| Definition | Methanocaldococcus jannaschii DSM 2661 chromosome, complete genome. |
|---|---|
| Accession | NC_000909 |
| Length | 1,664,970 |
Click here to switch to the map view.
The map label for this gene is sdhA [C]
Identifier: 15668203
GI number: 15668203
Start: 33629
End: 35248
Strand: Direct
Name: sdhA [C]
Synonym: MJ0033
Alternate gene names: 15668203
Gene position: 33629-35248 (Clockwise)
Preceding gene: 15668202
Following gene: 15668206
Centisome position: 2.02
GC content: 34.69
Gene sequence:
>1620_bases ATGAAAACTGATATCTTAATTATAGGCGGAGGAGGAGCTGCTGCAAGGGCGGCAATAGAATGCAGAGATAAGAATGTCAT AATAGCTGTTAAGGGATTGTTTGGAAAGAGTGGATGCACAGTTATGGCTGAAGGAGGTTATAACGCAGTATTTAATCCAA AAGATAGCTTTAAGAAGCATTTTTATGACACAGTAAAAGGAGGGGGATTTATAAACAATCCAAAGCTGGTAGAGATTTTA GTTAAAAATGCTCCTAAAGAGCTTTTGAACTTAGAAAGGTTTGGAGCTTTGTTTGATAGGACTGAAGATGGCTTTATAGC TCAAAGACCATTTGGAGGGCAGAGTTTTAATAGAACTTGCTACTGTGGAGATAGAACAGGACATGAAATAATGAGAGGTT TAATGGAATATATCTCAAAATTTGAAAGGATTAAGATTTTAGAGGAGGTTATGGCAATAAAGTTGATTGTTAAAGATAAC AGATGCTATGGAGCGATATTTTTAGATTTAAAGACTGGGAATATATTTCCAATATTTGCTAAAGCAACTATATTGGCAAC TGGAGGAGCTGGGCAACTATATCCAATAACATCCAATCCAATACAAAAGACTGGAGATGGTTTTGCTATAGCTTATAATG AAGGAGCTGAACTTATAGACATGGAGATGGTTCAATTCCATCCAACTGGAATGGTTGGGACTGGAATTTTAGTTACAGAG GCCGTGAGAGGAGAAGGAGGAATTTTATACAATAAATATAAAGAGAGATTCATGGTAAGATACGACAAGGAGAGAATGGA GCTATCGACAAGGGATGTTGTTGCAAGAGCTATATATAAAGAGATTCAAGAAGGTAGAGGAGTTAATGGAGGAGTTTATT TAGACGTTTCTCATTTGCCTAATGAGGTTATTGAAAAGAAGTTAGAAACTATGTTAAAGCAATTTTTGAGAGTAGGAATT GATATTAGAAAGGAGCCGATGATTGTTTCTCCGACTGCTCATCATTTTATGGGAGGTTTAAAGATTAATGAAAGATGTGA AACCAATATAATTGGATTATTTGCATGTGGAGAGGTTACAGGGGGAGTTCATGGAGCTAATAGATTAGGAGGGAACGCTT TAGCAGATACCCAGGTCTTTGGAGCTATTGCTGGAAAATCTGCCAAAGAGTTTGTTGAAAATCATGATTTTAATAATATT GATGCTGAAGAAGATGTTGCCAAAATATTGGAGGAGATAAATAGCTTGAAAGGAGATTTAAACGTCTATAATTTAATTGA AGATTTGAGAAAGGTTATGTGGGATTATGTATCCATTATTAGAAATGAGGATGGATTAAAAAAAGCCTTAGAAAAAATTG ATGAAATTGAGAGAAATATAGATAATGTCAAAGTTAATGGAATTATTGACTTGCAGAACTACTTTGAATTGAAAAACATG GTTGTTGTTGCTAAGTTGGTTACAAAATCTGCTTTATATAGAAAGGAGAGTAGAGGAGCACATTATAGGGAAGACTTTCC AGAAACAAAGGAAGAGTGGAGAGGAAATATTATTATAAAAGGAAAAAAGATGTGGTTTGAAAAGTTGGATTATAGTGTCT TTCAAAACTTCTTGGAATAA
Upstream 100 bases:
>100_bases ATGCCAATTTTTTCATTTAGGATTATTTTTTTCATCAAAGCTATTTTGATTTATAAGTTTTAAATATCTGATGAGGGTAT CCTATTTTTGGTGATAAAAA
Downstream 100 bases:
>100_bases CTGAGGTATAAATGAACGCCTTCCGAAGGAAGGGCGTTCAAACCTTCCTTAGTAAATTTTTATTAATTTTGAAAGACACT ACGATGTTGATGAATATCTA
Product: succinate dehydrogenase flavoprotein subunit
Products: fumarate; reduced acceptor
Alternate protein names: NA
Number of amino acids: Translated: 539; Mature: 539
Protein sequence:
>539_residues MKTDILIIGGGGAAARAAIECRDKNVIIAVKGLFGKSGCTVMAEGGYNAVFNPKDSFKKHFYDTVKGGGFINNPKLVEIL VKNAPKELLNLERFGALFDRTEDGFIAQRPFGGQSFNRTCYCGDRTGHEIMRGLMEYISKFERIKILEEVMAIKLIVKDN RCYGAIFLDLKTGNIFPIFAKATILATGGAGQLYPITSNPIQKTGDGFAIAYNEGAELIDMEMVQFHPTGMVGTGILVTE AVRGEGGILYNKYKERFMVRYDKERMELSTRDVVARAIYKEIQEGRGVNGGVYLDVSHLPNEVIEKKLETMLKQFLRVGI DIRKEPMIVSPTAHHFMGGLKINERCETNIIGLFACGEVTGGVHGANRLGGNALADTQVFGAIAGKSAKEFVENHDFNNI DAEEDVAKILEEINSLKGDLNVYNLIEDLRKVMWDYVSIIRNEDGLKKALEKIDEIERNIDNVKVNGIIDLQNYFELKNM VVVAKLVTKSALYRKESRGAHYREDFPETKEEWRGNIIIKGKKMWFEKLDYSVFQNFLE
Sequences:
>Translated_539_residues MKTDILIIGGGGAAARAAIECRDKNVIIAVKGLFGKSGCTVMAEGGYNAVFNPKDSFKKHFYDTVKGGGFINNPKLVEIL VKNAPKELLNLERFGALFDRTEDGFIAQRPFGGQSFNRTCYCGDRTGHEIMRGLMEYISKFERIKILEEVMAIKLIVKDN RCYGAIFLDLKTGNIFPIFAKATILATGGAGQLYPITSNPIQKTGDGFAIAYNEGAELIDMEMVQFHPTGMVGTGILVTE AVRGEGGILYNKYKERFMVRYDKERMELSTRDVVARAIYKEIQEGRGVNGGVYLDVSHLPNEVIEKKLETMLKQFLRVGI DIRKEPMIVSPTAHHFMGGLKINERCETNIIGLFACGEVTGGVHGANRLGGNALADTQVFGAIAGKSAKEFVENHDFNNI DAEEDVAKILEEINSLKGDLNVYNLIEDLRKVMWDYVSIIRNEDGLKKALEKIDEIERNIDNVKVNGIIDLQNYFELKNM VVVAKLVTKSALYRKESRGAHYREDFPETKEEWRGNIIIKGKKMWFEKLDYSVFQNFLE >Mature_539_residues MKTDILIIGGGGAAARAAIECRDKNVIIAVKGLFGKSGCTVMAEGGYNAVFNPKDSFKKHFYDTVKGGGFINNPKLVEIL VKNAPKELLNLERFGALFDRTEDGFIAQRPFGGQSFNRTCYCGDRTGHEIMRGLMEYISKFERIKILEEVMAIKLIVKDN RCYGAIFLDLKTGNIFPIFAKATILATGGAGQLYPITSNPIQKTGDGFAIAYNEGAELIDMEMVQFHPTGMVGTGILVTE AVRGEGGILYNKYKERFMVRYDKERMELSTRDVVARAIYKEIQEGRGVNGGVYLDVSHLPNEVIEKKLETMLKQFLRVGI DIRKEPMIVSPTAHHFMGGLKINERCETNIIGLFACGEVTGGVHGANRLGGNALADTQVFGAIAGKSAKEFVENHDFNNI DAEEDVAKILEEINSLKGDLNVYNLIEDLRKVMWDYVSIIRNEDGLKKALEKIDEIERNIDNVKVNGIIDLQNYFELKNM VVVAKLVTKSALYRKESRGAHYREDFPETKEEWRGNIIIKGKKMWFEKLDYSVFQNFLE
Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]
COG id: COG1053
COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily
Homologues:
Organism=Homo sapiens, GI156416003, Length=527, Percent_Identity=35.8633776091082, Blast_Score=302, Evalue=4e-82, Organism=Escherichia coli, GI1790597, Length=494, Percent_Identity=36.4372469635627, Blast_Score=299, Evalue=3e-82, Organism=Escherichia coli, GI1786942, Length=521, Percent_Identity=38.0038387715931, Blast_Score=293, Evalue=1e-80, Organism=Escherichia coli, GI1788928, Length=524, Percent_Identity=33.969465648855, Blast_Score=283, Evalue=2e-77, Organism=Caenorhabditis elegans, GI17505833, Length=531, Percent_Identity=36.5348399246704, Blast_Score=305, Evalue=4e-83, Organism=Caenorhabditis elegans, GI17550100, Length=547, Percent_Identity=35.6489945155393, Blast_Score=300, Evalue=2e-81, Organism=Caenorhabditis elegans, GI71986328, Length=466, Percent_Identity=29.1845493562232, Blast_Score=120, Evalue=2e-27, Organism=Saccharomyces cerevisiae, GI6322701, Length=520, Percent_Identity=36.7307692307692, Blast_Score=306, Evalue=5e-84, Organism=Saccharomyces cerevisiae, GI6322416, Length=522, Percent_Identity=36.9731800766284, Blast_Score=303, Evalue=6e-83, Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=28.9855072463768, Blast_Score=114, Evalue=5e-26, Organism=Saccharomyces cerevisiae, GI6320788, Length=474, Percent_Identity=28.2700421940928, Blast_Score=112, Evalue=1e-25, Organism=Drosophila melanogaster, GI17137288, Length=528, Percent_Identity=35.4166666666667, Blast_Score=301, Evalue=9e-82, Organism=Drosophila melanogaster, GI24655642, Length=528, Percent_Identity=35.4166666666667, Blast_Score=301, Evalue=9e-82, Organism=Drosophila melanogaster, GI24655647, Length=528, Percent_Identity=35.4166666666667, Blast_Score=301, Evalue=9e-82, Organism=Drosophila melanogaster, GI24663005, Length=531, Percent_Identity=33.8983050847458, Blast_Score=278, Evalue=9e-75,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): Y033_METJA (Q60356)
Other databases:
- EMBL: L77117 - PIR: A64304 - RefSeq: NP_246996.1 - ProteinModelPortal: Q60356 - SMR: Q60356 - GeneID: 1450871 - GenomeReviews: L77117_GR - KEGG: mja:MJ_0033 - NMPDR: fig|243232.1.peg.33 - TIGR: MJ0033 - HOGENOM: HBG293998 - OMA: SGGMHGS - ProtClustDB: PRK06263 - BioCyc: MJAN243232:MJ_0033-MONOMER - InterPro: IPR003953 - InterPro: IPR013027 - InterPro: IPR015939 - InterPro: IPR004112 - Gene3D: G3DSA:1.20.58.100 - PRINTS: PR00368
Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C
EC number: 1.3.99.1
Molecular weight: Translated: 60399; Mature: 60399
Theoretical pI: Translated: 7.00; Mature: 7.00
Prosite motif: NA
Important sites: ACT_SITE 227-227 ACT_SITE 243-243
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKTDILIIGGGGAAARAAIECRDKNVIIAVKGLFGKSGCTVMAEGGYNAVFNPKDSFKKH CCCCEEEEECCCHHHHHEEEECCCCEEEEEEECCCCCCCEEEECCCCCCEECCHHHHHHH FYDTVKGGGFINNPKLVEILVKNAPKELLNLERFGALFDRTEDGFIAQRPFGGQSFNRTC HHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEE YCGDRTGHEIMRGLMEYISKFERIKILEEVMAIKLIVKDNRCYGAIFLDLKTGNIFPIFA EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEEEECCCEEEEEE KATILATGGAGQLYPITSNPIQKTGDGFAIAYNEGAELIDMEMVQFHPTGMVGTGILVTE EEEEEEECCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEHHHEECCCCCCCCCEEEEE AVRGEGGILYNKYKERFMVRYDKERMELSTRDVVARAIYKEIQEGRGVNGGVYLDVSHLP ECCCCCCEEEHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCC NEVIEKKLETMLKQFLRVGIDIRKEPMIVSPTAHHFMGGLKINERCETNIIGLFACGEVT HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHCCCEECCCCCCCEEEEEEECCCC GGVHGANRLGGNALADTQVFGAIAGKSAKEFVENHDFNNIDAEEDVAKILEEINSLKGDL CCCCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC NVYNLIEDLRKVMWDYVSIIRNEDGLKKALEKIDEIERNIDNVKVNGIIDLQNYFELKNM HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHH VVVAKLVTKSALYRKESRGAHYREDFPETKEEWRGNIIIKGKKMWFEKLDYSVFQNFLE HHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHCCCEEEECHHHHHHHHHHHHHHHHHC >Mature Secondary Structure MKTDILIIGGGGAAARAAIECRDKNVIIAVKGLFGKSGCTVMAEGGYNAVFNPKDSFKKH CCCCEEEEECCCHHHHHEEEECCCCEEEEEEECCCCCCCEEEECCCCCCEECCHHHHHHH FYDTVKGGGFINNPKLVEILVKNAPKELLNLERFGALFDRTEDGFIAQRPFGGQSFNRTC HHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEE YCGDRTGHEIMRGLMEYISKFERIKILEEVMAIKLIVKDNRCYGAIFLDLKTGNIFPIFA EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEEEECCCEEEEEE KATILATGGAGQLYPITSNPIQKTGDGFAIAYNEGAELIDMEMVQFHPTGMVGTGILVTE EEEEEEECCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEHHHEECCCCCCCCCEEEEE AVRGEGGILYNKYKERFMVRYDKERMELSTRDVVARAIYKEIQEGRGVNGGVYLDVSHLP ECCCCCCEEEHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCC NEVIEKKLETMLKQFLRVGIDIRKEPMIVSPTAHHFMGGLKINERCETNIIGLFACGEVT HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHCCCEECCCCCCCEEEEEEECCCC GGVHGANRLGGNALADTQVFGAIAGKSAKEFVENHDFNNIDAEEDVAKILEEINSLKGDL CCCCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC NVYNLIEDLRKVMWDYVSIIRNEDGLKKALEKIDEIERNIDNVKVNGIIDLQNYFELKNM HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHH VVVAKLVTKSALYRKESRGAHYREDFPETKEEWRGNIIIKGKKMWFEKLDYSVFQNFLE HHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHCCCEEEECHHHHHHHHHHHHHHHHHC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]
Metal ions: Anions; Diphosphate; Fe [C]
Kcat value (1/min): 10000-13000 [C]
Specific activity: 509
Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]
Substrates: succinate; acceptor
Specific reaction: succinate + acceptor = fumarate + reduced acceptor
General reaction: Redox reaction [C]
Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8688087