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The map label for this gene is mcrA

Identifier: 15669036

GI number: 15669036

Start: 771822

End: 773483

Strand: Direct

Name: mcrA

Synonym: MJ0846

Alternate gene names: 15669036

Gene position: 771822-773483 (Clockwise)

Preceding gene: 15669035

Following gene: 15669037

Centisome position: 46.36

GC content: 42.48

Gene sequence:

>1662_bases
ATGGATGCTGAAAAAAGATTGTTCTTAAAGGCATTAAAGGAAAAGTTTGAAGAAGACCCAAGAGAAAAATACACTAAGTT
CTATGTCTTTGGCGGATGGAGACAGTCAGCAAGAAAAAGAGAATTCGTTGAGGCAGCACAAAAATTAATTGAGAAGAGAG
GAGGAATTCCATTTTACAACCCAGATATTGGAGTTCCATTGGGGCAGAGAAAATTAATGCCTTACAAAGTTTCAAATACA
GATGCAATTGTTGAAGGGGATGACTTACACTTCATGAACAACGCTGCAATGCAGCAGTTCTGGGATGACATAAGAAGAAC
AGTTATCGTTGGGATGGATACAGCTCACGCTGTTCTTGAAAAGAGATTGGGGGTAGAGGTTACTCCAGAAACAATTAATG
AATACATGGAAACTATTAACCACGCTCTCCCAGGAGGAGCAGTTGTTCAGGAGCACATGGTTGAGGTCCACCCAGCATTA
GTCTGGGACTGTTACGCTAAGATATTCACTGGAGATGACGAATTAGCAGATGAGATTGACAAGAGGTTCTTAATTGACAT
TAACAAGTTGTTCCCAGAAGAGCAGGCAGAACAAATCAAGAAGGCAATCGGTAAGAGAACATACCAAGTTTCAAGAGTTC
CAACATTAGTCGGTAGAGTTTGTGATGGGGGAACAATAGCAAGATGGAGTGCTATGCAGATTGGAATGTCATTCATTACA
GCTTACAAGCTCTGTGCTGGGGAGGCAGCAATTGCTGACTTCTCATACGCTGCAAAGCACGCTGATGTCATTCAGATGGC
TTCATTCTTGCCAGCAAGAAGAGCAAGAGGGCCAAATGAACCAGGAGGTATCTTCTTCGGAGTCTTGGCAGATATTGTTC
AAACATCAAGAGTTTCAGATGACCCAGTTGAACAGTCATTAGAGGTTGTTGCTGCTGGGGCTATGTTGTATGACCAAATC
TGGTTAGGAGGATACATGTCTGGAGGAGTCGGATTTACACAGTATGCTACAGCAACCTATACAGATGACATCTTGGATGA
CTTCTCATACTACGGATATGACTACATAACCAAGAAATATGGAGGATGCAACAGCGTAAAACCAACAATGGATGTTGTTG
AAGATATTGCTACTGAAGTAACTTTATATGGTTTAGAGCAGTATGACACCTTCCCAGCATTGTTAGAAGACCACTTCGGA
GGTTCCCAAAGAGCAGGGGTTACAGCTGCTGCAGCAGGTATTACAACTGCATTAGCTACAGGAAACTCAAACGCTGGAGT
TAACGGATGGTATCTAAGCCAGATATTGCACAAAGAATACCACAGCAGATTAGGATTCTATGGTTATGACTTACAAGACC
AGTGTGGAGCAGCCAACTCATTATCATTCAGAAACGATGAAGGTTCCCCATTAGAATTGAGAGGGCCTAACTATCCAAAC
TACGCAATGAACGTTGGTCACCAAGGAGAATATGCTGGAATTACACAGGCTGCACACTCAGCAAGAGGAGACGCATTTGC
ATTGAACCCATTAATTAAGGTTGCATTTGCAGACCCATCATTAGTCTTTGACTTCACACATCCAAGAAAAGAGTTTGCAA
GAGGTGCTTTAAGAGAATTCGAGCCAGCTGGAGAAAGAGATCCAATCATCCCAGCTCACTAA

Upstream 100 bases:

>100_bases
CTTTCTCCCACTAAAACTACAAAAATAAATTATAAATCTCAAAAATATAACTTAGAAAAATAAAAAATAAAAATCAAAAT
TCCTCACAGAGGTGAGCCCG

Downstream 100 bases:

>100_bases
ATTCTAAATTTTTTAATTTTTTATTATGTTGATATTGTTAAGCATATTAAAACGTAAAATACAAAAACTTAAATTCTGAC
ATTGTTTTTAATATTCTGGA

Product: methyl coenzyme M reductase I subunit McrA

Products: NA

Alternate protein names: MCR I alpha; Coenzyme-B sulfoethylthiotransferase alpha

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MDAEKRLFLKALKEKFEEDPREKYTKFYVFGGWRQSARKREFVEAAQKLIEKRGGIPFYNPDIGVPLGQRKLMPYKVSNT
DAIVEGDDLHFMNNAAMQQFWDDIRRTVIVGMDTAHAVLEKRLGVEVTPETINEYMETINHALPGGAVVQEHMVEVHPAL
VWDCYAKIFTGDDELADEIDKRFLIDINKLFPEEQAEQIKKAIGKRTYQVSRVPTLVGRVCDGGTIARWSAMQIGMSFIT
AYKLCAGEAAIADFSYAAKHADVIQMASFLPARRARGPNEPGGIFFGVLADIVQTSRVSDDPVEQSLEVVAAGAMLYDQI
WLGGYMSGGVGFTQYATATYTDDILDDFSYYGYDYITKKYGGCNSVKPTMDVVEDIATEVTLYGLEQYDTFPALLEDHFG
GSQRAGVTAAAAGITTALATGNSNAGVNGWYLSQILHKEYHSRLGFYGYDLQDQCGAANSLSFRNDEGSPLELRGPNYPN
YAMNVGHQGEYAGITQAAHSARGDAFALNPLIKVAFADPSLVFDFTHPRKEFARGALREFEPAGERDPIIPAH

Sequences:

>Translated_553_residues
MDAEKRLFLKALKEKFEEDPREKYTKFYVFGGWRQSARKREFVEAAQKLIEKRGGIPFYNPDIGVPLGQRKLMPYKVSNT
DAIVEGDDLHFMNNAAMQQFWDDIRRTVIVGMDTAHAVLEKRLGVEVTPETINEYMETINHALPGGAVVQEHMVEVHPAL
VWDCYAKIFTGDDELADEIDKRFLIDINKLFPEEQAEQIKKAIGKRTYQVSRVPTLVGRVCDGGTIARWSAMQIGMSFIT
AYKLCAGEAAIADFSYAAKHADVIQMASFLPARRARGPNEPGGIFFGVLADIVQTSRVSDDPVEQSLEVVAAGAMLYDQI
WLGGYMSGGVGFTQYATATYTDDILDDFSYYGYDYITKKYGGCNSVKPTMDVVEDIATEVTLYGLEQYDTFPALLEDHFG
GSQRAGVTAAAAGITTALATGNSNAGVNGWYLSQILHKEYHSRLGFYGYDLQDQCGAANSLSFRNDEGSPLELRGPNYPN
YAMNVGHQGEYAGITQAAHSARGDAFALNPLIKVAFADPSLVFDFTHPRKEFARGALREFEPAGERDPIIPAH
>Mature_553_residues
MDAEKRLFLKALKEKFEEDPREKYTKFYVFGGWRQSARKREFVEAAQKLIEKRGGIPFYNPDIGVPLGQRKLMPYKVSNT
DAIVEGDDLHFMNNAAMQQFWDDIRRTVIVGMDTAHAVLEKRLGVEVTPETINEYMETINHALPGGAVVQEHMVEVHPAL
VWDCYAKIFTGDDELADEIDKRFLIDINKLFPEEQAEQIKKAIGKRTYQVSRVPTLVGRVCDGGTIARWSAMQIGMSFIT
AYKLCAGEAAIADFSYAAKHADVIQMASFLPARRARGPNEPGGIFFGVLADIVQTSRVSDDPVEQSLEVVAAGAMLYDQI
WLGGYMSGGVGFTQYATATYTDDILDDFSYYGYDYITKKYGGCNSVKPTMDVVEDIATEVTLYGLEQYDTFPALLEDHFG
GSQRAGVTAAAAGITTALATGNSNAGVNGWYLSQILHKEYHSRLGFYGYDLQDQCGAANSLSFRNDEGSPLELRGPNYPN
YAMNVGHQGEYAGITQAAHSARGDAFALNPLIKVAFADPSLVFDFTHPRKEFARGALREFEPAGERDPIIPAH

Specific function: Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide

COG id: COG4058

COG function: function code H; Methyl coenzyme M reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MCRA_METJA (Q58256)

Other databases:

- EMBL:   L77117
- EMBL:   AF414040
- RefSeq:   NP_247840.1
- ProteinModelPortal:   Q58256
- SMR:   Q58256
- GeneID:   1451734
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0846
- NMPDR:   fig|243232.1.peg.877
- TIGR:   MJ0846
- HOGENOM:   HBG541009
- OMA:   AHSARGD
- ProtClustDB:   CLSK876061
- BioCyc:   MJAN243232:MJ_0846-MONOMER
- BRENDA:   2.8.4.1
- GO:   GO:0015948
- InterPro:   IPR022681
- InterPro:   IPR016212
- InterPro:   IPR008924
- InterPro:   IPR009047
- InterPro:   IPR003183
- InterPro:   IPR015811
- InterPro:   IPR015823
- InterPro:   IPR009024
- Gene3D:   G3DSA:1.20.840.10
- Gene3D:   G3DSA:3.90.390.10
- Gene3D:   G3DSA:3.30.70.470
- PIRSF:   PIRSF000262
- TIGRFAMs:   TIGR03256

Pfam domain/function: PF02249 MCR_alpha; PF02745 MCR_alpha_N; SSF48081 MCR_alpha_beta_C; SSF55088 MCR_fer_like

EC number: =2.8.4.1

Molecular weight: Translated: 61267; Mature: 61267

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAEKRLFLKALKEKFEEDPREKYTKFYVFGGWRQSARKREFVEAAQKLIEKRGGIPFYN
CCCHHHHHHHHHHHHHHCCHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDIGVPLGQRKLMPYKVSNTDAIVEGDDLHFMNNAAMQQFWDDIRRTVIVGMDTAHAVLE
CCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
KRLGVEVTPETINEYMETINHALPGGAVVQEHMVEVHPALVWDCYAKIFTGDDELADEID
HHCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRFLIDINKLFPEEQAEQIKKAIGKRTYQVSRVPTLVGRVCDGGTIARWSAMQIGMSFIT
HHHHHHHHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AYKLCAGEAAIADFSYAAKHADVIQMASFLPARRARGPNEPGGIFFGVLADIVQTSRVSD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
DPVEQSLEVVAAGAMLYDQIWLGGYMSGGVGFTQYATATYTDDILDDFSYYGYDYITKKY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
GGCNSVKPTMDVVEDIATEVTLYGLEQYDTFPALLEDHFGGSQRAGVTAAAAGITTALAT
CCCCCCCHHHHHHHHHHHHHHEECHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEC
GNSNAGVNGWYLSQILHKEYHSRLGFYGYDLQDQCGAANSLSFRNDEGSPLELRGPNYPN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCCC
YAMNVGHQGEYAGITQAAHSARGDAFALNPLIKVAFADPSLVFDFTHPRKEFARGALREF
HHCCCCCCCCCCHHHHHHHHCCCCEEEECCEEEEEECCCCEEEECCCCHHHHHHHHHHHC
EPAGERDPIIPAH
CCCCCCCCCCCCC
>Mature Secondary Structure
MDAEKRLFLKALKEKFEEDPREKYTKFYVFGGWRQSARKREFVEAAQKLIEKRGGIPFYN
CCCHHHHHHHHHHHHHHCCHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDIGVPLGQRKLMPYKVSNTDAIVEGDDLHFMNNAAMQQFWDDIRRTVIVGMDTAHAVLE
CCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
KRLGVEVTPETINEYMETINHALPGGAVVQEHMVEVHPALVWDCYAKIFTGDDELADEID
HHCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRFLIDINKLFPEEQAEQIKKAIGKRTYQVSRVPTLVGRVCDGGTIARWSAMQIGMSFIT
HHHHHHHHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AYKLCAGEAAIADFSYAAKHADVIQMASFLPARRARGPNEPGGIFFGVLADIVQTSRVSD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
DPVEQSLEVVAAGAMLYDQIWLGGYMSGGVGFTQYATATYTDDILDDFSYYGYDYITKKY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
GGCNSVKPTMDVVEDIATEVTLYGLEQYDTFPALLEDHFGGSQRAGVTAAAAGITTALAT
CCCCCCCHHHHHHHHHHHHHHEECHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEC
GNSNAGVNGWYLSQILHKEYHSRLGFYGYDLQDQCGAANSLSFRNDEGSPLELRGPNYPN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCCC
YAMNVGHQGEYAGITQAAHSARGDAFALNPLIKVAFADPSLVFDFTHPRKEFARGALREF
HHCCCCCCCCCCHHHHHHHHCCCCEEEECCEEEEEECCCCEEEECCCCHHHHHHHHHHHC
EPAGERDPIIPAH
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 12427943