGATGAACTAAAGGAAATATTAAAGGACTTCCCAAAATCTATGGAGGCG
ATAAATAATTTGGAGGAGATTTTAGAGTTTGTTATTCATGATAAATATACAATAAACCTTGGAATTGCGAGAGGTTTAGA
TTACTACACAGGAATGGTATTTGAAATCTATGGGAAGAAGGGAGCTAAGCAGATATGTGGTGGCGGGAGATACGATAACT
TAATTGAAACGTTTGGAGGAGAACCAACTCCAGCTGTTGGTTTTGCCTATGGATTTGATAGGATTATGATGAATATCGAT
GATTTAGATATTGAGGAAGAGAGCATTTTAATAATCCCAGTAAAAAAAGATAAGGAATTAATTAAAAAATCTCTAATTAT
AGCTGATAAGTTAAGAAAAGCTGGAAAAATTGTAGAACTTGAGATTATGGGGAGAAAGCTAAGAAAAGCTTTAGATTATG
CAAACTCAAGAGGATTTAAGAAGGTAATTATTGTTGGAGAGAAAGAGCTTAATGAAGGGAAAGTAACTGTAAAAGATATG
ATAACTGGAGAGCAGAAATTAATTGGTATAGATGAATTGACAAATTTCTAA

Upstream 100 bases:

>100_bases
AAATATATAAATACTTATATCTTACAAAATTATGCTGATGAAACAAAGATTTATAAGATAGCTTTGCTATTTATTTACAA
AAAACAAAACGTATTTAAGG

Downstream 100 bases:

>100_bases
CCCCAATTTAAATCTTTATAAATTTTTATTTTTGGGATGTTTATGGTAAAAATAACCATACTATCACCAGAAGGAAGGAG
CTGTAGTGTATGGAGTTTAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MIVMFQKPRGTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEIRKQLYVFKDHGGREMALRPE
MTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFREFWQMGCELIGCKEPLADAEVLNLAMDGLINIGLDFDVHI
GHLGVLKGVLEKFNVSEEEEVKIRRLIDKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEA
INNLEEILEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGGEPTPAVGFAYGFDRIMMNID
DLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVELEIMGRKLRKALDYANSRGFKKVIIVGEKELNEGKVTVKDM
ITGEQKLIGIDELTNF

Sequences:

>Translated_416_residues
MIVMFQKPRGTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEIRKQLYVFKDHGGREMALRPE
MTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFREFWQMGCELIGCKEPLADAEVLNLAMDGLINIGLDFDVHI
GHLGVLKGVLEKFNVSEEEEVKIRRLIDKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEA
INNLEEILEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGGEPTPAVGFAYGFDRIMMNID
DLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVELEIMGRKLRKALDYANSRGFKKVIIVGEKELNEGKVTVKDM
ITGEQKLIGIDELTNF
>Mature_416_residues
MIVMFQKPRGTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEIRKQLYVFKDHGGREMALRPE
MTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFREFWQMGCELIGCKEPLADAEVLNLAMDGLINIGLDFDVHI
GHLGVLKGVLEKFNVSEEEEVKIRRLIDKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEA
INNLEEILEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGGEPTPAVGFAYGFDRIMMNID
DLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVELEIMGRKLRKALDYANSRGFKKVIIVGEKELNEGKVTVKDM
ITGEQKLIGIDELTNF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=437, Percent_Identity=29.2906178489703, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI15029520, Length=442, Percent_Identity=27.1493212669683, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1788861, Length=439, Percent_Identity=30.751708428246, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71993693, Length=449, Percent_Identity=26.7260579064588, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71993686, Length=449, Percent_Identity=26.7260579064588, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325290, Length=455, Percent_Identity=28.3516483516484, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24643061, Length=459, Percent_Identity=26.797385620915, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24643059, Length=478, Percent_Identity=25.7322175732218, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_METJA (Q58406)

Other databases:

- EMBL:   L77117
- PIR:   G64424
- RefSeq:   NP_247994.1
- ProteinModelPortal:   Q58406
- SMR:   Q58406
- GeneID:   1451897
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1000
- NMPDR:   fig|243232.1.peg.1031
- TIGR:   MJ1000
- HOGENOM:   HBG616575
- OMA:   YYFANCF
- ProtClustDB:   PRK00037
- BioCyc:   MJAN243232:MJ_1000-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48323; Mature: 48323

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVMFQKPRGTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEI
CEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHH
RKQLYVFKDHGGREMALRPEMTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFR
HHHHEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EFWQMGCELIGCKEPLADAEVLNLAMDGLINIGLDFDVHIGHLGVLKGVLEKFNVSEEEE
HHHHHCCEEECCCCCCCHHHHHHHHHHHHHEECCCEEEECCHHHHHHHHHHHCCCCCHHH
VKIRRLIDKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEA
HHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
INNLEEILEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGG
HHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHEEEECCCCHHHHCCCCCHHHHHHHHCC
EPTPAVGFAYGFDRIMMNIDDLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVEL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEE
EIMGRKLRKALDYANSRGFKKVIIVGEKELNEGKVTVKDMITGEQKLIGIDELTNF
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEHHHHHCCCHHEEEHHHHCCC
>Mature Secondary Structure
MIVMFQKPRGTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEI
CEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHH
RKQLYVFKDHGGREMALRPEMTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFR
HHHHEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EFWQMGCELIGCKEPLADAEVLNLAMDGLINIGLDFDVHIGHLGVLKGVLEKFNVSEEEE
HHHHHCCEEECCCCCCCHHHHHHHHHHHHHEECCCEEEECCHHHHHHHHHHHCCCCCHHH
VKIRRLIDKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEA
HHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
INNLEEILEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGG
HHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHEEEECCCCHHHHCCCCCHHHHHHHHCC
EPTPAVGFAYGFDRIMMNIDDLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVEL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEE
EIMGRKLRKALDYANSRGFKKVIIVGEKELNEGKVTVKDMITGEQKLIGIDELTNF
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEHHHHHCCCHHEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087