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The map label for this gene is hycB [C]

Identifier: 15668439

GI number: 15668439

Start: 252425

End: 252925

Strand: Reverse

Name: hycB [C]

Synonym: MJ0265

Alternate gene names: 15668439

Gene position: 252925-252425 (Counterclockwise)

Preceding gene: 15668440

Following gene: 15668438

Centisome position: 15.19

GC content: 34.93

Gene sequence:

>501_bases
ATGGTGATGAAAAAAATAATCATGACAAACTTCAACTGTGATAACTGTGGGGATTGTGTTAAGGCATGCATGGAGAAGAA
TAAAGTTGGAAGAATTGCCATAATGGAGAAAGATGGCAAATACATTCCAATTGTCTGCCAACACTGTGCTTCAGCTCCTT
GTAAGGAAGTCTGCCCAGTTTCAGCAATTGAACATAAAGACGGCTACGTCTATTTAAATGAAGATGTTTGTATTGGTTGT
GGTTTATGTGCTTTAGCATGTCCATTTGGAGCTATATTGATGGAGGATAAAGCATACAAGTGTATTTTATGCAATGGAGA
TGAACCAGCATGTGTTAAAGCTTGCTCAAAGAGATGCTTAGAGCTTGTTGATGTAAATGAGTTAATATTTGCTAAGAGGG
ATAAGTCTTTAGATTTATTTAGTAAGATGTCTCTTCCTACACAAAAATCAGATAACAGTTTAATTTCAAAAATAACAATA
GACGCAAAAGTTAAACCTTAA

Upstream 100 bases:

>100_bases
GGATATTGAGAGAATTCAAAAATATATTGATGAGAAATGTAAGTTGTTAGGATTGTAATTAAAATTTCTTTTTTTACTAA
AATTAAAATAGTTTTTGGTG

Downstream 100 bases:

>100_bases
ATTGTTGTAATATTATAACTTTTTATCTTTTTTAATCCCTTATGCACCAAGTGAAGAGTTTTCTTTTATTGGCTATTGAT
GGTGAAAAAATGGTTGTAGT

Product: carbon monoxide dehydrogenase iron sulfur subunit CooF2

Products: CO2; AH2

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MVMKKIIMTNFNCDNCGDCVKACMEKNKVGRIAIMEKDGKYIPIVCQHCASAPCKEVCPVSAIEHKDGYVYLNEDVCIGC
GLCALACPFGAILMEDKAYKCILCNGDEPACVKACSKRCLELVDVNELIFAKRDKSLDLFSKMSLPTQKSDNSLISKITI
DAKVKP

Sequences:

>Translated_166_residues
MVMKKIIMTNFNCDNCGDCVKACMEKNKVGRIAIMEKDGKYIPIVCQHCASAPCKEVCPVSAIEHKDGYVYLNEDVCIGC
GLCALACPFGAILMEDKAYKCILCNGDEPACVKACSKRCLELVDVNELIFAKRDKSLDLFSKMSLPTQKSDNSLISKITI
DAKVKP
>Mature_166_residues
MVMKKIIMTNFNCDNCGDCVKACMEKNKVGRIAIMEKDGKYIPIVCQHCASAPCKEVCPVSAIEHKDGYVYLNEDVCIGC
GLCALACPFGAILMEDKAYKCILCNGDEPACVKACSKRCLELVDVNELIFAKRDKSLDLFSKMSLPTQKSDNSLISKITI
DAKVKP

Specific function: Probable Electron Transfer Protein For Hydrogenase 3. [C]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1789079, Length=194, Percent_Identity=31.4432989690722, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI87082114, Length=181, Percent_Identity=29.8342541436464, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1788811, Length=145, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=3e-18,
Organism=Escherichia coli, GI87082180, Length=98, Percent_Identity=42.8571428571429, Blast_Score=83, Evalue=7e-18,
Organism=Escherichia coli, GI2367245, Length=107, Percent_Identity=38.3177570093458, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082179, Length=153, Percent_Identity=31.3725490196078, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1787122, Length=115, Percent_Identity=40, Blast_Score=73, Evalue=8e-15,
Organism=Escherichia coli, GI1787872, Length=115, Percent_Identity=39.1304347826087, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1789067, Length=133, Percent_Identity=35.3383458646617, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI2367345, Length=139, Percent_Identity=33.8129496402878, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FER9_METJA (Q57713)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247237.1
- ProteinModelPortal:   Q57713
- GeneID:   1451119
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0265
- NMPDR:   fig|243232.1.peg.274
- TIGR:   MJ0265
- HOGENOM:   HBG726839
- OMA:   SCAMDAN
- ProtClustDB:   CLSK876136
- BioCyc:   MJAN243232:MJ_0265-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: 1.2.99.2

Molecular weight: Translated: 18229; Mature: 18229

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
14.5 %Cys+Met (Translated Protein)
10.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
14.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMKKIIMTNFNCDNCGDCVKACMEKNKVGRIAIMEKDGKYIPIVCQHCASAPCKEVCPV
CCCHHEEHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHCCCCCHHHCCCC
SAIEHKDGYVYLNEDVCIGCGLCALACPFGAILMEDKAYKCILCNGDEPACVKACSKRCL
CEEECCCCEEEECCCEEECCCHHHHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHH
ELVDVNELIFAKRDKSLDLFSKMSLPTQKSDNSLISKITIDAKVKP
HHHCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MVMKKIIMTNFNCDNCGDCVKACMEKNKVGRIAIMEKDGKYIPIVCQHCASAPCKEVCPV
CCCHHEEHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHCCCCCHHHCCCC
SAIEHKDGYVYLNEDVCIGCGLCALACPFGAILMEDKAYKCILCNGDEPACVKACSKRCL
CEEECCCCEEEECCCEEECCCHHHHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHH
ELVDVNELIFAKRDKSLDLFSKMSLPTQKSDNSLISKITIDAKVKP
HHHCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CO; H2O; A

Specific reaction: CO + H2O + A = CO2 + AH2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087