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The map label for this gene is speB [C]

Identifier: 15668484

GI number: 15668484

Start: 289278

End: 290132

Strand: Reverse

Name: speB [C]

Synonym: MJ0309

Alternate gene names: 15668484

Gene position: 290132-289278 (Counterclockwise)

Preceding gene: 15668485

Following gene: 15668483

Centisome position: 17.43

GC content: 32.75

Gene sequence:

>855_bases
ATGGAGGAGCACTTTATTGATTTATCTAAATTTATGATGGCAAATTGTCCTTATGAAGAAGCTGAGGGAGTTATATTTTC
AATTCCCTATGATGAAACAACCTCATTTAAACCGGGAGCGAGAGAGGGAGGAAACGCTATAAGAACAGCATCATGGGGTT
TAGAAACATACAGCCCAATTTTAGATAGAGATTTGGCAGAATTAAAATACTGTGATTTAAAAGATTTAGATTTGTATGGA
AGTCAAGAAGAAATATTTGGCACAATTCACTCAGTCTCAAGGGAAATATTAAAAGAAAATAAAAAAATCATTGTTTTTGG
AGGAGAGCATTCTATAACTTATCCAATAATCAAAGCTGTAAAAGACATCTATGATGATTTTATTGTTATTCAATTTGATG
CCCATTGTGATTTGAGAGATGAATATTTGGGTAATAAGCTCTCTCATGCGTGTGTTATGAGGAGAGTTTATGAGCTAACC
AAAAATATATTCCAATTTGGAATTAGAAGTGGAGATAAAGAGGAATGGGACCTTGCAAGGAAAAACAACCTCTATCTAAA
GATGGATCTAATGAATAAGGATGATTTAGAATATATAAAGAGCTTAGACAAGCCAATATATGTAACTATAGATATCGATG
TGTTAGACCCTGCCTATGCTCCAGGAACTGGAACTCCAGAACCCTGTGGATTTTCAACAAGAGAACTTTTTAACTCTTTA
TATTTATTAGAAGAGGTTAAAGATAAAATTATTGGTTTTGATATAGTTGAAGTTTCTCCGATTTATGATATTGCCAATAT
TACAGCAATAACCGCTGCTAAAATAGCAAGAGAACTTATGTTGATGATTCTATAA

Upstream 100 bases:

>100_bases
TAAACAATCTTTTTTATTTTTAATGATTAGGATATATATTTTTATATGTGCTATGGTTTTTATTCTTCTTTTAGTATATT
TTCTTTGTTGAGGTGGTTAA

Downstream 100 bases:

>100_bases
CTAATTTTGAGCATATGTGTTAAAGTTATATTTTTCTGCTTATACTTCTAATTCATATGATTTTTTATTGTTTTTGGTGA
AAGGCTATGATATGATTTAA

Product: agmatinase SpeB

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MEEHFIDLSKFMMANCPYEEAEGVIFSIPYDETTSFKPGAREGGNAIRTASWGLETYSPILDRDLAELKYCDLKDLDLYG
SQEEIFGTIHSVSREILKENKKIIVFGGEHSITYPIIKAVKDIYDDFIVIQFDAHCDLRDEYLGNKLSHACVMRRVYELT
KNIFQFGIRSGDKEEWDLARKNNLYLKMDLMNKDDLEYIKSLDKPIYVTIDIDVLDPAYAPGTGTPEPCGFSTRELFNSL
YLLEEVKDKIIGFDIVEVSPIYDIANITAITAAKIARELMLMIL

Sequences:

>Translated_284_residues
MEEHFIDLSKFMMANCPYEEAEGVIFSIPYDETTSFKPGAREGGNAIRTASWGLETYSPILDRDLAELKYCDLKDLDLYG
SQEEIFGTIHSVSREILKENKKIIVFGGEHSITYPIIKAVKDIYDDFIVIQFDAHCDLRDEYLGNKLSHACVMRRVYELT
KNIFQFGIRSGDKEEWDLARKNNLYLKMDLMNKDDLEYIKSLDKPIYVTIDIDVLDPAYAPGTGTPEPCGFSTRELFNSL
YLLEEVKDKIIGFDIVEVSPIYDIANITAITAAKIARELMLMIL
>Mature_284_residues
MEEHFIDLSKFMMANCPYEEAEGVIFSIPYDETTSFKPGAREGGNAIRTASWGLETYSPILDRDLAELKYCDLKDLDLYG
SQEEIFGTIHSVSREILKENKKIIVFGGEHSITYPIIKAVKDIYDDFIVIQFDAHCDLRDEYLGNKLSHACVMRRVYELT
KNIFQFGIRSGDKEEWDLARKNNLYLKMDLMNKDDLEYIKSLDKPIYVTIDIDVLDPAYAPGTGTPEPCGFSTRELFNSL
YLLEEVKDKIIGFDIVEVSPIYDIANITAITAAKIARELMLMIL

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family

Homologues:

Organism=Homo sapiens, GI37537722, Length=281, Percent_Identity=32.0284697508897, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1789306, Length=285, Percent_Identity=32.6315789473684, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y309_METJA (Q57757)

Other databases:

- EMBL:   L77117
- PIR:   F64338
- RefSeq:   NP_247282.1
- ProteinModelPortal:   Q57757
- SMR:   Q57757
- GeneID:   1451164
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0309
- NMPDR:   fig|243232.1.peg.319
- TIGR:   MJ0309
- HOGENOM:   HBG391953
- OMA:   KDIFQFG
- ProtClustDB:   CLSK876153
- BioCyc:   MJAN243232:MJ_0309-MONOMER
- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855
- Gene3D:   G3DSA:3.40.800.10
- PANTHER:   PTHR11358
- PRINTS:   PR00116
- TIGRFAMs:   TIGR01230

Pfam domain/function: PF00491 Arginase

EC number: 3.5.3.11

Molecular weight: Translated: 32478; Mature: 32478

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01053 ARGINASE_1; PS51409 ARGINASE_2; PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEHFIDLSKFMMANCPYEEAEGVIFSIPYDETTSFKPGAREGGNAIRTASWGLETYSPI
CCCHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
LDRDLAELKYCDLKDLDLYGSQEEIFGTIHSVSREILKENKKIIVFGGEHSITYPIIKAV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
KDIYDDFIVIQFDAHCDLRDEYLGNKLSHACVMRRVYELTKNIFQFGIRSGDKEEWDLAR
HHHHCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KNNLYLKMDLMNKDDLEYIKSLDKPIYVTIDIDVLDPAYAPGTGTPEPCGFSTRELFNSL
CCCEEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
YLLEEVKDKIIGFDIVEVSPIYDIANITAITAAKIARELMLMIL
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEHFIDLSKFMMANCPYEEAEGVIFSIPYDETTSFKPGAREGGNAIRTASWGLETYSPI
CCCHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
LDRDLAELKYCDLKDLDLYGSQEEIFGTIHSVSREILKENKKIIVFGGEHSITYPIIKAV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
KDIYDDFIVIQFDAHCDLRDEYLGNKLSHACVMRRVYELTKNIFQFGIRSGDKEEWDLAR
HHHHCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KNNLYLKMDLMNKDDLEYIKSLDKPIYVTIDIDVLDPAYAPGTGTPEPCGFSTRELFNSL
CCCEEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
YLLEEVKDKIIGFDIVEVSPIYDIANITAITAAKIARELMLMIL
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087