Click here to switch to the map view.

The map label for this gene is fdhF [C]

Identifier: 15668178

GI number: 15668178

Start: 7338

End: 8474

Strand: Reverse

Name: fdhF [C]

Synonym: MJ0006

Alternate gene names: 15668178

Gene position: 8474-7338 (Counterclockwise)

Preceding gene: 15668184

Following gene: 15668177

Centisome position: 0.51

GC content: 32.63

Gene sequence:

>1137_bases
ATGAAAGTTGTTCATACTATCTGCCCAGGTTGTAGTGTTGGATGTGGAATTGATTTGATTGTTAAAGATGATAAAGTTGT
TGGCACTTATCCATACAAGAGACATCCAATAAATGAAGGTAAAAACTGTTCAAATGGAAAAAATAGCTATAAAATAATCT
ATCATGAAAAGAGATTAAAAAAGCCATTGATTAAGAAGAATGGAAAGCTTGTTGAAGCTACATGGGATGAGGCTTTAAGC
TTTATTGCAGAGAAATTAAAGAATTATAATGCTGATGATATAACCTTCATAGCCTCTGGAAAATGCACAAATGAAGATAA
CTACGCATTAAAAAAGTTGGTTGATAGCTTAAAAGCTAAAATTGGGCATTGTATCTGCAACTCTCCAAAGGTAAATTATG
CTGAGGTTTCTACAACAATTGATGATATTGAGAACGCAAAAAACATTATAATTATTGGTGATGTCTTCTCTGAACATGCG
TTAATTGGTAGAAAAGTTATTAAGGCAAAAGAAAAAGGATCTAAGGTAACAATTTTTAACACAGAGGAGAAGGAAATCCT
AAAGCTAAATGCCGATGAATTTGTGAAGGTTGATAGTTATTTAGGTGTTGATTTGAGTAATGTCGATAAAAATACCATTA
TCATAATTAATGCCCCAGTAAATGTTGATGAGATAATTAAAACTGCAAAGGAAAATAAAGCTAAGGTTTTGCCAGTTGCG
AAGCACTGCAATACAGTTGGAGCAACACTTATAGGCATCCCTGCTTTAAATAAGGATGAATATTTTGAATTATTAAAAAA
TTCAAAGTTCTTATACATAATGGGAGAGAATCCAGCTTTAGTTGATAAAGATGTCTTAAAAAATGTTGAATTTTTAGTTG
TCCAAGATATTATAATGACTGAGACAGCGGAGATGGCAGATGTTGTTTTGCCTTCAACATGCTGGGCTGAAAAGGATGGG
ACGTTTATAAACACTGATAAGAGAATTCAGAAAATAAATAAAGCTGTAAATCCTCCTGGAGATGCTATGGATGATTGGCT
TATAATAAAAAGCTTAGCTGAAAAGCTTGGTAGTGATTTGGGCTTTAACTCCTTAGAGGATATACAACAGGATATTCACA
GGAATAAACTTCTATAA

Upstream 100 bases:

>100_bases
AAGATTAGTAAGATAAAGGAAAGCTAGAAATCAAAATTAAAACTTAGACAAAAATAAACACAGAGAATTTTTATGACAAA
TAGAACATTTAGGTGATATT

Downstream 100 bases:

>100_bases
GGAAATTGACGCTTTTAGGCATCTAAATACCTTGAGGATATATAAACTGTGAAAGTCCTGTTTAAAAGAAATAACTAAGG
TGATATCATGAAATACGTTT

Product: formate dehydrogenase subunit alpha

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKVVHTICPGCSVGCGIDLIVKDDKVVGTYPYKRHPINEGKNCSNGKNSYKIIYHEKRLKKPLIKKNGKLVEATWDEALS
FIAEKLKNYNADDITFIASGKCTNEDNYALKKLVDSLKAKIGHCICNSPKVNYAEVSTTIDDIENAKNIIIIGDVFSEHA
LIGRKVIKAKEKGSKVTIFNTEEKEILKLNADEFVKVDSYLGVDLSNVDKNTIIIINAPVNVDEIIKTAKENKAKVLPVA
KHCNTVGATLIGIPALNKDEYFELLKNSKFLYIMGENPALVDKDVLKNVEFLVVQDIIMTETAEMADVVLPSTCWAEKDG
TFINTDKRIQKINKAVNPPGDAMDDWLIIKSLAEKLGSDLGFNSLEDIQQDIHRNKLL

Sequences:

>Translated_378_residues
MKVVHTICPGCSVGCGIDLIVKDDKVVGTYPYKRHPINEGKNCSNGKNSYKIIYHEKRLKKPLIKKNGKLVEATWDEALS
FIAEKLKNYNADDITFIASGKCTNEDNYALKKLVDSLKAKIGHCICNSPKVNYAEVSTTIDDIENAKNIIIIGDVFSEHA
LIGRKVIKAKEKGSKVTIFNTEEKEILKLNADEFVKVDSYLGVDLSNVDKNTIIIINAPVNVDEIIKTAKENKAKVLPVA
KHCNTVGATLIGIPALNKDEYFELLKNSKFLYIMGENPALVDKDVLKNVEFLVVQDIIMTETAEMADVVLPSTCWAEKDG
TFINTDKRIQKINKAVNPPGDAMDDWLIIKSLAEKLGSDLGFNSLEDIQQDIHRNKLL
>Mature_378_residues
MKVVHTICPGCSVGCGIDLIVKDDKVVGTYPYKRHPINEGKNCSNGKNSYKIIYHEKRLKKPLIKKNGKLVEATWDEALS
FIAEKLKNYNADDITFIASGKCTNEDNYALKKLVDSLKAKIGHCICNSPKVNYAEVSTTIDDIENAKNIIIIGDVFSEHA
LIGRKVIKAKEKGSKVTIFNTEEKEILKLNADEFVKVDSYLGVDLSNVDKNTIIIINAPVNVDEIIKTAKENKAKVLPVA
KHCNTVGATLIGIPALNKDEYFELLKNSKFLYIMGENPALVDKDVLKNVEFLVVQDIIMTETAEMADVVLPSTCWAEKDG
TFINTDKRIQKINKAVNPPGDAMDDWLIIKSLAEKLGSDLGFNSLEDIQQDIHRNKLL

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family

Homologues:

Organism=Homo sapiens, GI33519475, Length=109, Percent_Identity=33.9449541284404, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI3868721, Length=108, Percent_Identity=38.8888888888889, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788534, Length=194, Percent_Identity=26.2886597938144, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17565758, Length=105, Percent_Identity=42.8571428571429, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640559, Length=355, Percent_Identity=25.3521126760563, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24640557, Length=355, Percent_Identity=25.3521126760563, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y006_METJA (Q60314)

Other databases:

- EMBL:   L77117
- PIR:   F64300
- RefSeq:   NP_246969.1
- ProteinModelPortal:   Q60314
- GeneID:   1450845
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0006
- NMPDR:   fig|243232.1.peg.6
- TIGR:   MJ0006
- OMA:   FSEHALI
- ProtClustDB:   CLSK2517534
- BioCyc:   MJAN243232:MJ_0006-MONOMER
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- SMART:   SM00926

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin

EC number: 1.2.1.2

Molecular weight: Translated: 42051; Mature: 42051

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVHTICPGCSVGCGIDLIVKDDKVVGTYPYKRHPINEGKNCSNGKNSYKIIYHEKRLK
CCEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHH
KPLIKKNGKLVEATWDEALSFIAEKLKNYNADDITFIASGKCTNEDNYALKKLVDSLKAK
CHHHHCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
IGHCICNSPKVNYAEVSTTIDDIENAKNIIIIGDVFSEHALIGRKVIKAKEKGSKVTIFN
HHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEE
TEEKEILKLNADEFVKVDSYLGVDLSNVDKNTIIIINAPVNVDEIIKTAKENKAKVLPVA
CCCCEEEEECCHHHEEEHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEHH
KHCNTVGATLIGIPALNKDEYFELLKNSKFLYIMGENPALVDKDVLKNVEFLVVQDIIMT
HHCCHHCCCEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECHHHHCCCHHHHHHHHHHH
ETAEMADVVLPSTCWAEKDGTFINTDKRIQKINKAVNPPGDAMDDWLIIKSLAEKLGSDL
CHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
GFNSLEDIQQDIHRNKLL
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVHTICPGCSVGCGIDLIVKDDKVVGTYPYKRHPINEGKNCSNGKNSYKIIYHEKRLK
CCEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHH
KPLIKKNGKLVEATWDEALSFIAEKLKNYNADDITFIASGKCTNEDNYALKKLVDSLKAK
CHHHHCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
IGHCICNSPKVNYAEVSTTIDDIENAKNIIIIGDVFSEHALIGRKVIKAKEKGSKVTIFN
HHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEE
TEEKEILKLNADEFVKVDSYLGVDLSNVDKNTIIIINAPVNVDEIIKTAKENKAKVLPVA
CCCCEEEEECCHHHEEEHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEHH
KHCNTVGATLIGIPALNKDEYFELLKNSKFLYIMGENPALVDKDVLKNVEFLVVQDIIMT
HHCCHHCCCEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECHHHHCCCHHHHHHHHHHH
ETAEMADVVLPSTCWAEKDGTFINTDKRIQKINKAVNPPGDAMDDWLIIKSLAEKLGSDL
CHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
GFNSLEDIQQDIHRNKLL
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087