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The map label for this gene is thiD [C]

Identifier: 15668411

GI number: 15668411

Start: 226374

End: 227639

Strand: Direct

Name: thiD [C]

Synonym: MJ0236

Alternate gene names: 15668411

Gene position: 226374-227639 (Clockwise)

Preceding gene: 15668410

Following gene: 15668412

Centisome position: 13.6

GC content: 31.12

Gene sequence:

>1266_bases
TTGGTGATTTTAATGGTTATTTTAGCTATTGGTGGCTACGACCCTACAAGTGGAGCTGGCATCTCTGCTGATATAAAAAC
AGCCCATACATTGGGAGTTTATTGCCCAACAATAACAACATCAGTAATTCCTCAAAATAATAAAATGGTTTATGAAAAGT
TTGATTTACCAGAGGAGAATATAAAAAATCAGTTTAAGGCCGTTTTTGAGGAGTTTGATATTGAATATGTTAAGACTGGA
GTTTTAACAAAACCAGCTATCGATACTCTGCTAAAATATATTGACAAGTATGATTTAAAAGTTATATGCGACCCGGTTCT
TGCATCTACAACAAAGTTTTCATTTGTTGATGAAAAGTTGATGGAAAAATATATTGAGCTTTTTAATAAGAGCTTTTTAA
TAACTCCTAACAAAGAAGAATATAAGAAAATCATGGAATTTATAAAAAATAACAATCTGATGATTAGAAATGATTTATAC
ATCTTAGCTACTGGCATTGATGACATTTTAATGAAAAATTTTAAACCTATAAAGACATTTAAAGGATTTAGAGTTGATAA
AGAAGTTCATGGAACTGGTTGTGTTTATTCAACAGCTATAACTGCTTTTTTATCTAAGGGCTATGATTTAGAAGAGGCAA
TTAAAGAGGCAAAGAGATTTGTTTTATCTTCAGTTATCTATGCCAAAAAATCAAAGTTTGGCTATAACTCTAACCCAACT
TATATCAATAAAGAAAAAGTGATAAAAAACCTTAGCTATGCCATATATTTATTAAAAAAGATGAACTTTACGTTAATCCC
AGAGGTTGGTAGTAATATAGCTGAGTCTCTACCATTTCCAAAAGATTTTAAGGATGTTGCTGCATTAACTGGAAGGATAA
TAAAAAATAAGCTTGGAGGTTTTTATATTGTTGGAGATATTGAGTTTGGAGCTTCTGAGCATATAGCAAAGATAATTTTA
TCTGCAAGCAAATTCAACCCAGAGATAAGGGCTTGCATGAATATAAAATATGATGGGGGGTTAATAAAGTTGTTGAAGGA
TAAATTTGCAGTTTCTTCATTTGATAGAAAAGAAGAGCCGCCAAATGTCTCAACTATGGAATGGGGAACAAAAATAGCTT
GTGAAAAATTTGGTGGAGTTCCAGACATTATTTATGATAGGGGAGGGGAAGGAAAAGAGCCGATGATTAGAGTTTTAGGT
AGAGATGCTATTGAAGTTGTTAAAAAAGTTGAAGTTATTCAAAAGATATATAACACATTAATGTAA

Upstream 100 bases:

>100_bases
CAAATTAAAGATGACTTAGAGAGAATTGAATTTTTAGAGGAGAATATAGAGAGGGTTATGAAGTGTTTAAGAGAGATAAA
TGTGAAATCATTATAAAATT

Downstream 100 bases:

>100_bases
ATATTTAAACATTTTAATTAAATTTTATAAAATTATAAAACTAAACCTTTTAGAAATCAAAAAATGGTGGACTGGGCGGG
ATTTGAACCCGCGGCCTCCG

Product: hypothetical protein

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MVILMVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEENIKNQFKAVFEEFDIEYVKTG
VLTKPAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEKLMEKYIELFNKSFLITPNKEEYKKIMEFIKNNNLMIRNDLY
ILATGIDDILMKNFKPIKTFKGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVLSSVIYAKKSKFGYNSNPT
YINKEKVIKNLSYAIYLLKKMNFTLIPEVGSNIAESLPFPKDFKDVAALTGRIIKNKLGGFYIVGDIEFGASEHIAKIIL
SASKFNPEIRACMNIKYDGGLIKLLKDKFAVSSFDRKEEPPNVSTMEWGTKIACEKFGGVPDIIYDRGGEGKEPMIRVLG
RDAIEVVKKVEVIQKIYNTLM

Sequences:

>Translated_421_residues
MVILMVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEENIKNQFKAVFEEFDIEYVKTG
VLTKPAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEKLMEKYIELFNKSFLITPNKEEYKKIMEFIKNNNLMIRNDLY
ILATGIDDILMKNFKPIKTFKGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVLSSVIYAKKSKFGYNSNPT
YINKEKVIKNLSYAIYLLKKMNFTLIPEVGSNIAESLPFPKDFKDVAALTGRIIKNKLGGFYIVGDIEFGASEHIAKIIL
SASKFNPEIRACMNIKYDGGLIKLLKDKFAVSSFDRKEEPPNVSTMEWGTKIACEKFGGVPDIIYDRGGEGKEPMIRVLG
RDAIEVVKKVEVIQKIYNTLM
>Mature_421_residues
MVILMVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEENIKNQFKAVFEEFDIEYVKTG
VLTKPAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEKLMEKYIELFNKSFLITPNKEEYKKIMEFIKNNNLMIRNDLY
ILATGIDDILMKNFKPIKTFKGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVLSSVIYAKKSKFGYNSNPT
YINKEKVIKNLSYAIYLLKKMNFTLIPEVGSNIAESLPFPKDFKDVAALTGRIIKNKLGGFYIVGDIEFGASEHIAKIIL
SASKFNPEIRACMNIKYDGGLIKLLKDKFAVSSFDRKEEPPNVSTMEWGTKIACEKFGGVPDIIYDRGGEGKEPMIRVLG
RDAIEVVKKVEVIQKIYNTLM

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=254, Percent_Identity=25.1968503937008, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325378, Length=252, Percent_Identity=25, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324997, Length=148, Percent_Identity=31.0810810810811, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324517, Length=171, Percent_Identity=30.4093567251462, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y236_METJA (Q57688)

Other databases:

- EMBL:   L77117
- PIR:   E64329
- RefSeq:   NP_247207.1
- PDB:   2PHP
- PDBsum:   2PHP
- ProteinModelPortal:   Q57688
- SMR:   Q57688
- GeneID:   1451089
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0236
- NMPDR:   fig|243232.1.peg.244
- TIGR:   MJ0236
- HOGENOM:   HBG476673
- OMA:   GDWGKEP
- ProtClustDB:   CLSK876123
- BioCyc:   MJAN243232:MJ_0236-MONOMER
- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293
- Gene3D:   G3DSA:3.40.225.10

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin

EC number: 2.7.4.7

Molecular weight: Translated: 47513; Mature: 47513

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILMVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEEN
CHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEECCHHHHHHCCCCCCEEEEECCCCHHH
IKNQFKAVFEEFDIEYVKTGVLTKPAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEKL
HHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECHHHHCCHHHHHHHHHH
MEKYIELFNKSFLITPNKEEYKKIMEFIKNNNLMIRNDLYILATGIDDILMKNFKPIKTF
HHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCEEEECCEEEEEECHHHHHHCCCCHHHHH
KGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVLSSVIYAKKSKFGYNSNPT
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YINKEKVIKNLSYAIYLLKKMNFTLIPEVGSNIAESLPFPKDFKDVAALTGRIIKNKLGG
EECHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
FYIVGDIEFGASEHIAKIILSASKFNPEIRACMNIKYDGGLIKLLKDKFAVSSFDRKEEP
EEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCC
PNVSTMEWGTKIACEKFGGVPDIIYDRGGEGKEPMIRVLGRDAIEVVKKVEVIQKIYNTL
CCCCCHHCCCHHHHHHHCCCCCHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHC
M
C
>Mature Secondary Structure
MVILMVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEEN
CHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEECCHHHHHHCCCCCCEEEEECCCCHHH
IKNQFKAVFEEFDIEYVKTGVLTKPAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEKL
HHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECHHHHCCHHHHHHHHHH
MEKYIELFNKSFLITPNKEEYKKIMEFIKNNNLMIRNDLYILATGIDDILMKNFKPIKTF
HHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCEEEECCEEEEEECHHHHHHCCCCHHHHH
KGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVLSSVIYAKKSKFGYNSNPT
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YINKEKVIKNLSYAIYLLKKMNFTLIPEVGSNIAESLPFPKDFKDVAALTGRIIKNKLGG
EECHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
FYIVGDIEFGASEHIAKIILSASKFNPEIRACMNIKYDGGLIKLLKDKFAVSSFDRKEEP
EEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCC
PNVSTMEWGTKIACEKFGGVPDIIYDRGGEGKEPMIRVLGRDAIEVVKKVEVIQKIYNTL
CCCCCHHCCCHHHHHHHCCCCCHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHC
M
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087