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The map label for this gene is ygfT [C]

Identifier: 15668438

GI number: 15668438

Start: 251922

End: 252383

Strand: Reverse

Name: ygfT [C]

Synonym: MJ0264

Alternate gene names: 15668438

Gene position: 252383-251922 (Counterclockwise)

Preceding gene: 15668439

Following gene: 15668437

Centisome position: 15.16

GC content: 31.82

Gene sequence:

>462_bases
ATGCACCAAGTGAAGAGTTTTCTTTTATTGGCTATTGATGGTGAAAAAATGGTTGTAGTAAATGTTGGGTCTTGCATTGG
ATGTAGGAGATGTGAAAGGAGTTGTCCAATAAATGGAATAACCTTCAATGAATTTCCAATAAAATGTATGCATTGTGATA
GAAATCCTTGTCTATATGCATGTCCGGAGAATGCAATAGAGAGGATTAATAACAAAGTGGTGGTTATAAAAGATAAGTGT
GTTGGTTGTGGTTTGTGTGCTTTAGCATGTCCATTTGGAGCTATAAGAATTGATGGAGTAGCGATAAAATGTAATGGATG
TTATAAAAGAGATGTTGAGATTTGCAAAGAAGTATGTCCAACAGGAGCTATTAACAACCTTGAAGAAATATTAAATAATA
AAATACAAAATACAGTGAATAAATTTAATAAGCTTTACTATCTTTATGCAAATGCAAAATAA

Upstream 100 bases:

>100_bases
AAAAATCAGATAACAGTTTAATTTCAAAAATAACAATAGACGCAAAAGTTAAACCTTAAATTGTTGTAATATTATAACTT
TTTATCTTTTTTAATCCCTT

Downstream 100 bases:

>100_bases
TTCCTTAATTTTCCTATTTTCGTAATTTTATAAGGTTCGAAAATTTACAATAAAACATATAAACCTATTTTTATTAATTG
TCCTTTTATCGAATCTTCAA

Product: carbon monoxide dehydrogenase iron sulfur subunit CooF1

Products: CO2; AH2

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MHQVKSFLLLAIDGEKMVVVNVGSCIGCRRCERSCPINGITFNEFPIKCMHCDRNPCLYACPENAIERINNKVVVIKDKC
VGCGLCALACPFGAIRIDGVAIKCNGCYKRDVEICKEVCPTGAINNLEEILNNKIQNTVNKFNKLYYLYANAK

Sequences:

>Translated_153_residues
MHQVKSFLLLAIDGEKMVVVNVGSCIGCRRCERSCPINGITFNEFPIKCMHCDRNPCLYACPENAIERINNKVVVIKDKC
VGCGLCALACPFGAIRIDGVAIKCNGCYKRDVEICKEVCPTGAINNLEEILNNKIQNTVNKFNKLYYLYANAK
>Mature_153_residues
MHQVKSFLLLAIDGEKMVVVNVGSCIGCRRCERSCPINGITFNEFPIKCMHCDRNPCLYACPENAIERINNKVVVIKDKC
VGCGLCALACPFGAIRIDGVAIKCNGCYKRDVEICKEVCPTGAINNLEEILNNKIQNTVNKFNKLYYLYANAK

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI87082180, Length=137, Percent_Identity=32.8467153284672, Blast_Score=89, Evalue=9e-20,
Organism=Escherichia coli, GI2367245, Length=152, Percent_Identity=29.6052631578947, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1788811, Length=146, Percent_Identity=26.7123287671233, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI87082179, Length=142, Percent_Identity=30.9859154929577, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1789079, Length=99, Percent_Identity=37.3737373737374, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1787872, Length=87, Percent_Identity=42.5287356321839, Blast_Score=63, Evalue=9e-12,
Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=27.7456647398844, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1787122, Length=87, Percent_Identity=41.3793103448276, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI87082114, Length=54, Percent_Identity=46.2962962962963, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y264_METJA (Q57712)

Other databases:

- EMBL:   L77117
- PIR:   A64333
- RefSeq:   NP_247236.1
- ProteinModelPortal:   Q57712
- GeneID:   1451118
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0264
- NMPDR:   fig|243232.1.peg.273
- TIGR:   MJ0264
- HOGENOM:   HBG726839
- OMA:   RRCERSC
- ProtClustDB:   CLSK2517563
- BioCyc:   MJAN243232:MJ_0264-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: 1.2.99.2

Molecular weight: Translated: 17013; Mature: 17013

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
12.4 %Cys+Met (Translated Protein)
10.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
12.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQVKSFLLLAIDGEKMVVVNVGSCIGCRRCERSCPINGITFNEFPIKCMHCDRNPCLYA
CCCCCEEEEEEECCCEEEEEECCCCCCHHHHHCCCCCCCEEECCCCEEEEEECCCCEEEE
CPENAIERINNKVVVIKDKCVGCGLCALACPFGAIRIDGVAIKCNGCYKRDVEICKEVCP
CCHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCEEECCEEEEECCCCHHHHHHHHHHCC
TGAINNLEEILNNKIQNTVNKFNKLYYLYANAK
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure
MHQVKSFLLLAIDGEKMVVVNVGSCIGCRRCERSCPINGITFNEFPIKCMHCDRNPCLYA
CCCCCEEEEEEECCCEEEEEECCCCCCHHHHHCCCCCCCEEECCCCEEEEEECCCCEEEE
CPENAIERINNKVVVIKDKCVGCGLCALACPFGAIRIDGVAIKCNGCYKRDVEICKEVCP
CCHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCEEECCEEEEECCCCHHHHHHHHHHCC
TGAINNLEEILNNKIQNTVNKFNKLYYLYANAK
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CO; H2O; A

Specific reaction: CO + H2O + A = CO2 + AH2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087