Click here to switch to the map view.

The map label for this gene is frhB

Identifier: 15668202

GI number: 15668202

Start: 32511

End: 33374

Strand: Direct

Name: frhB

Synonym: MJ0032

Alternate gene names: 15668202

Gene position: 32511-33374 (Clockwise)

Preceding gene: 15668201

Following gene: 15668203

Centisome position: 1.95

GC content: 37.04

Gene sequence:

>864_bases
TTGAGGTGGAATAGGATGAATCCTTTTGGTAGCTATAAAAAAGTTGTCTCAGCAAGGAGTACATTAAAAGAAGTTTTAAA
GAAGGCTCAGGATGGGGGAATCGTTTCAACAGCTTTTATTTATGGATTAGAAAATAATTTATTAGATGGAGTTATAGTTG
CAGACAACGCTGGGGAGTTTAAGGCAGTTCCTAAAGTAGCTACAACACCAGAAGAAGTTTTAGAGGCAGCTGGAACAAAA
TATACAGTCTGCCCAAACATTTCAGTATTAAAGAGTGCTGTTAGAGAATATGGCTGTGAAAAAATTGGAGTTGTTGGAAC
ACCTTGCCAAGTTAGAGCTGTAAGAAAGTTGATGAAATATCCAGTAGGATTTAGACACGTTCCAGATAAGATTGCTTTAA
TAATCGGAATCTTCTGTATGGAGAACTTCCCATACTACGGATTGAAGTTGATTGTTGAAGAACACTGTGGAGTTAAGATG
GAGGATGTCGTCAAGATGGATATTGGAAAGGGTAAGTTCTGGGTCTATACAAGATGGGGAGAGACAAAAGCGATTAAATT
AAAAGAAACTCATCCTTATGAACAAATCGCTTGCCATGTTTGTACAGATTATACCGCAGAGTTGGCAGATATTTCAACAG
GTTCAGTTGGAAGCCCAGACGGATGGAGCACTGTATTTATAAGAACTGCTAAGGGAGAAGAAATATTTAACAAAATGGTT
GAAGATGGGTATTTAGAAGTTAAACCAATAGAAGAAGTTAAACCAGGATTAGGATTAGTTGAGAAATTAGCACTGCAAAA
GAAAGAGAAAAACATGAAAGAAATCGAACATAGAAAAGAGCTCGGACTACCTATTCCATACTAA

Upstream 100 bases:

>100_bases
GAACTCTGTATTAAGTGTGGAGCTTGCTCTGTCCAGTGTCCAAGAATTAGATTCCCAGAGTTAATTGAGAAAATAGAATA
AAATAATATAATATAATTTT

Downstream 100 bases:

>100_bases
GAGGCACTGCCGAGCGTAGCGAGGCAGTGCATCCGTTTTGATGAACCTTTTACTAAAAGGTTCATGTGAAGGAAATAGAG
CATAGGAAAGAATTAGGATT

Product: coenzyme F420-reducing hydrogenase subunit beta

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta; FRH

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRWNRMNPFGSYKKVVSARSTLKEVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEFKAVPKVATTPEEVLEAAGTK
YTVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKYPVGFRHVPDKIALIIGIFCMENFPYYGLKLIVEEHCGVKM
EDVVKMDIGKGKFWVYTRWGETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFIRTAKGEEIFNKMV
EDGYLEVKPIEEVKPGLGLVEKLALQKKEKNMKEIEHRKELGLPIPY

Sequences:

>Translated_287_residues
MRWNRMNPFGSYKKVVSARSTLKEVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEFKAVPKVATTPEEVLEAAGTK
YTVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKYPVGFRHVPDKIALIIGIFCMENFPYYGLKLIVEEHCGVKM
EDVVKMDIGKGKFWVYTRWGETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFIRTAKGEEIFNKMV
EDGYLEVKPIEEVKPGLGLVEKLALQKKEKNMKEIEHRKELGLPIPY
>Mature_287_residues
MRWNRMNPFGSYKKVVSARSTLKEVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEFKAVPKVATTPEEVLEAAGTK
YTVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKYPVGFRHVPDKIALIIGIFCMENFPYYGLKLIVEEHCGVKM
EDVVKMDIGKGKFWVYTRWGETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFIRTAKGEEIFNKMV
EDGYLEVKPIEEVKPGLGLVEKLALQKKEKNMKEIEHRKELGLPIPY

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the frhB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRHB_METJA (Q60341)

Other databases:

- EMBL:   L77117
- PIR:   H64303
- RefSeq:   NP_246995.1
- GeneID:   1450870
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0032
- NMPDR:   fig|243232.1.peg.32
- TIGR:   MJ0032
- HOGENOM:   HBG726753
- OMA:   KSAVREY
- ProtClustDB:   PRK09325
- BioCyc:   MJAN243232:MJ_0032-MONOMER
- BRENDA:   1.12.98.1
- InterPro:   IPR007516
- InterPro:   IPR017679
- InterPro:   IPR007525
- TIGRFAMs:   TIGR03289

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N

EC number: =1.12.98.1

Molecular weight: Translated: 32055; Mature: 32055

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWNRMNPFGSYKKVVSARSTLKEVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHEEECCCCCCCCEEEECCCCCC
KAVPKVATTPEEVLEAAGTKYTVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKY
CCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
PVGFRHVPDKIALIIGIFCMENFPYYGLKLIVEEHCGVKMEDVVKMDIGKGKFWVYTRWG
CCCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCHHHHHEEECCCCEEEEEEECC
ETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFIRTAKGEEIFNKMV
CCEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
EDGYLEVKPIEEVKPGLGLVEKLALQKKEKNMKEIEHRKELGLPIPY
HCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRWNRMNPFGSYKKVVSARSTLKEVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHEEECCCCCCCCEEEECCCCCC
KAVPKVATTPEEVLEAAGTKYTVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKY
CCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
PVGFRHVPDKIALIIGIFCMENFPYYGLKLIVEEHCGVKMEDVVKMDIGKGKFWVYTRWG
CCCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCHHHHHEEECCCCEEEEEEECC
ETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFIRTAKGEEIFNKMV
CCEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
EDGYLEVKPIEEVKPGLGLVEKLALQKKEKNMKEIEHRKELGLPIPY
HCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087