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The map label for this gene is amtB [C]

Identifier: 15668229

GI number: 15668229

Start: 57588

End: 58763

Strand: Reverse

Name: amtB [C]

Synonym: MJ0058

Alternate gene names: 15668229

Gene position: 58763-57588 (Counterclockwise)

Preceding gene: 15668236

Following gene: 15668228

Centisome position: 3.53

GC content: 37.59

Gene sequence:

>1176_bases
GTGTTTGAGGTGAAACATATGGATGGTATTGATGTTTTCTTTTTTATGTGGGCAGCATCGTTAATATTTTTCATGAAGGC
AGGGTTTATTGCGTTGGAAATAGGGCAGTTTAGGGCTAAAAACGTCTCATATCATTGTGTTTTAAAGTTGTTGGATTTAG
CTGCAGTGTTTATCGCTTATTTGTTCATTGGTTATGGTATCTCTTACGGATTTGAAAATATAATGCCCTTAATAACAGGA
ACTTTTGATGCTGATTTGGGAGCTTGGTGGATGAAGATGGTTATGTTTGCCGCTGCTGCAGTTACAATTATAACAGGAGG
AGTCGCTGAAAGAATTAAAATCTTACCTTACTTTATAGGGGCTTTGATTGTTGGAGGTATTTTGTATCCAATTGTTGAAC
ATTTAGTTTGGGGAGGAGGTTTTGCTAATTTAGGAATAAACTTCCACGACTATGCTGGAAGTGGGGCAGTTCATTTATTT
GGTGGTTTAGTTGGTTTAATGGCTGCCTATGTTTTAGGGCCAAGAATTGATAAATATATAAATGGAAAACCACAGGCAAT
TCCAGGGCATAACATTCCAATAGCTGTTTTAGGAGCTTTTATTTTGGCATTTGGATGGTACGGATTCAACATTGGAAGTG
CTTCTGGCATAGCTAATGGAGTAGAGTTGGCAAGCGTAGCTATGGCAACAACAATGGCTTTAGCTGGAGGAATTATAGGG
GGAGCATTAAGCTCAAGAAACGACCCTCTTTACACAGCAAACGGTATGTGTGCTGGTTTAGTAGCTGTTTGTAGTGGAGT
TGATTTATTCACTCCAATTGGAGCGTTTATAGTTGGTTTATTAGCAGGGATTCAGCAGCCATTTACATACAAGTTTATTG
AAGAGAAATTAAAGATTGATGACGTCTGTGCTATAGGGCCAGTTCATGCTATGAGTGGTTTGATTGGAGTTATCTGTGCA
GGAATTCCATTCTTATTAAAAGCTGATGCAGTGTCTAAAGTTTCAATTACTGGGCAAATAATTGGGGCTATTGTTATTGC
TTTAATTGCAATCGTTGGAGGATTAATTATTTATAAAGGGTTGGATTTAACAATTGGCTTAAGAGTCAGTGAAGAAGCAG
AGAAAGTTGGTTTAGATACTGCAATATTGCAAACAACTGCATATTCAGAAGAATAA

Upstream 100 bases:

>100_bases
TAATAGGAAGGAAAATTCTTTCCTTAGTAAGTTATATATTTAAGCTTTTCCATTAGGGGAATTAGGAAGATGGGTAGTAG
GTAAGATAATTCCTATAAAA

Downstream 100 bases:

>100_bases
ACTTAATAATTTTTTATTACGCATATTCCTTTTTTTAATCTCCAATTATTTTCCTCGATAAAATATTTATATGATTTTTA
GATTTTAAATATTCAGCCAA

Product: ammonium transporter AmtB

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFEVKHMDGIDVFFFMWAASLIFFMKAGFIALEIGQFRAKNVSYHCVLKLLDLAAVFIAYLFIGYGISYGFENIMPLITG
TFDADLGAWWMKMVMFAAAAVTIITGGVAERIKILPYFIGALIVGGILYPIVEHLVWGGGFANLGINFHDYAGSGAVHLF
GGLVGLMAAYVLGPRIDKYINGKPQAIPGHNIPIAVLGAFILAFGWYGFNIGSASGIANGVELASVAMATTMALAGGIIG
GALSSRNDPLYTANGMCAGLVAVCSGVDLFTPIGAFIVGLLAGIQQPFTYKFIEEKLKIDDVCAIGPVHAMSGLIGVICA
GIPFLLKADAVSKVSITGQIIGAIVIALIAIVGGLIIYKGLDLTIGLRVSEEAEKVGLDTAILQTTAYSEE

Sequences:

>Translated_391_residues
MFEVKHMDGIDVFFFMWAASLIFFMKAGFIALEIGQFRAKNVSYHCVLKLLDLAAVFIAYLFIGYGISYGFENIMPLITG
TFDADLGAWWMKMVMFAAAAVTIITGGVAERIKILPYFIGALIVGGILYPIVEHLVWGGGFANLGINFHDYAGSGAVHLF
GGLVGLMAAYVLGPRIDKYINGKPQAIPGHNIPIAVLGAFILAFGWYGFNIGSASGIANGVELASVAMATTMALAGGIIG
GALSSRNDPLYTANGMCAGLVAVCSGVDLFTPIGAFIVGLLAGIQQPFTYKFIEEKLKIDDVCAIGPVHAMSGLIGVICA
GIPFLLKADAVSKVSITGQIIGAIVIALIAIVGGLIIYKGLDLTIGLRVSEEAEKVGLDTAILQTTAYSEE
>Mature_391_residues
MFEVKHMDGIDVFFFMWAASLIFFMKAGFIALEIGQFRAKNVSYHCVLKLLDLAAVFIAYLFIGYGISYGFENIMPLITG
TFDADLGAWWMKMVMFAAAAVTIITGGVAERIKILPYFIGALIVGGILYPIVEHLVWGGGFANLGINFHDYAGSGAVHLF
GGLVGLMAAYVLGPRIDKYINGKPQAIPGHNIPIAVLGAFILAFGWYGFNIGSASGIANGVELASVAMATTMALAGGIIG
GALSSRNDPLYTANGMCAGLVAVCSGVDLFTPIGAFIVGLLAGIQQPFTYKFIEEKLKIDDVCAIGPVHAMSGLIGVICA
GIPFLLKADAVSKVSITGQIIGAIVIALIAIVGGLIIYKGLDLTIGLRVSEEAEKVGLDTAILQTTAYSEE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family

Homologues:

Organism=Homo sapiens, GI224548945, Length=190, Percent_Identity=32.6315789473684, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=32.6034063260341, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=426, Percent_Identity=32.6291079812207, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550182, Length=426, Percent_Identity=30.9859154929577, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=424, Percent_Identity=28.0660377358491, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=419, Percent_Identity=28.4009546539379, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=28.2710280373832, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=26.9320843091335, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=320, Percent_Identity=28.4375, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161078314, Length=403, Percent_Identity=28.0397022332506, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y058_METJA (Q60366)

Other databases:

- EMBL:   L77117
- PIR:   B64307
- RefSeq:   NP_247022.1
- ProteinModelPortal:   Q60366
- GeneID:   1450897
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0058
- NMPDR:   fig|243232.1.peg.59
- TIGR:   MJ0058
- HOGENOM:   HBG677626
- OMA:   QTTAYSE
- ProtClustDB:   CLSK796258
- BioCyc:   MJAN243232:MJ_0058-MONOMER
- InterPro:   IPR001905
- InterPro:   IPR018047
- PANTHER:   PTHR11730
- TIGRFAMs:   TIGR00836

Pfam domain/function: PF00909 Ammonium_transp; SSF111352 RH_like_transpt

EC number: NA

Molecular weight: Translated: 41153; Mature: 41153

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01219 AMMONIUM_TRANSP; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xaf74348)-; HASH(0xbcf635c)-; HASH(0xbcb85c0)-; HASH(0xbd615a4)-; HASH(0xbd7529c)-; HASH(0xbd73450)-; HASH(0xbcb0934)-; HASH(0xbcdd464)-; HASH(0xbcdf3a8)-; HASH(0xbcecfa4)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEEEECHHHHHHCCHHHHHHHHCCCCH
>Mature Secondary Structure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CCEEEEEEECHHHHHHCCHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087