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The map label for this gene is radA

Identifier: 30995458

GI number: 30995458

Start: 1663821

End: 1665197

Strand: Reverse

Name: radA

Synonym: HI1597

Alternate gene names: 30995458

Gene position: 1665197-1663821 (Counterclockwise)

Preceding gene: 16273488

Following gene: 161350018

Centisome position: 90.99

GC content: 43.5

Gene sequence:

>1377_bases
ATGGCTAAAGCCCCAAAAACAGCTTATGTATGTAACGATTGTGGTGCGGAGTTTTCTCGTTGGCAAGGGCAATGTTCCGC
TTGTAAAGCGTGGAATACAATTACGGAAGTGCGGTTAATTTCCACGGCAAAATCAAAAAATGATCGTTTTAGTGGCTATG
CGGGTGAAACTCAGGCAAAAATTCAAACGCTCTCTGAAATTAGTTTGCAAGAAACACCACGTTTTTCCAGTGGTTTTAGT
GAATTAGATAGGGTACTGGGCGGCGGGATCGTACCGGGTAGTGCGATTTTAATTGGTGGGCATCCTGGAGCAGGGAAAAG
TACATTGTTACTACAGGTTATGTGTGGTTTGGCAAAAAATATGACCGCACTTTATGTAACGGGAGAGGAATCATTACAAC
AGGTTGCGATGCGCGCAAGCCGATTGGGATTGCCAAGTGATCAATTAAAAATGTTGTCAGAAACCTCTGTGGAACAGATT
TGTAATTTAGCGGATCAATTAAAACCGCAAATTATCGTGGTGGATTCTATCCAAGTAATGCATTTGGCAGATATTCAATC
ATCGCCAGGCAGTGTAGCTCAAGTGCGTGAATGCGCTTCTTTTCTCACTCGTTATGCAAAAACTCGCCAAGTGGCGATTA
TTATGGTTGGACACGTTACGAAAGACGGAACCCTTGCAGGGCCTAAAGTGTTAGAGCATGCCATTGACTGTTCATTGTTA
TTAGAAGGCGAAGCCGATTCTCGTTATCGTACTTTGCGTAGTCATAAAAACCGTTTTGGTGCAGTAAATGAATTGGGCGT
GTTTGGAATGACAGAGCAGGGTTTGCGCGAAGTGAAAAATCCATCGGCGATTTTTTTAAGTCGCGGAGATGAAATTACGT
CAGGCAGTTCTGTTATGGTACTTTGGGAGGGTACTCGTCCTCTTTTAGTTGAAATTCAAGCTTTAGTAGATCATTCAATG
CTGGCAAATCCTCGCCGTGTTGCGGTGGGATTAGAGCAAAATCGCTTAGCGCTATTACTGGCGGTGTTACACCGACATGG
TGGCTTACAAATGGCGGATCAAGATGTTTTTGTAAATGTGGTTGGTGGTGTAAAAGTCAGTGAAACCAGTGCGGATTTAG
CTTTGTTGCTTGCGTTAATTTCAAGTTTCCGTAATCGCCCTTTGCCGCAAGATTTAGTGATTTTTGGCGAAGTTGGATTA
GCGGGAGAAATTCGTCCTGTGCCAAGTGGACAGGAACGTATTAGCGAGGCTGCAAAACACGGTTTTAAACGTGCTATTGT
TCCTTTTGGTAACAAGCCGAAAAGTGCGGTCGAAAATATGCAGGTTTTTACTGTGAAAAAACTCACGGATGCGCTCGCTG
TGTTAGATAATTTGTAA

Upstream 100 bases:

>100_bases
GGGTAGTTTGATGTTCCCCAAAGGGGAACCACTATAACTATAATTAACCGTGGGTATACTTACCCACGGGAAGTTATCCA
CGGCAAATACGGAGAATAAA

Downstream 100 bases:

>100_bases
AAAAATCATCGCCTACTGAAAATGAAAGGAAAATTCTGTAGAATAGTTGTTCCTTTTTATGAGCAAAGAAATAAAGAAAA
TGGAAAAAAAACGAAATAAA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAKAPKTAYVCNDCGAEFSRWQGQCSACKAWNTITEVRLISTAKSKNDRFSGYAGETQAKIQTLSEISLQETPRFSSGFS
ELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLAKNMTALYVTGEESLQQVAMRASRLGLPSDQLKMLSETSVEQI
CNLADQLKPQIIVVDSIQVMHLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDCSLL
LEGEADSRYRTLRSHKNRFGAVNELGVFGMTEQGLREVKNPSAIFLSRGDEITSGSSVMVLWEGTRPLLVEIQALVDHSM
LANPRRVAVGLEQNRLALLLAVLHRHGGLQMADQDVFVNVVGGVKVSETSADLALLLALISSFRNRPLPQDLVIFGEVGL
AGEIRPVPSGQERISEAAKHGFKRAIVPFGNKPKSAVENMQVFTVKKLTDALAVLDNL

Sequences:

>Translated_458_residues
MAKAPKTAYVCNDCGAEFSRWQGQCSACKAWNTITEVRLISTAKSKNDRFSGYAGETQAKIQTLSEISLQETPRFSSGFS
ELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLAKNMTALYVTGEESLQQVAMRASRLGLPSDQLKMLSETSVEQI
CNLADQLKPQIIVVDSIQVMHLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDCSLL
LEGEADSRYRTLRSHKNRFGAVNELGVFGMTEQGLREVKNPSAIFLSRGDEITSGSSVMVLWEGTRPLLVEIQALVDHSM
LANPRRVAVGLEQNRLALLLAVLHRHGGLQMADQDVFVNVVGGVKVSETSADLALLLALISSFRNRPLPQDLVIFGEVGL
AGEIRPVPSGQERISEAAKHGFKRAIVPFGNKPKSAVENMQVFTVKKLTDALAVLDNL
>Mature_457_residues
AKAPKTAYVCNDCGAEFSRWQGQCSACKAWNTITEVRLISTAKSKNDRFSGYAGETQAKIQTLSEISLQETPRFSSGFSE
LDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLAKNMTALYVTGEESLQQVAMRASRLGLPSDQLKMLSETSVEQIC
NLADQLKPQIIVVDSIQVMHLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDCSLLL
EGEADSRYRTLRSHKNRFGAVNELGVFGMTEQGLREVKNPSAIFLSRGDEITSGSSVMVLWEGTRPLLVEIQALVDHSML
ANPRRVAVGLEQNRLALLLAVLHRHGGLQMADQDVFVNVVGGVKVSETSADLALLLALISSFRNRPLPQDLVIFGEVGLA
GEIRPVPSGQERISEAAKHGFKRAIVPFGNKPKSAVENMQVFTVKKLTDALAVLDNL

Specific function: May play a role in the repair of endogenous alkylation damage

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=80, Blast_Score=776, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RADA_HAEIN (P45266)

Other databases:

- EMBL:   L42023
- PIR:   I64131
- RefSeq:   NP_439739.2
- ProteinModelPortal:   P45266
- SMR:   P45266
- GeneID:   950453
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1597
- NMPDR:   fig|71421.1.peg.1513
- TIGR:   HI_1597
- HOGENOM:   HBG286573
- OMA:   IGVFEMT
- ProtClustDB:   PRK11823
- BioCyc:   HINF71421:HI_1597-MONOMER
- GO:   GO:0006508
- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568
- PRINTS:   PR01874
- SMART:   SM00382
- TIGRFAMs:   TIGR00416

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: NA

Molecular weight: Translated: 49372; Mature: 49240

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPKTAYVCNDCGAEFSRWQGQCSACKAWNTITEVRLISTAKSKNDRFSGYAGETQAK
CCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHEEHHHCCCCCCCCCCCCCHHHH
IQTLSEISLQETPRFSSGFSELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLAKN
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCC
MTALYVTGEESLQQVAMRASRLGLPSDQLKMLSETSVEQICNLADQLKPQIIVVDSIQVM
CEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
HLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDCSLL
EEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHHHCCCEEE
LEGEADSRYRTLRSHKNRFGAVNELGVFGMTEQGLREVKNPSAIFLSRGDEITSGSSVMV
EECCCCHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEEE
LWEGTRPLLVEIQALVDHSMLANPRRVAVGLEQNRLALLLAVLHRHGGLQMADQDVFVNV
EECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCEEEEE
VGGVKVSETSADLALLLALISSFRNRPLPQDLVIFGEVGLAGEIRPVPSGQERISEAAKH
ECCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHC
GFKRAIVPFGNKPKSAVENMQVFTVKKLTDALAVLDNL
CCCEEECCCCCCCHHHHCCCCEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAPKTAYVCNDCGAEFSRWQGQCSACKAWNTITEVRLISTAKSKNDRFSGYAGETQAK
CCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHEEHHHCCCCCCCCCCCCCHHHH
IQTLSEISLQETPRFSSGFSELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLAKN
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCC
MTALYVTGEESLQQVAMRASRLGLPSDQLKMLSETSVEQICNLADQLKPQIIVVDSIQVM
CEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
HLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDCSLL
EEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHHHCCCEEE
LEGEADSRYRTLRSHKNRFGAVNELGVFGMTEQGLREVKNPSAIFLSRGDEITSGSSVMV
EECCCCHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEEE
LWEGTRPLLVEIQALVDHSMLANPRRVAVGLEQNRLALLLAVLHRHGGLQMADQDVFVNV
EECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCEEEEE
VGGVKVSETSADLALLLALISSFRNRPLPQDLVIFGEVGLAGEIRPVPSGQERISEAAKH
ECCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHC
GFKRAIVPFGNKPKSAVENMQVFTVKKLTDALAVLDNL
CCCEEECCCCCCCHHHHCCCCEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800