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The map label for this gene is mfnA

Identifier: 15668221

GI number: 15668221

Start: 51047

End: 52237

Strand: Reverse

Name: mfnA

Synonym: MJ0050

Alternate gene names: 15668221

Gene position: 52237-51047 (Counterclockwise)

Preceding gene: 15668223

Following gene: 15668220

Centisome position: 3.14

GC content: 32.83

Gene sequence:

>1191_bases
ATGAGAAACATGCAGGAAAAAGGTGTTAGTGAGAAAGAAATTTTAGAGGAATTGAAAAAATATAGGAGTTTGGATTTAAA
GTATGAAGATGGAAATATTTTCGGTTCAATGTGTTCCAATGTATTACCAATAACAAGAAAAATTGTAGATATCTTCTTAG
AAACAAACTTGGGAGACCCTGGACTATTTAAAGGGACTAAATTGTTAGAAGAAAAAGCTGTGGCTTTATTGGGTTCTTTG
TTAAATAACAAAGATGCCTATGGACATATAGTTAGTGGAGGGACTGAAGCCAACTTAATGGCTTTAAGATGCATAAAAAA
TATATGGAGGGAAAAAAGGAGAAAGGGCTTATCAAAAAATGAACATCCAAAGATTATCGTTCCAATAACTGCCCATTTCT
CATTCGAAAAAGGAAGAGAAATGATGGACTTAGAGTATATCTATGCCCCAATTAAAGAAGATTATACAATAGATGAGAAA
TTCGTTAAAGATGCCGTAGAGGATTATGATGTAGATGGCATTATAGGAATTGCTGGAACAACAGAGCTTGGAACTATTGA
CAACATAGAGGAGCTAAGTAAAATAGCAAAAGAAAACAACATTTATATCCATGTAGATGCGGCATTTGGAGGCTTAGTAA
TTCCATTTTTAGATGATAAATATAAGAAAAAAGGAGTAAATTATAAATTTGACTTTTCTTTGGGAGTTGATTCTATAACC
ATAGACCCCCATAAAATGGGGCACTGCCCAATCCCAAGTGGAGGGATTCTATTTAAAGATATAGGTTATAAAAGATATTT
GGATGTTGATGCCCCTTATTTAACTGAAACAAGACAGGCAACAATCTTAGGAACAAGGGTTGGATTCGGAGGAGCCTGCA
CTTATGCAGTTTTAAGATATTTAGGTAGAGAGGGACAGCGAAAAATTGTTAATGAATGTATGGAAAACACCCTTTATCTT
TACAAAAAATTGAAGGAAAATAATTTTAAACCAGTCATTGAACCAATATTAAATATTGTTGCAATTGAAGATGAAGATTA
TAAAGAAGTCTGCAAAAAACTTAGAGATAGAGGCATTTACGTTTCAGTTTGCAATTGTGTTAAAGCTTTGAGAATCGTTG
TTATGCCACATATTAAGAGGGAGCATATAGATAATTTTATCGAAATATTGAATAGTATTAAAAGGGATTGA

Upstream 100 bases:

>100_bases
TCAAATAAATGTTTAGTATATTGTATTATATTTATACCTAAGAACGCATTAAATACTTATAAATTTAATCATGATGTTTA
TCAACTAAAAAAATTAAAGG

Downstream 100 bases:

>100_bases
TTGAAAAGATTGAAATTGAAAAAGTATTTATATCTTAATAAAATAATAAATATATTAATAATTTATGTATATTAATAATT
TATGCTTTTAGTTATTATAA

Product: L-tyrosine decarboxylase

Products: NA

Alternate protein names: TDC

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDPGLFKGTKLLEEKAVALLGSL
LNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKNEHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEK
FVKDAVEDYDVDGIIGIAGTTELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNYKFDFSLGVDSIT
IDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLTETRQATILGTRVGFGGACTYAVLRYLGREGQRKIVNECMENTLYL
YKKLKENNFKPVIEPILNIVAIEDEDYKEVCKKLRDRGIYVSVCNCVKALRIVVMPHIKREHIDNFIEILNSIKRD

Sequences:

>Translated_396_residues
MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDPGLFKGTKLLEEKAVALLGSL
LNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKNEHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEK
FVKDAVEDYDVDGIIGIAGTTELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNYKFDFSLGVDSIT
IDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLTETRQATILGTRVGFGGACTYAVLRYLGREGQRKIVNECMENTLYL
YKKLKENNFKPVIEPILNIVAIEDEDYKEVCKKLRDRGIYVSVCNCVKALRIVVMPHIKREHIDNFIEILNSIKRD
>Mature_396_residues
MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDPGLFKGTKLLEEKAVALLGSL
LNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKNEHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEK
FVKDAVEDYDVDGIIGIAGTTELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNYKFDFSLGVDSIT
IDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLTETRQATILGTRVGFGGACTYAVLRYLGREGQRKIVNECMENTLYL
YKKLKENNFKPVIEPILNIVAIEDEDYKEVCKKLRDRGIYVSVCNCVKALRIVVMPHIKREHIDNFIEILNSIKRD

Specific function: Specifically catalyzes the decarboxylation of L-tyrosine to produce tyramine

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Archaeal L-tyrosine decarboxylase subfamily

Homologues:

Organism=Homo sapiens, GI31982936, Length=361, Percent_Identity=28.2548476454294, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17543922, Length=397, Percent_Identity=27.9596977329975, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25148342, Length=331, Percent_Identity=26.5861027190332, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17557272, Length=271, Percent_Identity=29.1512915129151, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320500, Length=408, Percent_Identity=20.343137254902, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323905, Length=355, Percent_Identity=23.6619718309859, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355963, Length=335, Percent_Identity=26.865671641791, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24654344, Length=335, Percent_Identity=26.865671641791, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): MFNA_METJA (Q60358)

Other databases:

- EMBL:   L77117
- PIR:   B64306
- RefSeq:   NP_247014.1
- PDB:   3F9T
- PDBsum:   3F9T
- ProteinModelPortal:   Q60358
- GeneID:   1450889
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0050
- NMPDR:   fig|243232.1.peg.51
- TIGR:   MJ0050
- HOGENOM:   HBG497871
- OMA:   ILGSMCT
- ProtClustDB:   PRK13520
- BioCyc:   MetaCyc:MONOMER-12228
- BioCyc:   MJAN243232:MJ_0050-MONOMER
- BRENDA:   4.1.1.25
- HAMAP:   MF_01610
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR020931
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11999
- TIGRFAMs:   TIGR03812

Pfam domain/function: PF00282 Pyridoxal_deC; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.1.25

Molecular weight: Translated: 45051; Mature: 45051

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00392 DDC_GAD_HDC_YDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDP
CCCCHHCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCC
GLFKGTKLLEEKAVALLGSLLNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKN
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEKFVKDAVEDYDVDGIIGIAGT
CCCEEEEEEEEECCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCC
TELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNYKFDFSLGVDSIT
CCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEECCCCHHHHCCCCEEEEEEECCCEEE
IDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLTETRQATILGTRVGFGGACTYAVLRY
ECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
LGREGQRKIVNECMENTLYLYKKLKENNFKPVIEPILNIVAIEDEDYKEVCKKLRDRGIY
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHCCCE
VSVCNCVKALRIVVMPHIKREHIDNFIEILNSIKRD
EEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDP
CCCCHHCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCC
GLFKGTKLLEEKAVALLGSLLNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKN
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEKFVKDAVEDYDVDGIIGIAGT
CCCEEEEEEEEECCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCC
TELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNYKFDFSLGVDSIT
CCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEECCCCHHHHCCCCEEEEEEECCCEEE
IDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLTETRQATILGTRVGFGGACTYAVLRY
ECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
LGREGQRKIVNECMENTLYLYKKLKENNFKPVIEPILNIVAIEDEDYKEVCKKLRDRGIY
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHCCCE
VSVCNCVKALRIVVMPHIKREHIDNFIEILNSIKRD
EEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087