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The map label for this gene is thiL

Identifier: 15668199

GI number: 15668199

Start: 28514

End: 29473

Strand: Reverse

Name: thiL

Synonym: MJ0028

Alternate gene names: 15668199

Gene position: 29473-28514 (Counterclockwise)

Preceding gene: 15668204

Following gene: 15668198

Centisome position: 1.77

GC content: 32.08

Gene sequence:

>960_bases
ATGGACGAGATGAAAGTAATTGAAATAATCAAAAAAACTCTAAAGTTTTCTAATGAAAATATTGTAAAAGGCATTGATGA
TGACTGTGCAATTATAAAAATTGATGAAAATTTTTATTTAGTTGCTACAACAGACATGATGGTTAAAAAAGCCCATATCC
CTTCTATATTATCCCCATATGAAATTGGAGGGAGAATTTTAACCGCCAATGTTTCAGATATTGCATCTATGGGAGCCAAG
CCATTGGCATTTTTAGTATCGATATCCCTATCTAAGGAAGAAGCAAATGAGAAGTTTATTAAAGAGCTTTATTCTGGCTT
AGATGATTTTTCTAAGCTTTATGACTGCCCAGTGGTTGGTGGGGATACAAATAGGGGAGATGAGCTCATATTATCAGGAA
CTGCCTTTGGAATAACTGACAATCCTATATATAGGAGAGGGAAAGTTGGGGATGATATCTGTGTAACTAATGATTTAGGT
AGGGTTTATTGTGCTTTAACTCTATATTATATGCTTAAAGAGAACAAAATTAGCTACAAAGAGTTTGAAAGACTCTGCCA
GAAATATCCAAAGATTATTGAAAAATTAAGAAAACCTATTGCAAGGATTAAAGAAGGGCTATTAATGAATAAACTCATAA
ATGGTTGTTGTGACATCTCAGACGGTTTGGGAAAGGAAATTACTTATTTCAAAAATTTTGAGATATACAGTGATAGGATT
TTTAAGCTTATTCCAGAAGATGTCATTGAATTTTGTGATGCCTTTAATTTAAACCCCATAAAAGTTGCTCTAAATAGTGG
AGAGGAGTTTGAGCTTTTATTCACAACATCTAAATTTAATAAAGTGAAAGATTCACTAAAAGGCTATTCAAAGATTTATA
AAATCGGTAAAATTATAGAAGATGGGCAGTTTATTGATGGAGAGGAATTTTATGGTGGAGGATACATTCACAAATGGTAA

Upstream 100 bases:

>100_bases
GAGTAGCAAGCCGGCTGATGCCACTTTTCCTTTTTTTATTTATCAACTAATAAAGAAAAGTCATAAAAAACTTCTTTTAT
TCTAATTAGAGGGGCTAAAA

Downstream 100 bases:

>100_bases
ATTGAGTAAAAAAGAAAAAATTTTGTTTTTGATAAAGTTTTATATTATATTTTTAGTAGTGTTTTTTATTTTAAGCTATT
TTGGAAAATATTTAATAGGA

Product: thiamine monphosphate kinase ThiL

Products: NA

Alternate protein names: Thiamine-phosphate kinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDEMKVIEIIKKTLKFSNENIVKGIDDDCAIIKIDENFYLVATTDMMVKKAHIPSILSPYEIGGRILTANVSDIASMGAK
PLAFLVSISLSKEEANEKFIKELYSGLDDFSKLYDCPVVGGDTNRGDELILSGTAFGITDNPIYRRGKVGDDICVTNDLG
RVYCALTLYYMLKENKISYKEFERLCQKYPKIIEKLRKPIARIKEGLLMNKLINGCCDISDGLGKEITYFKNFEIYSDRI
FKLIPEDVIEFCDAFNLNPIKVALNSGEEFELLFTTSKFNKVKDSLKGYSKIYKIGKIIEDGQFIDGEEFYGGGYIHKW

Sequences:

>Translated_319_residues
MDEMKVIEIIKKTLKFSNENIVKGIDDDCAIIKIDENFYLVATTDMMVKKAHIPSILSPYEIGGRILTANVSDIASMGAK
PLAFLVSISLSKEEANEKFIKELYSGLDDFSKLYDCPVVGGDTNRGDELILSGTAFGITDNPIYRRGKVGDDICVTNDLG
RVYCALTLYYMLKENKISYKEFERLCQKYPKIIEKLRKPIARIKEGLLMNKLINGCCDISDGLGKEITYFKNFEIYSDRI
FKLIPEDVIEFCDAFNLNPIKVALNSGEEFELLFTTSKFNKVKDSLKGYSKIYKIGKIIEDGQFIDGEEFYGGGYIHKW
>Mature_319_residues
MDEMKVIEIIKKTLKFSNENIVKGIDDDCAIIKIDENFYLVATTDMMVKKAHIPSILSPYEIGGRILTANVSDIASMGAK
PLAFLVSISLSKEEANEKFIKELYSGLDDFSKLYDCPVVGGDTNRGDELILSGTAFGITDNPIYRRGKVGDDICVTNDLG
RVYCALTLYYMLKENKISYKEFERLCQKYPKIIEKLRKPIARIKEGLLMNKLINGCCDISDGLGKEITYFKNFEIYSDRI
FKLIPEDVIEFCDAFNLNPIKVALNSGEEFELLFTTSKFNKVKDSLKGYSKIYKIGKIIEDGQFIDGEEFYGGGYIHKW

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family

Homologues:

Organism=Escherichia coli, GI1786619, Length=287, Percent_Identity=28.2229965156794, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_METJA (Q60337)

Other databases:

- EMBL:   L77117
- PIR:   D64303
- RefSeq:   NP_246991.1
- ProteinModelPortal:   Q60337
- SMR:   Q60337
- GeneID:   1450866
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0028
- NMPDR:   fig|243232.1.peg.28
- TIGR:   MJ0028
- HOGENOM:   HBG577902
- OMA:   ANVSDIA
- ProtClustDB:   CLSK876040
- BioCyc:   MJAN243232:MJ_0028-MONOMER
- BRENDA:   2.7.4.16
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283
- PIRSF:   PIRSF005303
- TIGRFAMs:   TIGR01379

Pfam domain/function: PF00586 AIRS; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =2.7.4.16

Molecular weight: Translated: 36160; Mature: 36160

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEMKVIEIIKKTLKFSNENIVKGIDDDCAIIKIDENFYLVATTDMMVKKAHIPSILSPY
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHCCCCCCCHH
EIGGRILTANVSDIASMGAKPLAFLVSISLSKEEANEKFIKELYSGLDDFSKLYDCPVVG
HHCCEEEEECHHHHHHCCCCCEEEHEEEECCHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
GDTNRGDELILSGTAFGITDNPIYRRGKVGDDICVTNDLGRVYCALTLYYMLKENKISYK
CCCCCCCEEEEECEEECCCCCCCEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHH
EFERLCQKYPKIIEKLRKPIARIKEGLLMNKLINGCCDISDGLGKEITYFKNFEIYSDRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHH
FKLIPEDVIEFCDAFNLNPIKVALNSGEEFELLFTTSKFNKVKDSLKGYSKIYKIGKIIE
HHHCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHC
DGQFIDGEEFYGGGYIHKW
CCCEECCCCCCCCCCEECC
>Mature Secondary Structure
MDEMKVIEIIKKTLKFSNENIVKGIDDDCAIIKIDENFYLVATTDMMVKKAHIPSILSPY
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHCCCCCCCHH
EIGGRILTANVSDIASMGAKPLAFLVSISLSKEEANEKFIKELYSGLDDFSKLYDCPVVG
HHCCEEEEECHHHHHHCCCCCEEEHEEEECCHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
GDTNRGDELILSGTAFGITDNPIYRRGKVGDDICVTNDLGRVYCALTLYYMLKENKISYK
CCCCCCCEEEEECEEECCCCCCCEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHH
EFERLCQKYPKIIEKLRKPIARIKEGLLMNKLINGCCDISDGLGKEITYFKNFEIYSDRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHH
FKLIPEDVIEFCDAFNLNPIKVALNSGEEFELLFTTSKFNKVKDSLKGYSKIYKIGKIIE
HHHCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHC
DGQFIDGEEFYGGGYIHKW
CCCEECCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087