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The map label for this gene is cofE

Identifier: 15668949

GI number: 15668949

Start: 689588

End: 690337

Strand: Direct

Name: cofE

Synonym: MJ0768

Alternate gene names: 15668949

Gene position: 689588-690337 (Clockwise)

Preceding gene: 15668910

Following gene: 15668952

Centisome position: 41.42

GC content: 35.2

Gene sequence:

>750_bases
ATGATTAAAGAAAAAAGAAAGGTAGAGGTTATTGGCTTAGAACTCCCAATTTTTAAAGGTGGAGAACAAATAAATTTATC
TGAGTTGATAGCTCAATACCCAATTGAAGATGGAGATATTATTGTAATAGCAGAAACATTAATCTCAAAATTAGAGGGTG
GAGTTATAGATAGGGATAAAATAATCCCTTCAAAAGAAGCAATTGAATTAGCCAAAAAAACTGGAAAAGACCCGAAGGTT
GTGCAAGTTATATTAGATGAGGCTAAAGAAATAGTTAAGGTTGGAAAGAACTTCATTATAACAGAAACCAAACATGGATT
TGTTTGTGCTAACAGTGGAGTTGATGAAAGTAACATATACAAAGGCATAAAAATTCTACCAAAAAATCCAGATGAAAGTG
CTGAAAAGATTAGGAAAGAGATTGAAAAATTAACTGGAAAGAGAGTTGGAGTAATAATATCAGATAGTGTTGGAAGACCT
TTCAGGAAGGGAGCTGTTGGAATAGCAATAGGAGTTAGTGGAATCTTAGCATTATGGGATAGGAAGGGGGAAAAGGATTT
GTTTGGTAGGGAGTTAAAAACAACTGAAGTTGCCATTGCTGATGAGTTGGCAAGTATGGCAAATGTGGTTATGGGAGAGG
CTGATGAGGGCATTCCAGTTGTAATAATTAGGGGGGCTAATGTGCCTTTTGGTAATGGGAAGGGAAGAGATTTAATTAGA
CCTAAGGAGGAAGATGTTTTTAGAAATTAA

Upstream 100 bases:

>100_bases
TCTCAACATTAACCATACTATCACATAAAAAACTATTTTTATTCTAACTTAAAACATTTATTGTAATTATATATAATTTC
TTAAAAAGGGATGTGATAGA

Downstream 100 bases:

>100_bases
TTTATATATTTTTATTTTTTAAGTTTAATGTTGGACTTTTGTGCAAAAGTCTAACATTGGTTAAAAATTTTCGGACAAAA
ATACATAAAATCGATTATCT

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDKIIPSKEAIELAKKTGKDPKV
VQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIYKGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRP
FRKGAVGIAIGVSGILALWDRKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIR
PKEEDVFRN

Sequences:

>Translated_249_residues
MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDKIIPSKEAIELAKKTGKDPKV
VQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIYKGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRP
FRKGAVGIAIGVSGILALWDRKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIR
PKEEDVFRN
>Mature_249_residues
MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDKIIPSKEAIELAKKTGKDPKV
VQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIYKGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRP
FRKGAVGIAIGVSGILALWDRKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIR
PKEEDVFRN

Specific function: Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8- hydroxy-5-deazariboflavin (F420-0) to form F420-0-glutamyl- glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFE_METJA (Q58178)

Other databases:

- EMBL:   L77117
- PIR:   H64395
- RefSeq:   NP_247753.1
- ProteinModelPortal:   Q58178
- GeneID:   1451645
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0768
- NMPDR:   fig|243232.1.peg.790
- TIGR:   MJ0768
- HOGENOM:   HBG578188
- OMA:   GEKDLFG
- ProtClustDB:   PRK13293
- BioCyc:   MetaCyc:MONOMER-12187
- BioCyc:   MJAN243232:MJ_0768-MONOMER
- HAMAP:   MF_01258
- InterPro:   IPR002847
- InterPro:   IPR008225
- TIGRFAMs:   TIGR01916

Pfam domain/function: PF01996 F420_ligase

EC number: NA

Molecular weight: Translated: 27148; Mature: 27148

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDK
CCCCCCEEEEEEEEECEECCCCEECHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCHHC
IIPSKEAIELAKKTGKDPKVVQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIY
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCHHHHH
KGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRPFRKGAVGIAIGVSGILALWD
CCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHCCCEEEEEHHHHHHHHHC
RKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIR
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PKEEDVFRN
CCHHHHCCC
>Mature Secondary Structure
MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDK
CCCCCCEEEEEEEEECEECCCCEECHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCHHC
IIPSKEAIELAKKTGKDPKVVQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIY
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCHHHHH
KGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRPFRKGAVGIAIGVSGILALWD
CCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHCCCEEEEEHHHHHHHHHC
RKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIR
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PKEEDVFRN
CCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087