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The map label for this gene is aroA

Identifier: 15668679

GI number: 15668679

Start: 443159

End: 444448

Strand: Direct

Name: aroA

Synonym: MJ0502

Alternate gene names: 15668679

Gene position: 443159-444448 (Clockwise)

Preceding gene: 15668673

Following gene: 15668680

Centisome position: 26.62

GC content: 33.18

Gene sequence:

>1290_bases
ATGTATTTGCTGATTGTTAAAAAAACAGATAGATTGGAAGGGATAGTTAAAGCTCCTCCTTCAAAATCTTACACTCATAG
AGCAGTTATTGGAGCATCTTTAGCTGATGGAGTTAGCAGAATAATAAACCCACTTTGGGGAGCTGATTGTTTATCATCTG
TTCATGGTTGTAGAATGTTGGGGGCTAATATTGAGTTAGATAAAGAAAAAGATGAATGGATTGTTAAAGGAGGAGAGTTA
AAAACCCCAGATAATATCATAGATATTGGGAACAGTGGAACAACCTTAAGGATTTTAACCTCTATAGCCTCACAGATACC
AAAAGGATATGCAATTTTAACTGGAGATGATTCTATAAGAAAGAGACCGATGCAACCTTTATTAGATGCCTTAAAACAGC
TAAATATAGAGGCATTTTCATCAAAATTAGATGGAACTGCACCAATAATAGTTAAAAGTGGGAAAATTTATGGAAATGTG
GTAAAGATTAGAGGAGACATTAGCTCTCAGTTTATAACCTCTTTGATGATGCTCCTTCCATTTAATAAAGAAGATACTGA
GATAATTCTAACCTCACCACTAAAATCAAAGCCATATATTGATATAACATTGGATATATTAAATAAATTTGGAATAAAGA
TTGATAAAACTGATAACGGCTTTTTAGTCTATGGAAATCAGAAGTATAAACCAATAGATTATATTGTTGAGGGAGATTAT
TCTTCAGCTTCATACTTAATAGCTGCTGGAGTTTTAATAAACTCAAATATAACTATTGAAAACCTATTTGCCAACTCAAA
GCAAGGAGATAAAGCTATAATCAATATTGTCAAAGAGATGGGAGCAGATATTAAAGTTAAAAAAGATAAAGTAATTATTG
AAGGAGAATACAGCTTAAAAGGAATAGATGTTGATGTTAAAGATATTCCAGATTTAGTCCCAACAATTGCAGTTCTTGGA
TGCTTTGCAGAAGGAAAGACTGAGATTTACAATGGAGAACATGTTAGATTAAAGGAGTGCGATAGATTAAGAGCTTGTGC
TGTTGAATTAAAAAAGATGGGTGCTGATATTGAAGAGAAACCAGATGGTTTAATTATAAGAGGAGTTAAAAAGCTAAAGG
GGGCTAAGCTAAACACCTATCACGACCATAGATTGGTTATGGCATTTACTATAGCTGGTTTAAAGGCAGAAGGAGAAACA
ATTATTGAAGGAGAGGAGGCCGTTAAAATCTCTTTCCCAAACTTTGTTGATGTTATGAAGAGCTTAGGGGCTAATATAGA
GGTAAAATAA

Upstream 100 bases:

>100_bases
TATAGAATTATCATAATTTCATTTGTATTAACTGTGTAATTATAACAACCTTAATATATCACATTTAAATTATACTCTGT
TGATTAAAAAATTAGTGGTG

Downstream 100 bases:

>100_bases
AAAGTAAGTGAATAAATCAATCTCTTCATATTGATATTTTGATTAAAAAATAATAAAAAAATTTATACAGGTAATAACAA
CTCTATGACAAAAGTGCTGG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MYLLIVKKTDRLEGIVKAPPSKSYTHRAVIGASLADGVSRIINPLWGADCLSSVHGCRMLGANIELDKEKDEWIVKGGEL
KTPDNIIDIGNSGTTLRILTSIASQIPKGYAILTGDDSIRKRPMQPLLDALKQLNIEAFSSKLDGTAPIIVKSGKIYGNV
VKIRGDISSQFITSLMMLLPFNKEDTEIILTSPLKSKPYIDITLDILNKFGIKIDKTDNGFLVYGNQKYKPIDYIVEGDY
SSASYLIAAGVLINSNITIENLFANSKQGDKAIINIVKEMGADIKVKKDKVIIEGEYSLKGIDVDVKDIPDLVPTIAVLG
CFAEGKTEIYNGEHVRLKECDRLRACAVELKKMGADIEEKPDGLIIRGVKKLKGAKLNTYHDHRLVMAFTIAGLKAEGET
IIEGEEAVKISFPNFVDVMKSLGANIEVK

Sequences:

>Translated_429_residues
MYLLIVKKTDRLEGIVKAPPSKSYTHRAVIGASLADGVSRIINPLWGADCLSSVHGCRMLGANIELDKEKDEWIVKGGEL
KTPDNIIDIGNSGTTLRILTSIASQIPKGYAILTGDDSIRKRPMQPLLDALKQLNIEAFSSKLDGTAPIIVKSGKIYGNV
VKIRGDISSQFITSLMMLLPFNKEDTEIILTSPLKSKPYIDITLDILNKFGIKIDKTDNGFLVYGNQKYKPIDYIVEGDY
SSASYLIAAGVLINSNITIENLFANSKQGDKAIINIVKEMGADIKVKKDKVIIEGEYSLKGIDVDVKDIPDLVPTIAVLG
CFAEGKTEIYNGEHVRLKECDRLRACAVELKKMGADIEEKPDGLIIRGVKKLKGAKLNTYHDHRLVMAFTIAGLKAEGET
IIEGEEAVKISFPNFVDVMKSLGANIEVK
>Mature_429_residues
MYLLIVKKTDRLEGIVKAPPSKSYTHRAVIGASLADGVSRIINPLWGADCLSSVHGCRMLGANIELDKEKDEWIVKGGEL
KTPDNIIDIGNSGTTLRILTSIASQIPKGYAILTGDDSIRKRPMQPLLDALKQLNIEAFSSKLDGTAPIIVKSGKIYGNV
VKIRGDISSQFITSLMMLLPFNKEDTEIILTSPLKSKPYIDITLDILNKFGIKIDKTDNGFLVYGNQKYKPIDYIVEGDY
SSASYLIAAGVLINSNITIENLFANSKQGDKAIINIVKEMGADIKVKKDKVIIEGEYSLKGIDVDVKDIPDLVPTIAVLG
CFAEGKTEIYNGEHVRLKECDRLRACAVELKKMGADIEEKPDGLIIRGVKKLKGAKLNTYHDHRLVMAFTIAGLKAEGET
IIEGEEAVKISFPNFVDVMKSLGANIEVK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=425, Percent_Identity=35.5294117647059, Blast_Score=221, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6320332, Length=462, Percent_Identity=29.004329004329, Blast_Score=171, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_METJA (Q57925)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247478.1
- ProteinModelPortal:   Q57925
- GeneID:   1451364
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0502
- NMPDR:   fig|243232.1.peg.515
- TIGR:   MJ0502
- HOGENOM:   HBG646626
- OMA:   SSQFITS
- ProtClustDB:   PRK02427
- BioCyc:   MJAN243232:MJ_0502-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47009; Mature: 47009

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLLIVKKTDRLEGIVKAPPSKSYTHRAVIGASLADGVSRIINPLWGADCLSSVHGCRML
CEEEEEECCCCCCCEEECCCCCCCCHHHEEHHHHHHHHHHHHHHHCCHHHHHHHCCCEEE
GANIELDKEKDEWIVKGGELKTPDNIIDIGNSGTTLRILTSIASQIPKGYAILTGDDSIR
CCCEEECCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
KRPMQPLLDALKQLNIEAFSSKLDGTAPIIVKSGKIYGNVVKIRGDISSQFITSLMMLLP
HCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHC
FNKEDTEIILTSPLKSKPYIDITLDILNKFGIKIDKTDNGFLVYGNQKYKPIDYIVEGDY
CCCCCCEEEEECCCCCCCEEEEEHHHHHHCCCEEEECCCCEEEECCCCCCCEEEEEECCC
SSASYLIAAGVLINSNITIENLFANSKQGDKAIINIVKEMGADIKVKKDKVIIEGEYSLK
CCCCEEEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCEEEEECCCEEE
GIDVDVKDIPDLVPTIAVLGCFAEGKTEIYNGEHVRLKECDRLRACAVELKKMGADIEEK
EEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCC
PDGLIIRGVKKLKGAKLNTYHDHRLVMAFTIAGLKAEGETIIEGEEAVKISFPNFVDVMK
CCCEEEEEHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCEEEEECCHHHHHHH
SLGANIEVK
HCCCCEEEC
>Mature Secondary Structure
MYLLIVKKTDRLEGIVKAPPSKSYTHRAVIGASLADGVSRIINPLWGADCLSSVHGCRML
CEEEEEECCCCCCCEEECCCCCCCCHHHEEHHHHHHHHHHHHHHHCCHHHHHHHCCCEEE
GANIELDKEKDEWIVKGGELKTPDNIIDIGNSGTTLRILTSIASQIPKGYAILTGDDSIR
CCCEEECCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
KRPMQPLLDALKQLNIEAFSSKLDGTAPIIVKSGKIYGNVVKIRGDISSQFITSLMMLLP
HCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHC
FNKEDTEIILTSPLKSKPYIDITLDILNKFGIKIDKTDNGFLVYGNQKYKPIDYIVEGDY
CCCCCCEEEEECCCCCCCEEEEEHHHHHHCCCEEEECCCCEEEECCCCCCCEEEEEECCC
SSASYLIAAGVLINSNITIENLFANSKQGDKAIINIVKEMGADIKVKKDKVIIEGEYSLK
CCCCEEEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCEEEEECCCEEE
GIDVDVKDIPDLVPTIAVLGCFAEGKTEIYNGEHVRLKECDRLRACAVELKKMGADIEEK
EEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCC
PDGLIIRGVKKLKGAKLNTYHDHRLVMAFTIAGLKAEGETIIEGEEAVKISFPNFVDVMK
CCCEEEEEHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCEEEEECCHHHHHHH
SLGANIEVK
HCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087